Fengnian Wu, Zehan Dai, Min Shi, Jianjian Huang, Hui Zhu, Yuzhong Zheng, Zikai Chen, Xiuhong Li, Xiaoling Deng, Eduardo G. P. Fox
{"title":"利用有丝分裂基因组和内生共生体追踪亚洲柑橘木虱在中国各地的地理分布情况","authors":"Fengnian Wu, Zehan Dai, Min Shi, Jianjian Huang, Hui Zhu, Yuzhong Zheng, Zikai Chen, Xiuhong Li, Xiaoling Deng, Eduardo G. P. Fox","doi":"10.1007/s10340-024-01834-6","DOIUrl":null,"url":null,"abstract":"<p><i>Diaphorina citri</i> is the vector of “<i>Candidatus</i> Liberibacter asiaticus” (CLas), a bacterium associated with the citrus disease known as Huanglongbing (HLB). Previous mitochondrial genome (i.e. mitogenome) population analyses revealed the prevalence of two major mitochondrial groups (MGs) of <i>D. citri</i> in China, separated by elevation gradients. We assessed the population diversity of <i>D. citri</i> from 54 major citrus-producing areas within 11 provinces/regions of China. Additionally, endosymbiont genomes were assembled for “<i>Ca.</i> Carsonella ruddii” (<i>Ca</i>Cr) and “<i>Ca.</i> Profftella armatura” (<i>Ca</i>Pa) from next-generation sequencing of 31 new Chinese samples. Most of the <i>D. citri</i> diversity came from single nucleotide polymorphisms (SNPs) within five mitochondrial genes: <i>nad3</i>, <i>cox2</i>, <i>rrnL</i>, <i>cob</i>, and <i>atp6</i>. Nine SNPs clustered the analyzed <i>D. citri</i> mitogenomes into three major MGs comprising seven subgroups. Independent phylogenetic trees were generated for the endosymbionts <i>Ca</i>Cr and <i>Ca</i>Pa, and a <i>Ca</i>Pa plasmid, supporting the patterns obtained for <i>D. citri</i> mitogenomes while adding complexity layers. Genomic data from <i>Ca</i>Cr, <i>Ca</i>Pa, along with the plasmids from <i>Ca</i>Pa contribute to the genetic diversity—consisting of 68 SNPs and two genomic gaps—reproducing phylogenetic structures outlined by previous mitochondrial genomic studies. Additionally, both the mitogenomes and the endosymbiont genomes revealed subgroups within the original MG clusters, based on further 154 SNPs and 17 gaps. Thus, the combined genomic approach reveals further aspects about population diversity and natural history of this invasive species. Further understanding of <i>D. citri</i> and its endosymbionts can, therefore, aid <i>D. citri</i> HLB management protocols and help forecast territorial expansion events.</p>","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"5 1","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Tracking the geographical distribution of the Asian citrus psyllid Diaphorina citri throughout China using mitogenomes and endosymbionts\",\"authors\":\"Fengnian Wu, Zehan Dai, Min Shi, Jianjian Huang, Hui Zhu, Yuzhong Zheng, Zikai Chen, Xiuhong Li, Xiaoling Deng, Eduardo G. P. Fox\",\"doi\":\"10.1007/s10340-024-01834-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><i>Diaphorina citri</i> is the vector of “<i>Candidatus</i> Liberibacter asiaticus” (CLas), a bacterium associated with the citrus disease known as Huanglongbing (HLB). Previous mitochondrial genome (i.e. mitogenome) population analyses revealed the prevalence of two major mitochondrial groups (MGs) of <i>D. citri</i> in China, separated by elevation gradients. We assessed the population diversity of <i>D. citri</i> from 54 major citrus-producing areas within 11 provinces/regions of China. Additionally, endosymbiont genomes were assembled for “<i>Ca.</i> Carsonella ruddii” (<i>Ca</i>Cr) and “<i>Ca.</i> Profftella armatura” (<i>Ca</i>Pa) from next-generation sequencing of 31 new Chinese samples. Most of the <i>D. citri</i> diversity came from single nucleotide polymorphisms (SNPs) within five mitochondrial genes: <i>nad3</i>, <i>cox2</i>, <i>rrnL</i>, <i>cob</i>, and <i>atp6</i>. Nine SNPs clustered the analyzed <i>D. citri</i> mitogenomes into three major MGs comprising seven subgroups. Independent phylogenetic trees were generated for the endosymbionts <i>Ca</i>Cr and <i>Ca</i>Pa, and a <i>Ca</i>Pa plasmid, supporting the patterns obtained for <i>D. citri</i> mitogenomes while adding complexity layers. Genomic data from <i>Ca</i>Cr, <i>Ca</i>Pa, along with the plasmids from <i>Ca</i>Pa