评估作为生物材料的生物陶瓷磷酸盐的抗氧化活性、血栓形成性和 MTT 试验

IF 1.8 4区 材料科学 Q2 MATERIALS SCIENCE, CERAMICS
Ranbir Kumar, Deep Shikha, Sanjay Kumar Sinha, José R. Guerra-López, Neda Aboudzadeh
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引用次数: 0

摘要

羟基磷灰石(HAP)是骨骼和牙齿的基石,因其潜在的生物活性和治疗意义而备受关注。本研究全面评估了羟基磷灰石的多面性,深入研究了它的抗氧化性、血栓形成性和细胞相容性。HAP 通过溶胶-凝胶法合成,经能量色散 X 射线光谱(EDS)确认,其 Ca/P 比值为 1.66。X 射线衍射 (XRD) 验证了化合物的形成,而傅立叶变换红外光谱 (FTIR) 则确定了官能团。场发射电子显微镜揭示了 HAP 的表面形态。其结构特征揭示了 HAP 的功能潜力。利用 DPPH(2,2-二苯基-1-苦基肼)测定法,评估了纯 HAP 中和自由基(抗氧化活性的象征)的能力。此外,利用全血凝固试验进行的血栓形成研究探讨了 HAP 对凝血的影响,凝血是生物相容性的一个关键因素。在 L929 细胞系中采用 MTT(3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑)检测法进一步评估了 HAP 与活细胞的相互作用,从而揭示了 HAP 的细胞毒性或潜在的细胞活力增强作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Assessment of antioxidant activity, thrombogenicity and MTT assay of bioceramic phosphate as a biomaterial

Assessment of antioxidant activity, thrombogenicity and MTT assay of bioceramic phosphate as a biomaterial

Hydroxyapatite (HAP), a cornerstone of bone and teeth, has ignited interest due to its potential bioactivity and therapeutic implications. This study comprehensively assesses the multifaceted nature of HAP, delving into its antioxidant, thrombogenic, and cytocompatibility properties. HAP was synthesized via the sol-gel method, achieving a Ca/P ratio 1.66, confirmed by energy-dispersive X-ray spectroscopy (EDS). X-ray diffraction (XRD) verified compound formation, while Fourier transform infrared spectroscopy (FTIR) identified functional groups. Field emission electron microscopy revealed the surface morphology of HAP. Its structural characterization delves into HAP’s functional potential. Utilizing the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay, pure HAP’s ability to neutralize free radicals, a symbol of antioxidant activity, was evaluated. Additionally, thrombogenic studies using the whole blood clotting assay explored HAP’s influence on blood clotting, a crucial factor for biocompatibility. The MTT (3-(4, 5-dimethythiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) assay was employed in the L929 cell line to assess its interaction with living cells further, shedding light on HAP’s cytotoxicity or potential cell viability enhancement.

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来源期刊
Journal of the Australian Ceramic Society
Journal of the Australian Ceramic Society Materials Science-Materials Chemistry
CiteScore
3.70
自引率
5.30%
发文量
123
期刊介绍: Publishes high quality research and technical papers in all areas of ceramic and related materials Spans the broad and growing fields of ceramic technology, material science and bioceramics Chronicles new advances in ceramic materials, manufacturing processes and applications Journal of the Australian Ceramic Society since 1965 Professional language editing service is available through our affiliates Nature Research Editing Service and American Journal Experts at the author''s cost and does not guarantee that the manuscript will be reviewed or accepted
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