使用膜进样质谱仪评估 O2:Ar、N2:Ar 和 29,30N2 的情况,该质谱仪的配置可最大限度地减少氧气干扰

IF 2.1 3区 地球科学 Q2 LIMNOLOGY
Ashlynn R. Boedecker, Jason M. Taylor, Tyler H. Tappenbeck, Robert O. Hall Jr., Caleb J. Robbins, J. Thad Scott
{"title":"使用膜进样质谱仪评估 O2:Ar、N2:Ar 和 29,30N2 的情况,该质谱仪的配置可最大限度地减少氧气干扰","authors":"Ashlynn R. Boedecker,&nbsp;Jason M. Taylor,&nbsp;Tyler H. Tappenbeck,&nbsp;Robert O. Hall Jr.,&nbsp;Caleb J. Robbins,&nbsp;J. Thad Scott","doi":"10.1002/lom3.10644","DOIUrl":null,"url":null,"abstract":"<p>Membrane inlet mass spectrometry (MIMS) provides detailed measures of dissolved <sup>28,29,30</sup>N<sub>2</sub>, O<sub>2</sub>, and argon (Ar) for estimating important gas fluxes and concentrations in aquatic ecosystems. Previous studies demonstrated a large O<sub>2</sub> scavenging effect while using a MIMS, where varying concentrations of O<sub>2</sub> can affect measured N<sub>2</sub> : Ar because O<sub>2</sub> interacts with N<sub>2</sub> in the ion source to produce NO<sup>+</sup> (<i>m</i>/<i>z</i> = 30), potentially decreasing the detected current for <sup>28,29</sup>N<sub>2</sub> and increasing the detected current for <sup>30</sup>N<sub>2</sub>. A common solution is to use a muffle furnace heated to 600°C with a copper reduction column to reduce the concentration of O<sub>2</sub> to minimal levels and accurately measure <sup>28,29,30</sup>N<sub>2</sub>. However, this solution eliminates the detection of O<sub>2</sub> in environmental samples, which is a major benefit of using a MIMS. We questioned whether the MIMS was sensitive enough to provide accurate O<sub>2</sub> estimates when using the furnace and whether the O<sub>2</sub> scavenging effect was real and consistent among MIMS. We conducted four separate experiments on three different MIMS to test the O<sub>2</sub> scavenging effect and the potential detection of O<sub>2</sub> when using a MIMS with furnace. The furnace removed ~ 99% of O<sub>2</sub>, and O<sub>2</sub> scavenging had little to no detectable effect on N<sub>2</sub> : Ar and an unclear effect on <sup>29</sup>N<sub>2</sub> : <sup>28</sup>N<sub>2</sub>, but increased <sup>30</sup>N<sub>2</sub> : <sup>28</sup>N<sub>2</sub>. In most cases, accurate O<sub>2</sub> data could be retrieved despite using the furnace. The need for O<sub>2</sub> reduction may be limited to measuring accurate <sup>30</sup>N<sub>2</sub> : <sup>28</sup>N<sub>2</sub> in isotope pairing studies, but without substantial loss of MIMS measurements used to describe O<sub>2</sub> dynamics.</p>","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"22 11","pages":"791-804"},"PeriodicalIF":2.1000,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lom3.10644","citationCount":"0","resultStr":"{\"title\":\"Evaluating O2 : Ar, N2 : Ar, and 29,30N2 using membrane inlet mass spectrometry configured to minimize oxygen interference\",\"authors\":\"Ashlynn R. Boedecker,&nbsp;Jason M. Taylor,&nbsp;Tyler H. Tappenbeck,&nbsp;Robert O. Hall Jr.,&nbsp;Caleb J. Robbins,&nbsp;J. Thad Scott\",\"doi\":\"10.1002/lom3.10644\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Membrane inlet mass spectrometry (MIMS) provides detailed measures of dissolved <sup>28,29,30</sup>N<sub>2</sub>, O<sub>2</sub>, and argon (Ar) for estimating important gas fluxes and concentrations in aquatic ecosystems. Previous studies demonstrated a large O<sub>2</sub> scavenging effect while using a MIMS, where varying concentrations of O<sub>2</sub> can affect measured N<sub>2</sub> : Ar because O<sub>2</sub> interacts with N<sub>2</sub> in the ion source to produce NO<sup>+</sup> (<i>m</i>/<i>z</i> = 30), potentially decreasing the detected current for <sup>28,29</sup>N<sub>2</sub> and increasing the detected current for <sup>30</sup>N<sub>2</sub>. A common solution is to use a muffle furnace heated to 600°C with a copper reduction column to reduce the concentration of O<sub>2</sub> to minimal levels and accurately measure <sup>28,29,30</sup>N<sub>2</sub>. However, this solution eliminates the detection of O<sub>2</sub> in environmental samples, which is a major benefit of using a MIMS. We questioned whether the MIMS was sensitive enough to provide accurate O<sub>2</sub> estimates when using the furnace and whether the O<sub>2</sub> scavenging effect was real and consistent among MIMS. We conducted four separate experiments on three different MIMS to test the O<sub>2</sub> scavenging effect and the potential detection of O<sub>2</sub> when using a MIMS with furnace. The furnace removed ~ 99% of O<sub>2</sub>, and O<sub>2</sub> scavenging had little to no detectable effect on N<sub>2</sub> : Ar and an unclear effect on <sup>29</sup>N<sub>2</sub> : <sup>28</sup>N<sub>2</sub>, but increased <sup>30</sup>N<sub>2</sub> : <sup>28</sup>N<sub>2</sub>. In most cases, accurate O<sub>2</sub> data could be retrieved despite using the furnace. The need for O<sub>2</sub> reduction may be limited to measuring accurate <sup>30</sup>N<sub>2</sub> : <sup>28</sup>N<sub>2</sub> in isotope pairing studies, but without substantial loss of MIMS measurements used to describe O<sub>2</sub> dynamics.</p>\",\"PeriodicalId\":18145,\"journal\":{\"name\":\"Limnology and Oceanography: Methods\",\"volume\":\"22 11\",\"pages\":\"791-804\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-08-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lom3.10644\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Limnology and Oceanography: Methods\",\"FirstCategoryId\":\"89\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/lom3.10644\",\"RegionNum\":3,\"RegionCategory\":\"地球科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"LIMNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Limnology and Oceanography: Methods","FirstCategoryId":"89","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/lom3.10644","RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"LIMNOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