contribute to the genetic diversity—consisting of 68 SNPs and two genomic gaps—reproducing phylogenetic structures outlined by previous mitochondrial genomic studies. Additionally, both the mitogenomes and the endosymbiont genomes revealed subgroups within the original MG clusters, based on further 154 SNPs and 17 gaps. Thus, the combined genomic approach reveals further aspects about population diversity and natural history of this invasive species. Further understanding of <i>D. citri</i> and its endosymbionts can, therefore, aid <i>D. citri</i> HLB management protocols and help forecast territorial expansion events.</p>\",\"PeriodicalId\":16736,\"journal\":{\"name\":\"Journal of Pest Science\",\"volume\":\"5 1\",\"pages\":\"\"},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-09-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Pest Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s10340-024-01834-6\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Pest Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s10340-024-01834-6","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Tracking the geographical distribution of the Asian citrus psyllid Diaphorina citri throughout China using mitogenomes and endosymbionts
Diaphorina citri is the vector of “Candidatus Liberibacter asiaticus” (CLas), a bacterium associated with the citrus disease known as Huanglongbing (HLB). Previous mitochondrial genome (i.e. mitogenome) population analyses revealed the prevalence of two major mitochondrial groups (MGs) of D. citri in China, separated by elevation gradients. We assessed the population diversity of D. citri from 54 major citrus-producing areas within 11 provinces/regions of China. Additionally, endosymbiont genomes were assembled for “Ca. Carsonella ruddii” (CaCr) and “Ca. Profftella armatura” (CaPa) from next-generation sequencing of 31 new Chinese samples. Most of the D. citri diversity came from single nucleotide polymorphisms (SNPs) within five mitochondrial genes: nad3, cox2, rrnL, cob, and atp6. Nine SNPs clustered the analyzed D. citri mitogenomes into three major MGs comprising seven subgroups. Independent phylogenetic trees were generated for the endosymbionts CaCr and CaPa, and a CaPa plasmid, supporting the patterns obtained for D. citri mitogenomes while adding complexity layers. Genomic data from CaCr, CaPa, along with the plasmids from CaPa contribute to the genetic diversity—consisting of 68 SNPs and two genomic gaps—reproducing phylogenetic structures outlined by previous mitochondrial genomic studies. Additionally, both the mitogenomes and the endosymbiont genomes revealed subgroups within the original MG clusters, based on further 154 SNPs and 17 gaps. Thus, the combined genomic approach reveals further aspects about population diversity and natural history of this invasive species. Further understanding of D. citri and its endosymbionts can, therefore, aid D. citri HLB management protocols and help forecast territorial expansion events.
期刊介绍:
Journal of Pest Science publishes high-quality papers on all aspects of pest science in agriculture, horticulture (including viticulture), forestry, urban pests, and stored products research, including health and safety issues.
Journal of Pest Science reports on advances in control of pests and animal vectors of diseases, the biology, ethology and ecology of pests and their antagonists, and the use of other beneficial organisms in pest control. The journal covers all noxious or damaging groups of animals, including arthropods, nematodes, molluscs, and vertebrates.
Journal of Pest Science devotes special attention to emerging and innovative pest control strategies, including the side effects of such approaches on non-target organisms, for example natural enemies and pollinators, and the implementation of these strategies in integrated pest management.
Journal of Pest Science also publishes papers on the management of agro- and forest ecosystems where this is relevant to pest control. Papers on important methodological developments relevant for pest control will be considered as well.