Nan Wang MSc , Qianzi Ma MSc , Jiayuan Zhai MSc , Yanlu Che MSc , Junjie Liu MSc , Tianwei Tang MSc , Yanan Sun PhD , Jingting Wang PhD , Wanchao Yang PhD
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This study aimed to explore the possible mechanism of action of H<sub>2</sub> on AR through its ability to regulate the balance of nasal flora.</p></div><div><h3>Methods</h3><p>Serum eosinophil count (EOS), immunoglobulin E (IgE) concentration, visual analog scale (VAS), total nasal symptom score (TNSS), and rhinoconjunctivitis quality of life questionnaire (RQLQ) were observed before and after hydrogen inhalation in AR patients. Skin prick test (SPT) was used to determine allergen sensitisation. Community composition and relative abundance of nasal flora were examined before and after hydrogen inhalation and in normal subjects using 16S rRNA gene sequencing.</p></div><div><h3>Results</h3><p>There were no adverse reactions during and after hydrogen inhalation in AR patients, with a favorable safety profile and significant improvements in VAS, TNSS, EOS, and IgE (P < 0.05). Cavity flora 16S rRNA gene sequencing showed higher abundance of Ruminococcus and Erysipelotrichaceae flora in the nasal cavity of AR patients than in normal subjects, and their abundance could be down-regulated after H<sub>2</sub> inhalation. H<sub>2</sub> significantly increased the abundance of Blautia_faecis and negatively correlated with VAS, TNSS, EOS, and IgE.</p></div><div><h3>Conclusions</h3><p>H<sub>2</sub> may improve symptoms in AR patients by modulating the distribution of nasal flora. Trials with larger sample sizes are required to further test this hypothesis.</p></div><div><h3>Trial registration</h3><p>This trial was registered in the China Clinical Trial Registry (Registration No. ChiCTR2200062253).</p></div>","PeriodicalId":54295,"journal":{"name":"World Allergy Organization Journal","volume":"17 10","pages":"Article 100970"},"PeriodicalIF":3.9000,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1939455124001029/pdfft?md5=6057376b9ebb8c62876dc1de4c1e1586&pid=1-s2.0-S1939455124001029-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Hydrogen inhalation: A novel approach to alleviating allergic rhinitis symptoms by modulating nasal flora\",\"authors\":\"Nan Wang MSc , Qianzi Ma MSc , Jiayuan Zhai MSc , Yanlu Che MSc , Junjie Liu MSc , Tianwei Tang MSc , Yanan Sun PhD , Jingting Wang PhD , Wanchao Yang PhD\",\"doi\":\"10.1016/j.waojou.2024.100970\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Allergic rhinitis (AR) is an allergic reaction dominated by the Th2 immune response in the nasal mucosa. The bacterial infection process affects the balance between Th1 and Th2 immune responses, and the level of exposure to environmental flora is closely related to the development of AR. Hydrogen (H<sub>2</sub>) is a medical molecule with anti-inflammatory and antioxidant properties. This study aimed to explore the possible mechanism of action of H<sub>2</sub> on AR through its ability to regulate the balance of nasal flora.</p></div><div><h3>Methods</h3><p>Serum eosinophil count (EOS), immunoglobulin E (IgE) concentration, visual analog scale (VAS), total nasal symptom score (TNSS), and rhinoconjunctivitis quality of life questionnaire (RQLQ) were observed before and after hydrogen inhalation in AR patients. Skin prick test (SPT) was used to determine allergen sensitisation. Community composition and relative abundance of nasal flora were examined before and after hydrogen inhalation and in normal subjects using 16S rRNA gene sequencing.</p></div><div><h3>Results</h3><p>There were no adverse reactions during and after hydrogen inhalation in AR patients, with a favorable safety profile and significant improvements in VAS, TNSS, EOS, and IgE (P < 0.05). Cavity flora 16S rRNA gene sequencing showed higher abundance of Ruminococcus and Erysipelotrichaceae flora in the nasal cavity of AR patients than in normal subjects, and their abundance could be down-regulated after H<sub>2</sub> inhalation. H<sub>2</sub> significantly increased the abundance of Blautia_faecis and negatively correlated with VAS, TNSS, EOS, and IgE.</p></div><div><h3>Conclusions</h3><p>H<sub>2</sub> may improve symptoms in AR patients by modulating the distribution of nasal flora. 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引用次数: 0
