用于治疗复发性流产的荧光、pH 值和 UCST 响应聚合物纳米材料

IF 2.6 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS
Xuanping Lu, Weiqin Zhou, Caiping Mao
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引用次数: 0

摘要

复发性流产(RM)是指连续三次或三次以上自然流产,影响着许多育龄妇女。RM的病理基础是细胞凋亡失衡,其中MDM2-p53通路起着至关重要的作用。在这项研究中,我们通过用 4-氨基苯并咪唑(4-ABI)修饰聚(6-乙酰氧基-ε-己内酰胺)(PCCL),合成了聚(6-乙酰氧基-ε-己内酰胺)-接枝-(4-氨基苯并咪唑)(PCCL-4-ABI)。羧基和 4-ABI 基团的引入赋予了 PCL 骨架荧光、pH 值响应性和 UCST 响应性。负载化合物 1 的 PCCL-4-ABI 纳米荧光材料随温度变化的释放行为归因于 UCST 过渡。我们成功开发了一种新型纳米荧光聚合物药物递送平台 PCCL-4-ABI@1,并评估了其对滋养层细胞(HTR-8/SVneo)中 p53 和 MDM2 的调控作用。结果表明,负载低分子量肝素的系统以剂量依赖的方式增加了 MDM2 的表达,降低了 p53 的表达,从而抑制了滋养层细胞的凋亡。该研究开发了一种具有 UCST 行为的可生物降解聚(ε-己内酯),对热致伸缩性荧光纳米粒子系统的发展具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Fluorescent, pH, and UCST Responsive Polymer Nanomaterials for Treatment of Recurrent Miscarriage

Fluorescent, pH, and UCST Responsive Polymer Nanomaterials for Treatment of Recurrent Miscarriage

Recurrent miscarriage (RM), defined as three or more consecutive spontaneous miscarriages, affects many women of childbearing age. The pathological basis of RM is an imbalance in apoptosis, with the MDM2-p53 pathway playing a crucial role. In this study, we synthesized poly(6-acetoxyl-ε-caprolactone)-graft-(4-amino-benzimidazole) (PCCL-4-ABI) by modifying poly(6-acetoxyl-ε-caprolactone) (PCCL) with 4-amino-benzimidazole (4-ABI). The introduction of carboxyl and 4-ABI groups endowed the PCL backbone with fluorescence, pH responsiveness, and UCST responsiveness. The temperature-dependent release behavior of compound 1-loaded PCCL-4-ABI nanofluorescent materials was attributed to UCST transition. We successfully developed a novel nanofluorescent polymer drug delivery platform, PCCL-4-ABI@1, and evaluated its regulatory effects on p53 and MDM2 in trophoblast cells (HTR-8/SVneo). The results showed that the system loaded with low molecular weight heparin increased MDM2 and decreased p53 expression in a dose-dependent manner, thereby inhibiting trophoblast cell apoptosis. This study developed a biodegradable poly(ε-caprolactone) with UCST behavior, significant for the advancement of thermoresponsive fluorescent nanoparticle systems.

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来源期刊
Journal of Fluorescence
Journal of Fluorescence 化学-分析化学
CiteScore
4.60
自引率
7.40%
发文量
203
审稿时长
5.4 months
期刊介绍: Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
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