灵芝中的 3,4-seco-三萜类抗炎物质

IF 2.1 3区化学 Q2 CHEMISTRY, ORGANIC

Tetrahedron Pub Date : 2024-09-06 DOI:10.1016/j.tet.2024.134240

Zhi-Yuan Li , Jian-Biao Zhang , Yong-Xian Cheng

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引用次数: 0

摘要

研究人员从灵芝中分离出了五种新的 3,4-seco-lanostane三萜类化合物，命名为灵芝素 A-E（1-5），以及三种已知的类似物（6-8）。通过一维和二维核磁共振（NMR）、计算方法和 HRESIMS 对这些新化合物的结构（包括其绝对构型）进行了表征。其中，化合物 1 的结构通过 X 射线晶体学分析得到了证实。在 LPS 诱导的 RAW264.7 细胞中评估了所有化合物的抗炎活性。Western 印迹分析表明，化合物 4 和 5 对 LPS 诱导的 RAW264.7 细胞中 iNOS 蛋白表达以及 TNF-a、Il-1b 和 Il-6 的 mRNA 水平有抑制作用，化合物 4 还能降低 COX-2 蛋白表达。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Anti-inflammatory 3,4-seco-triterpenoids from Ganoderma cochlear

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Anti-inflammatory 3,4-seco-triterpenoids from Ganoderma cochlear

Five new 3,4-seco-lanostane triterpenoids, named ganocochnoids A−E (1−5), along with three known analogues (6−8), were isolated from Ganoderma cochlear. The structures of these new compounds, including their absolute configurations, were characterized by 1D and 2D nuclear magnetic resonance (NMR), computational methods, and HRESIMS. In particular, the structure of compound 1 was confirmed through X-ray crystallographic analysis. The anti-inflammatory activity of all the compounds was evaluated in LPS-induced RAW264.7 cells. The Western blot assay indicated that compounds 4 and 5 were found to have inhibitory effects on iNOS protein expression as well as on mRNA levels of TNF-a, Il-1b, and Il-6, and compound 4 also reduced COX-2 protein expression in LPS-induced RAW264.7 cells.

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来源期刊

Tetrahedron 化学-有机化学

CiteScore

3.90

自引率

4.80%

发文量

439

审稿时长

34 days

期刊介绍： Tetrahedron publishes full accounts of research having outstanding significance in the broad field of organic chemistry and its related disciplines, such as organic materials and bio-organic chemistry. Regular papers in Tetrahedron are expected to represent detailed accounts of an original study having substantially greater scope and details than that found in a communication, as published in Tetrahedron Letters. Tetrahedron also publishes thematic collections of papers as special issues and ''Reports'', commissioned in-depth reviews providing a comprehensive overview of a research area.