摘要
背景过敏性鼻炎（AR）是一种以鼻粘膜 Th2 免疫反应为主的过敏反应。细菌感染过程会影响 Th1 和 Th2 免疫反应之间的平衡，环境菌群的暴露水平与 AR 的发生密切相关。氢气（H2）是一种具有抗炎和抗氧化特性的医用分子。本研究旨在探讨氢气通过调节鼻腔菌群平衡对 AR 的可能作用机制。方法观察 AR 患者吸入氢气前后的血清嗜酸性粒细胞计数（EOS）、免疫球蛋白 E（IgE）浓度、视觉模拟量表（VAS）、鼻腔症状总评分（TNSS）和鼻结膜炎生活质量问卷（RQLQ）。皮肤点刺试验（SPT）用于确定过敏原致敏性。结果AR 患者在吸入氢气前后均未出现不良反应，安全性良好，VAS、TNSS、EOS 和 IgE 均有显著改善（P < 0.05）。腔内菌群 16S rRNA 基因测序显示，AR 患者鼻腔内的反刍球菌和绿脓杆菌菌群丰度高于正常人，吸入氢气后它们的丰度可被下调。结论 H2 可通过调节鼻腔菌群的分布来改善 AR 患者的症状。本试验已在中国临床试验注册中心注册（注册号：ChiCTR2200062253）。
Hydrogen inhalation: A novel approach to alleviating allergic rhinitis symptoms by modulating nasal flora
Background
Allergic rhinitis (AR) is an allergic reaction dominated by the Th2 immune response in the nasal mucosa. The bacterial infection process affects the balance between Th1 and Th2 immune responses, and the level of exposure to environmental flora is closely related to the development of AR. Hydrogen (H2) is a medical molecule with anti-inflammatory and antioxidant properties. This study aimed to explore the possible mechanism of action of H2 on AR through its ability to regulate the balance of nasal flora.
Methods
Serum eosinophil count (EOS), immunoglobulin E (IgE) concentration, visual analog scale (VAS), total nasal symptom score (TNSS), and rhinoconjunctivitis quality of life questionnaire (RQLQ) were observed before and after hydrogen inhalation in AR patients. Skin prick test (SPT) was used to determine allergen sensitisation. Community composition and relative abundance of nasal flora were examined before and after hydrogen inhalation and in normal subjects using 16S rRNA gene sequencing.
Results
There were no adverse reactions during and after hydrogen inhalation in AR patients, with a favorable safety profile and significant improvements in VAS, TNSS, EOS, and IgE (P < 0.05). Cavity flora 16S rRNA gene sequencing showed higher abundance of Ruminococcus and Erysipelotrichaceae flora in the nasal cavity of AR patients than in normal subjects, and their abundance could be down-regulated after H2 inhalation. H2 significantly increased the abundance of Blautia_faecis and negatively correlated with VAS, TNSS, EOS, and IgE.
Conclusions
H2 may improve symptoms in AR patients by modulating the distribution of nasal flora. Trials with larger sample sizes are required to further test this hypothesis.
Trial registration
This trial was registered in the China Clinical Trial Registry (Registration No. ChiCTR2200062253).
期刊介绍：
The official pubication of the World Allergy Organization, the World Allergy Organization Journal (WAOjournal) publishes original mechanistic, translational, and clinical research on the topics of allergy, asthma, anaphylaxis, and clincial immunology, as well as reviews, guidelines, and position papers that contribute to the improvement of patient care. WAOjournal publishes research on the growth of allergy prevalence within the scope of single countries, country comparisons, and practical global issues and regulations, or threats to the allergy specialty. The Journal invites the submissions of all authors interested in publishing on current global problems in allergy, asthma, anaphylaxis, and immunology. Of particular interest are the immunological consequences of climate change and the subsequent systematic transformations in food habits and their consequences for the allergy/immunology discipline.
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