Y. M. Hamdan, D. A. Makarova, N. I. Shamsutdinov, P. V. Zelenikhin, A. S. Nizamutdinov, A. A. Buglak, T. A. Telegina
{"title":"紫外线激光脉冲持续时间和波长对成纤维细胞影响的研究","authors":"Y. M. Hamdan, D. A. Makarova, N. I. Shamsutdinov, P. V. Zelenikhin, A. S. Nizamutdinov, A. A. Buglak, T. A. Telegina","doi":"10.1134/s1547477124700717","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>We report the cytotoxic effect of pulse duration and wavelength of UVB laser radiation from Li(Lu,Y)F<sub>4</sub>:Ce laser on human skin fibroblasts. When performing the MTT test 24 h after irradiation, an increase in the viability parameter was observed. Flow cytometry showed that 80.8 ± 6.9% of cells have a damaged membrane and only 6.2 ± 2.2% reach the stage of late apoptosis, due to which they have the potential to restore the cytoplasmic membrane and subsequently divide.</p>","PeriodicalId":730,"journal":{"name":"Physics of Particles and Nuclei Letters","volume":"30 1","pages":""},"PeriodicalIF":0.4000,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Study of the Effect of UV Laser Pulse Duration and Wavelength on Fibroblasts\",\"authors\":\"Y. M. Hamdan, D. A. Makarova, N. I. Shamsutdinov, P. V. Zelenikhin, A. S. Nizamutdinov, A. A. Buglak, T. A. Telegina\",\"doi\":\"10.1134/s1547477124700717\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Abstract</h3><p>We report the cytotoxic effect of pulse duration and wavelength of UVB laser radiation from Li(Lu,Y)F<sub>4</sub>:Ce laser on human skin fibroblasts. When performing the MTT test 24 h after irradiation, an increase in the viability parameter was observed. Flow cytometry showed that 80.8 ± 6.9% of cells have a damaged membrane and only 6.2 ± 2.2% reach the stage of late apoptosis, due to which they have the potential to restore the cytoplasmic membrane and subsequently divide.</p>\",\"PeriodicalId\":730,\"journal\":{\"name\":\"Physics of Particles and Nuclei Letters\",\"volume\":\"30 1\",\"pages\":\"\"},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2024-08-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Physics of Particles and Nuclei Letters\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1134/s1547477124700717\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PHYSICS, PARTICLES & FIELDS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physics of Particles and Nuclei Letters","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1134/s1547477124700717","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHYSICS, PARTICLES & FIELDS","Score":null,"Total":0}
Study of the Effect of UV Laser Pulse Duration and Wavelength on Fibroblasts
Abstract
We report the cytotoxic effect of pulse duration and wavelength of UVB laser radiation from Li(Lu,Y)F4:Ce laser on human skin fibroblasts. When performing the MTT test 24 h after irradiation, an increase in the viability parameter was observed. Flow cytometry showed that 80.8 ± 6.9% of cells have a damaged membrane and only 6.2 ± 2.2% reach the stage of late apoptosis, due to which they have the potential to restore the cytoplasmic membrane and subsequently divide.
期刊介绍:
The journal Physics of Particles and Nuclei Letters, brief name Particles and Nuclei Letters, publishes the articles with results of the original theoretical, experimental, scientific-technical, methodological and applied research. Subject matter of articles covers: theoretical physics, elementary particle physics, relativistic nuclear physics, nuclear physics and related problems in other branches of physics, neutron physics, condensed matter physics, physics and engineering at low temperatures, physics and engineering of accelerators, physical experimental instruments and methods, physical computation experiments, applied research in these branches of physics and radiology, ecology and nuclear medicine.