Jichang Song , Yige Li , Ziyang Zhang , Xinlong Gao , Shengxue Li , Jie Zhang , Mingguo Zhou , Yabing Duan
{"title":"内质网-线粒体相遇结构调控禾谷镰刀菌的线粒体形态、DON 生物合成和毒素组形成","authors":"Jichang Song , Yige Li , Ziyang Zhang , Xinlong Gao , Shengxue Li , Jie Zhang , Mingguo Zhou , Yabing Duan","doi":"10.1016/j.micres.2024.127892","DOIUrl":null,"url":null,"abstract":"<div><p>The endoplasmic reticulum-mitochondrial encounter structure (ERMES) complex is known to play crucial roles in various cellular processes. However, its functional significance in filamentous fungi, particularly its impact on deoxynivalenol (DON) biosynthesis in <em>Fusarium graminearum</em>, remains inadequately understood. In this study, we aimed to investigate the regulatory function of the ERMES complex in <em>F. graminearum</em>. Our findings indicate significant changes in mitochondrial morphology of ERMES mutants, accompanied by decreased ATP content and ergosterol production. Notably, the toxisome formation in the ERMES mutant ΔFgMDM10 was defective, resulting in a substantial reduction in DON biosynthesis. This suggests a pivotal role of ERMES in toxisome formation, as evidenced by the pronounced inhibition of toxisome formation when ERMES was disrupted by boscalid. Furthermore, ERMES deficiencies were shown to diminish the virulence of <em>F. graminearum</em> towards host plants significantly. In conclusion, our results suggest ERMES is an important regulator of mitochondrial morphology, DON biosynthesis, and toxisome formation in <em>F. graminearum</em>.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.1000,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0944501324002933/pdfft?md5=47dc1cf725902f89c167c2c6bdd688eb&pid=1-s2.0-S0944501324002933-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Endoplasmic reticulum-mitochondrial encounter structure regulates the mitochondrial morphology, DON biosynthesis and toxisome formation in Fusarium graminearum\",\"authors\":\"Jichang Song , Yige Li , Ziyang Zhang , Xinlong Gao , Shengxue Li , Jie Zhang , Mingguo Zhou , Yabing Duan\",\"doi\":\"10.1016/j.micres.2024.127892\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The endoplasmic reticulum-mitochondrial encounter structure (ERMES) complex is known to play crucial roles in various cellular processes. However, its functional significance in filamentous fungi, particularly its impact on deoxynivalenol (DON) biosynthesis in <em>Fusarium graminearum</em>, remains inadequately understood. In this study, we aimed to investigate the regulatory function of the ERMES complex in <em>F. graminearum</em>. Our findings indicate significant changes in mitochondrial morphology of ERMES mutants, accompanied by decreased ATP content and ergosterol production. Notably, the toxisome formation in the ERMES mutant ΔFgMDM10 was defective, resulting in a substantial reduction in DON biosynthesis. This suggests a pivotal role of ERMES in toxisome formation, as evidenced by the pronounced inhibition of toxisome formation when ERMES was disrupted by boscalid. Furthermore, ERMES deficiencies were shown to diminish the virulence of <em>F. graminearum</em> towards host plants significantly. In conclusion, our results suggest ERMES is an important regulator of mitochondrial morphology, DON biosynthesis, and toxisome formation in <em>F. graminearum</em>.</p></div>\",\"PeriodicalId\":18564,\"journal\":{\"name\":\"Microbiological research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":6.1000,\"publicationDate\":\"2024-09-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0944501324002933/pdfft?md5=47dc1cf725902f89c167c2c6bdd688eb&pid=1-s2.0-S0944501324002933-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbiological research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0944501324002933\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiological research","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0944501324002933","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
众所周知,内质网-线粒体相遇结构(ERMES)复合物在各种细胞过程中发挥着至关重要的作用。然而,人们对它在丝状真菌中的功能意义,特别是它对禾谷镰刀菌中脱氧雪腐镰刀菌醇(DON)生物合成的影响,仍然缺乏足够的了解。在本研究中,我们旨在研究ERMES复合体在禾谷镰刀菌中的调控功能。我们的研究结果表明,ERMES 突变体的线粒体形态发生了显著变化,同时 ATP 含量和麦角甾醇产量也有所下降。值得注意的是,ERMES 突变体 ΔFgMDM10 的毒素体形成存在缺陷,导致 DON 生物合成大幅减少。这表明ERMES在毒素体形成过程中起着关键作用,当ERMES被boscalid破坏时,毒素体的形成受到明显抑制就是证明。此外,ERMES 的缺失还能显著降低禾本科镰刀菌对寄主植物的毒力。总之,我们的研究结果表明,ERMES 是禾谷镰孢线粒体形态、DON 生物合成和毒素体形成的重要调节因子。
Endoplasmic reticulum-mitochondrial encounter structure regulates the mitochondrial morphology, DON biosynthesis and toxisome formation in Fusarium graminearum
The endoplasmic reticulum-mitochondrial encounter structure (ERMES) complex is known to play crucial roles in various cellular processes. However, its functional significance in filamentous fungi, particularly its impact on deoxynivalenol (DON) biosynthesis in Fusarium graminearum, remains inadequately understood. In this study, we aimed to investigate the regulatory function of the ERMES complex in F. graminearum. Our findings indicate significant changes in mitochondrial morphology of ERMES mutants, accompanied by decreased ATP content and ergosterol production. Notably, the toxisome formation in the ERMES mutant ΔFgMDM10 was defective, resulting in a substantial reduction in DON biosynthesis. This suggests a pivotal role of ERMES in toxisome formation, as evidenced by the pronounced inhibition of toxisome formation when ERMES was disrupted by boscalid. Furthermore, ERMES deficiencies were shown to diminish the virulence of F. graminearum towards host plants significantly. In conclusion, our results suggest ERMES is an important regulator of mitochondrial morphology, DON biosynthesis, and toxisome formation in F. graminearum.
期刊介绍:
Microbiological Research is devoted to publishing reports on prokaryotic and eukaryotic microorganisms such as yeasts, fungi, bacteria, archaea, and protozoa. Research on interactions between pathogenic microorganisms and their environment or hosts are also covered.