膜进样质谱法(MIMS)可详细测量溶解的 28、29、30N2、O2 和氩气(Ar),用于估算水生生态系统中的重要气体通量和浓度。以往的研究表明,使用 MIMS 时会产生很大的 O2 清除效应,不同浓度的 O2 会影响 N2 : Ar 的测量值,因为 O2 会与离子源中的 N2 相互作用,产生 NO+(m/z = 30),从而可能降低 28、29N2 的检测电流,增加 30N2 的检测电流。常见的解决方案是使用加热至 600°C 的马弗炉和铜还原柱,将 O2 浓度降至最低水平,从而准确测量 28、29、30N2。然而,这种解决方案无法检测环境样本中的 O2,而这正是使用多级多光谱仪的一大好处。我们质疑 MIMS 的灵敏度是否足以在使用熔炉时提供准确的 O2 估计值,以及 O2 清除效果是否真实,并且在 MIMS 之间是否一致。我们在三个不同的 MIMS 上分别进行了四次实验,以测试使用带熔炉的 MIMS 时的 O2 清除效果和潜在的 O2 检测能力。熔炉清除了约 99% 的 O2,O2 清除对 N2 : Ar 几乎没有检测到影响,对 29N2 : 28N2 的影响不明显,但增加了 30N2 : 28N2。在大多数情况下,尽管使用了熔炉,但仍能获得准确的氧气数据。减少 O2 的需要可能仅限于在同位素配对研究中测量准确的 30N2 : 28N2,但不会大量损失用于描述 O2 动态的 MIMS 测量值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Evaluating O2 : Ar, N2 : Ar, and 29,30N2 using membrane inlet mass spectrometry configured to minimize oxygen interference

Evaluating O2 : Ar, N2 : Ar, and 29,30N2 using membrane inlet mass spectrometry configured to minimize oxygen interference

Membrane inlet mass spectrometry (MIMS) provides detailed measures of dissolved 28,29,30N2, O2, and argon (Ar) for estimating important gas fluxes and concentrations in aquatic ecosystems. Previous studies demonstrated a large O2 scavenging effect while using a MIMS, where varying concentrations of O2 can affect measured N2 : Ar because O2 interacts with N2 in the ion source to produce NO+ (m/z = 30), potentially decreasing the detected current for 28,29N2 and increasing the detected current for 30N2. A common solution is to use a muffle furnace heated to 600°C with a copper reduction column to reduce the concentration of O2 to minimal levels and accurately measure 28,29,30N2. However, this solution eliminates the detection of O2 in environmental samples, which is a major benefit of using a MIMS. We questioned whether the MIMS was sensitive enough to provide accurate O2 estimates when using the furnace and whether the O2 scavenging effect was real and consistent among MIMS. We conducted four separate experiments on three different MIMS to test the O2 scavenging effect and the potential detection of O2 when using a MIMS with furnace. The furnace removed ~ 99% of O2, and O2 scavenging had little to no detectable effect on N2 : Ar and an unclear effect on 29N2 : 28N2, but increased 30N2 : 28N2. In most cases, accurate O2 data could be retrieved despite using the furnace. The need for O2 reduction may be limited to measuring accurate 30N2 : 28N2 in isotope pairing studies, but without substantial loss of MIMS measurements used to describe O2 dynamics.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
4.80
自引率
3.70%
发文量
56
审稿时长
3 months
期刊介绍: Limnology and Oceanography: Methods (ISSN 1541-5856) is a companion to ASLO''s top-rated journal Limnology and Oceanography, and articles are held to the same high standards. In order to provide the most rapid publication consistent with high standards, Limnology and Oceanography: Methods appears in electronic format only, and the entire submission and review system is online. Articles are posted as soon as they are accepted and formatted for publication. Limnology and Oceanography: Methods will consider manuscripts whose primary focus is methodological, and that deal with problems in the aquatic sciences. Manuscripts may present new measurement equipment, techniques for analyzing observations or samples, methods for understanding and interpreting information, analyses of metadata to examine the effectiveness of approaches, invited and contributed reviews and syntheses, and techniques for communicating and teaching in the aquatic sciences.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信