{"title":"化学和生物战剂去污剂对痕量存活的影响:对血液和唾液 DNA 分析的影响","authors":"","doi":"10.1016/j.forsciint.2024.112206","DOIUrl":null,"url":null,"abstract":"<div><p>Forensic investigations following incidents involving chemical or biological agents present considerable challenges. Understanding the possibilities and limitations can aid in determining the most suitable procedures and enhancing the recovery of useful traces in these complex situations. This work complements previously published results on the effects of decontaminants on fingermarks deposited on glass. Identifying the perpetrators can be crucial, and DNA analysis remains a cornerstone in this regard. In this study, we investigated the ability to obtain usable DNA profiles from blood and saliva (pure and diluted) exposed to 16 different decontamination methods. Both DNA quantitation and DNA profiling were considered to assess the outcomes. The results revealed considerable variability but indicated that biological agents' decontaminants hindered DNA profiling post-decontamination to a greater extent than decontaminants aimed for chemical agents. Chlorine-based decontaminants also globally had a deleterious impact on DNA profiling. Powder decontaminants such as Fast-Act, CHpowder, and the liquid decontaminants GDS2000 did not affect DNA profiling.</p></div>","PeriodicalId":12341,"journal":{"name":"Forensic science international","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0379073824002871/pdfft?md5=7a8c4e6b0db979217bb0b4d37ddc9a0f&pid=1-s2.0-S0379073824002871-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Effects of chemical & biological warfare agent decontaminants on trace survival: Impact on DNA profiling from blood and saliva\",\"authors\":\"\",\"doi\":\"10.1016/j.forsciint.2024.112206\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Forensic investigations following incidents involving chemical or biological agents present considerable challenges. Understanding the possibilities and limitations can aid in determining the most suitable procedures and enhancing the recovery of useful traces in these complex situations. This work complements previously published results on the effects of decontaminants on fingermarks deposited on glass. Identifying the perpetrators can be crucial, and DNA analysis remains a cornerstone in this regard. In this study, we investigated the ability to obtain usable DNA profiles from blood and saliva (pure and diluted) exposed to 16 different decontamination methods. Both DNA quantitation and DNA profiling were considered to assess the outcomes. The results revealed considerable variability but indicated that biological agents' decontaminants hindered DNA profiling post-decontamination to a greater extent than decontaminants aimed for chemical agents. Chlorine-based decontaminants also globally had a deleterious impact on DNA profiling. Powder decontaminants such as Fast-Act, CHpowder, and the liquid decontaminants GDS2000 did not affect DNA profiling.</p></div>\",\"PeriodicalId\":12341,\"journal\":{\"name\":\"Forensic science international\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-09-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0379073824002871/pdfft?md5=7a8c4e6b0db979217bb0b4d37ddc9a0f&pid=1-s2.0-S0379073824002871-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Forensic science international\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0379073824002871\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MEDICINE, LEGAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Forensic science international","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0379073824002871","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MEDICINE, LEGAL","Score":null,"Total":0}
引用次数: 0
摘要
在涉及化学或生物制剂的事件发生后进行法医调查是一项相当大的挑战。了解其可能性和局限性有助于确定最合适的程序,并提高在这些复杂情况下恢复有用痕迹的能力。这项工作补充了之前发表的关于去污剂对沉积在玻璃上的指痕的影响的研究成果。识别罪犯可能至关重要,而 DNA 分析仍然是这方面的基石。在这项研究中,我们调查了从暴露于 16 种不同去污方法的血液和唾液(纯净和稀释)中获取可用 DNA 图谱的能力。在评估结果时,我们同时考虑了 DNA 定量和 DNA 图谱分析。结果表明,生物制剂去污剂与化学制剂去污剂相比,在很大程度上妨碍了去污后的 DNA 图谱分析。氯基洗消剂在全球范围内也对 DNA 分析产生了有害影响。粉末去污剂(如 Fast-Act、CHpowder)和液体去污剂 GDS2000 对 DNA 分析没有影响。
Effects of chemical & biological warfare agent decontaminants on trace survival: Impact on DNA profiling from blood and saliva
Forensic investigations following incidents involving chemical or biological agents present considerable challenges. Understanding the possibilities and limitations can aid in determining the most suitable procedures and enhancing the recovery of useful traces in these complex situations. This work complements previously published results on the effects of decontaminants on fingermarks deposited on glass. Identifying the perpetrators can be crucial, and DNA analysis remains a cornerstone in this regard. In this study, we investigated the ability to obtain usable DNA profiles from blood and saliva (pure and diluted) exposed to 16 different decontamination methods. Both DNA quantitation and DNA profiling were considered to assess the outcomes. The results revealed considerable variability but indicated that biological agents' decontaminants hindered DNA profiling post-decontamination to a greater extent than decontaminants aimed for chemical agents. Chlorine-based decontaminants also globally had a deleterious impact on DNA profiling. Powder decontaminants such as Fast-Act, CHpowder, and the liquid decontaminants GDS2000 did not affect DNA profiling.
期刊介绍:
Forensic Science International is the flagship journal in the prestigious Forensic Science International family, publishing the most innovative, cutting-edge, and influential contributions across the forensic sciences. Fields include: forensic pathology and histochemistry, chemistry, biochemistry and toxicology, biology, serology, odontology, psychiatry, anthropology, digital forensics, the physical sciences, firearms, and document examination, as well as investigations of value to public health in its broadest sense, and the important marginal area where science and medicine interact with the law.
The journal publishes:
Case Reports
Commentaries
Letters to the Editor
Original Research Papers (Regular Papers)
Rapid Communications
Review Articles
Technical Notes.