{"title":"大蒜素影响旋毛虫对核型多角体病毒的易感性","authors":"Jin-Yan Wang, Jie-Xian Jiang, Neng-Neng Fan, Hua-Wu Wu, Xiang-Yun Ji, Nian-Feng Wan, You-Ming Hou","doi":"10.1007/s10340-024-01830-w","DOIUrl":null,"url":null,"abstract":"<p>Plant secondary metabolites are crucial in affecting the interactions between insect herbivores and entomoviruses. However, there is limited knowledge regarding the impact of such metabolites on the susceptibility of insect herbivores to entomoviruses. In this study, we adopted the allicin, caterpillars (<i>Spodoptera exigua</i>) and nucleopolyhedrovirus (SeMNPV) as a system, and found that allicin significantly increased the mortality of <i>S. exigua</i> larvae infected with SeMNPV by 36.03–59.45% when infected with the virus at a concentration of 2.12 × 10<sup>3</sup> OB·mL<sup>−1</sup>. Furthermore, NPV-infected larvae together treated with allicin inhibited the growth and development of larvae, comparing to individual NPV-infected larvae. Notably, we observed a significant enrichment of differentially expressed genes involved in the cytochrome P450-mediated metabolism pathway between the NPV-infected and allicin combined with NPV-treated groups. The silencing of CYP340AA1 through RNA interference significantly increased the mortality of larvae infected with SeMNPV. This investigation indicates that allicin might be a potential candidate for improving the performance of the NPV against insect herbivores and identifies that CYP340AA1 gene is important in this process.</p>","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"5 1","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Allicin impacts the susceptibility of Spodoptera exigua to nucleopolyhedrovirus\",\"authors\":\"Jin-Yan Wang, Jie-Xian Jiang, Neng-Neng Fan, Hua-Wu Wu, Xiang-Yun Ji, Nian-Feng Wan, You-Ming Hou\",\"doi\":\"10.1007/s10340-024-01830-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Plant secondary metabolites are crucial in affecting the interactions between insect herbivores and entomoviruses. However, there is limited knowledge regarding the impact of such metabolites on the susceptibility of insect herbivores to entomoviruses. In this study, we adopted the allicin, caterpillars (<i>Spodoptera exigua</i>) and nucleopolyhedrovirus (SeMNPV) as a system, and found that allicin significantly increased the mortality of <i>S. exigua</i> larvae infected with SeMNPV by 36.03–59.45% when infected with the virus at a concentration of 2.12 × 10<sup>3</sup> OB·mL<sup>−1</sup>. Furthermore, NPV-infected larvae together treated with allicin inhibited the growth and development of larvae, comparing to individual NPV-infected larvae. Notably, we observed a significant enrichment of differentially expressed genes involved in the cytochrome P450-mediated metabolism pathway between the NPV-infected and allicin combined with NPV-treated groups. The silencing of CYP340AA1 through RNA interference significantly increased the mortality of larvae infected with SeMNPV. This investigation indicates that allicin might be a potential candidate for improving the performance of the NPV against insect herbivores and identifies that CYP340AA1 gene is important in this process.</p>\",\"PeriodicalId\":16736,\"journal\":{\"name\":\"Journal of Pest Science\",\"volume\":\"5 1\",\"pages\":\"\"},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-09-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Pest Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s10340-024-01830-w\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Pest Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s10340-024-01830-w","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Allicin impacts the susceptibility of Spodoptera exigua to nucleopolyhedrovirus
Plant secondary metabolites are crucial in affecting the interactions between insect herbivores and entomoviruses. However, there is limited knowledge regarding the impact of such metabolites on the susceptibility of insect herbivores to entomoviruses. In this study, we adopted the allicin, caterpillars (Spodoptera exigua) and nucleopolyhedrovirus (SeMNPV) as a system, and found that allicin significantly increased the mortality of S. exigua larvae infected with SeMNPV by 36.03–59.45% when infected with the virus at a concentration of 2.12 × 103 OB·mL−1. Furthermore, NPV-infected larvae together treated with allicin inhibited the growth and development of larvae, comparing to individual NPV-infected larvae. Notably, we observed a significant enrichment of differentially expressed genes involved in the cytochrome P450-mediated metabolism pathway between the NPV-infected and allicin combined with NPV-treated groups. The silencing of CYP340AA1 through RNA interference significantly increased the mortality of larvae infected with SeMNPV. This investigation indicates that allicin might be a potential candidate for improving the performance of the NPV against insect herbivores and identifies that CYP340AA1 gene is important in this process.
期刊介绍:
Journal of Pest Science publishes high-quality papers on all aspects of pest science in agriculture, horticulture (including viticulture), forestry, urban pests, and stored products research, including health and safety issues.
Journal of Pest Science reports on advances in control of pests and animal vectors of diseases, the biology, ethology and ecology of pests and their antagonists, and the use of other beneficial organisms in pest control. The journal covers all noxious or damaging groups of animals, including arthropods, nematodes, molluscs, and vertebrates.
Journal of Pest Science devotes special attention to emerging and innovative pest control strategies, including the side effects of such approaches on non-target organisms, for example natural enemies and pollinators, and the implementation of these strategies in integrated pest management.
Journal of Pest Science also publishes papers on the management of agro- and forest ecosystems where this is relevant to pest control. Papers on important methodological developments relevant for pest control will be considered as well.