开发用于慢性肾脏病血管和形态学评估的宽视场高分辨率双光学成像平台:可行性研究

IF 5.3 2区 医学 Q1 ENGINEERING, BIOMEDICAL
Sm Abu Saleah , Jaeyul Lee , Daewoon Seong , Sangyeob Han , Kibeom Park , Juyeon Hong , Sooah Park , Yoon-Hee Kwon , Woonggyu Jung , Mansik Jeon , Jeehyun Kim
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引用次数: 0

摘要

慢性肾脏病(CKD)会影响肾脏的形态结构并导致肾功能显著下降,从而导致患者接受肾脏替代治疗。血管稀疏被认为是加速 CKD 患者肾脏损伤的重要因素,因此,肾脏形态和血管的评估在肾脏病学中至关重要。本研究旨在评估 CKD 引起的小鼠肾脏形态和血管变化。本研究采用双光声学显微镜(PAM)和光学相干显微镜(OCM)为导向的宽视场高分辨率成像模式进行病变肾脏成像。利用单侧输尿管梗阻(UUO)模型制备 CKD 肾脏样本,并利用所开发的宽场双成像系统对对照肾脏和受 CKD 影响的肾脏进行成像,以评估 CKD 进展过程中的血管和形态变化。结果显示,随着 UUO 病的进展,血管强度和肾盂空间逐渐发生变化。此外,还根据血管的节点、交界处和网状结构对微血管进行了定量分析,从而详细了解了随着病情的发展,外周区域微血管相关特征的增加。因此,通过同时利用每种光学成像技术的优势,利用宽视场多模态成像系统评估肾脏样本的基于 OCM 的形态学和基于 PAM 的血管特性的拟议方法可以成为肾脏组织病理学中无需外源性造影剂的全容积分析的有效技术。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development of wide-field high-resolution dual optical imaging platform for vasculature and morphological assessment of chronic kidney disease: A feasibility study

Chronic kidney disease (CKD) affects the morphological structure and causes significant degradation in kidney function, leading to renal replacement treatment in affected individuals. Vascular rarefaction is thought to be an important factor in accelerating kidney damage in CKD patients, therefore, the assessment of renal morphology and vasculature is crucial in nephrology. The objective of this study was to evaluate the morphological and vascular changes caused by CKD in mice kidneys. In this study, dual photoacoustic microscopy (PAM) and optical coherence microscopy (OCM) oriented wide-field high-resolution imaging modalities were employed for diseased renal imaging. The unilateral ureteral obstruction (UUO) model was used to prepare renal samples with CKD, and the developed wide-field dual imaging system was used to image both control and CKD-affected kidneys for assessing vascular and morphological changes during CKD progression. The obtained results reveal a gradual alteration in vascular intensity and pelvis space with the progress of UUO disease. Furthermore, a quantitative micro-vessel analysis was performed based on the node, junction, and mesh of the vessel, which provides details on the increasing microvascular-related characteristics in the peripheral area as the disease progresses. Thus, by concurrently employing the advantages of each optical imaging technique, the proposed method of assessing the OCM-based morphological and PAM-based vascular properties of the renal sample using a wide-field multimodal imaging system can be an efficient technique for whole volume analysis without any exogenous contrast agents in kidney histopathology.

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来源期刊
CiteScore
16.50
自引率
6.20%
发文量
77
审稿时长
38 days
期刊介绍: Biocybernetics and Biomedical Engineering is a quarterly journal, founded in 1981, devoted to publishing the results of original, innovative and creative research investigations in the field of Biocybernetics and biomedical engineering, which bridges mathematical, physical, chemical and engineering methods and technology to analyse physiological processes in living organisms as well as to develop methods, devices and systems used in biology and medicine, mainly in medical diagnosis, monitoring systems and therapy. The Journal''s mission is to advance scientific discovery into new or improved standards of care, and promotion a wide-ranging exchange between science and its application to humans.
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