Xinyuan Yuan, Tingting Wu, Teliang Lu, Jiandong Ye
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引用次数: 0
摘要
硅（Si）和锌（Zn）离子都是骨骼健康的重要元素，它们促进成骨的机制引起了研究人员的广泛关注。然而，双离子促进成骨分化的机制仍有待阐明。本文首先研究了 Si 和 Zn 离子对 mBMSCs 细胞学行为的影响。然后，通过转录组测序技术研究了Si-Zn双离子调控mBMSCs成骨分化的分子机制。在单离子体系中，浓度为1.5 mM（Si-1.5）的Si离子对细胞增殖、ALP活性和成骨相关基因（ALP、Runx2、OCN、Col-I和BSP）表达水平有较好的综合效应；浓度为50 μM（Zn-50）的Zn离子对细胞增殖、ALP活性和相同的成骨基因表达水平有较好的综合效应。在双离子系统中，与单离子组相比，Si（1.5 mM）-Zn（50 μM）组（Si1.5-Zn50）能合成性地提高 ALP 活性和成骨基因。转录组测序分析结果表明，硅离子对促进 mBMSCs 成骨分化有一定作用；与硅离子相比，锌离子对促进 mBMSCs 成骨分化的作用更强；与单离子（硅或锌）相比，硅锌双离子对促进 mBMSCs 成骨分化有协同增强作用。该研究为探索双离子对成骨分化的调控机制提供了蓝图。 图文摘要
Si and Zn dual ions upregulate the osteogenic differentiation of mBMSCs: mRNA transcriptomic sequencing analysis
Both silicon (Si) and zinc (Zn) ions are essential elements to bone health and their mechanisms for promoting osteogenesis have aroused the extensive attention of researchers. Thereinto, the mechanism by which dual ions promote osteogenic differentiation remains to be elucidated. Herein, the effects of Si and Zn ions on the cytological behaviors of mBMSCs were firstly studied. Then, the molecular mechanism of Si-Zn dual ions regulating the osteogenic differentiation of mBMSCs was investigated via transcriptome sequencing technology. In the single-ion system, Si ion at the concentration of 1.5 mM (Si-1.5) had better comprehensive effects of cell proliferation, ALP activity and osteogenesis-related gene expression levels (ALP, Runx2, OCN, Col-I and BSP); Zn ion at the concentration of 50 μM (Zn-50) demonstrated better combining effects of cell proliferation, ALP activity and same osteogenic genes expression levels. In the dual-ion system, the Si (1.5 mM)-Zn (50 μM) group (Si1.5-Zn50) synthetically enhanced ALP activity and osteogenesis genes compared with single-ion groups. Analysis of the transcriptome sequencing results showed that Si ion had a certain effect on promoting the osteogenic differentiation of mBMSCs; Zn ion had a stronger effect of contributing to a better osteogenic differentiation of mBMSCs than that of Si ion; the Si-Zn dual ions had a synergistic enhancement on conducting to the osteogenic differentiation of mBMSCs compared to single ion (Si or Zn). This study offers a blueprint for exploring the regulation mechanism of osteogenic differentiation by dual ions.
期刊介绍：
The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.
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