Di Wu , Miao-Miao Zhang , Hong-Ping Long , Xiao-Yuan Lin , Kai Liu
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引用次数: 0
摘要
利用高效液相色谱（HPLC）指纹图谱和多元数据分析，包括层次聚类分析（HCA）、主成分分析（PCA）和正交偏最小二乘判别分析（OPLS-DA），对柑橘属（Citrus medica L. var. Sarcodactylis Swingle）和枸橘属（C. medica L.）进行了化学分类学研究。结果与目前对 C. medica var.对柑橘果实（CSF）和柑橘果实（CF）的分析表明，除含量外，它们的化学成分高度相似；橙皮甙（HP）和 5，7-二甲氧基香豆素（DMC）是区分柑橘变种（C. medica var.此外，CSF 中 HP 与 DMC 的含量比小于 1（0.2401-0.7953），而 CF 中则大于 1（2.5775-25.7800）。本研究揭示了 HP 和 DMC 对 C. medica var.
Hesperidin and 5, 7-dimethoxycoumarin act as markers in the chemotaxonomy of Citrus medica L. var. sarcodactylis Swingle and Citrus medica L.
Citrus medica L. var. Sarcodactylis Swingle and C. medica L. were the subject of a chemotaxonomic inquiry using high-performance liquid chromatography (HPLC) fingerprint and multivariate data analysis, including hierarchical cluster analysis (HCA), principal component analysis (PCA), and orthogonal partial least squares-discriminant analysis (OPLS-DA). The results are in good agreement with current knowledge of the systematics of C. medica var. Sarcodactylis and C. medica. The analysis of Citri Sarcodactylis Fructus (CSF) and Citri Fructus (CF) showed that their chemical composition was highly similar except for the content; hesperidin (HP) and 5, 7-dimethoxycoumarin (DMC) were distinguishing markers for C. medica var. Sarcodactylis and C. medica; CSF had a significantly higher concentration of DMC than CF; additionally, the content ratio of HP to DMC in CSF was less than one (0.2401–0.7953), but in CF, it was more than one (2.5775–25.7800). The present work revealed the chemotaxonomic significance of HP and DMC for C. medica var. Sarcodactylis and C. medica and validated the traditional plant taxonomy regarding the two species at the chemical level.
期刊介绍：
Biochemical Systematics and Ecology is devoted to the publication of original papers and reviews, both submitted and invited, in two subject areas: I) the application of biochemistry to problems relating to systematic biology of organisms (biochemical systematics); II) the role of biochemistry in interactions between organisms or between an organism and its environment (biochemical ecology).
In the Biochemical Systematics subject area, comparative studies of the distribution of (secondary) metabolites within a wider taxon (e.g. genus or family) are welcome. Comparative studies, encompassing multiple accessions of each of the taxa within their distribution are particularly encouraged. Welcome are also studies combining classical chemosystematic studies (such as comparative HPLC-MS or GC-MS investigations) with (macro-) molecular phylogenetic studies. Studies that involve the comparative use of compounds to help differentiate among species such as adulterants or substitutes that illustrate the applied use of chemosystematics are welcome. In contrast, studies solely employing macromolecular phylogenetic techniques (gene sequences, RAPD studies etc.) will be considered out of scope. Discouraged are manuscripts that report known or new compounds from a single source taxon without addressing a systematic hypothesis. Also considered out of scope are studies using outdated and hard to reproduce macromolecular techniques such as RAPDs in combination with standard chemosystematic techniques such as GC-FID and GC-MS.
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