短肽和噬菌体衍生内溶酶对抗生素耐药鼠伤寒沙门氏菌的联合抗菌活性

IF 4.5 1区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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引用次数: 0

摘要

本研究旨在评估抗菌肽(FK13 和 FK16)与噬菌体编码的内溶菌素(LysPB32)联合抑制耐多粘菌素 B 的鼠伤寒沙门氏菌 ATCC 19585(STPMB)生长的效果。通过抗菌药敏感性、膜渗透性、生物膜减少、交叉耐药性和突变频率检测,评估了 FK13、FK16 和 LysPB32 对 STPMB 的抑制作用。经 LysPB32 处理的 FK13 和 FK16(FK13+LysPB32 和 FK16+LysPB32)对 STPMB 的最小抑菌浓度(MICs)分别从 512 多微克/毫升降至 128 微克/毫升,从 64 微克/毫升降至 32 微克/毫升。与对照组相比,FK13+LysPB32 和 FK16+LysPB32 在 37 ℃ 培养 12 小时后,生长培养物中的 STPMB 数量分别减少了 4.2 和 5.2 log CFU/ml。所有处理(FK13、FK16、FK13+LysPB32、FK16+LysPB32)都显著增加了 STPMB 外膜的通透性。FK13+LysPB32 可使生物膜的 OD600 从 0.6 显著降至 0.16,FK16+LysPB32 可使生物膜的 OD600 从 0.6 显著降至 0.13。在 FK13+LysPB32 和 FK16+LysPB32 中,红霉素、头孢曲松、多粘菌素 B 和环丙沙星对 STPMB 的 MIC 与对照的 MIC 之比降至 0.50。FK13+LysPB32 和 FK16+LysPB32 可增强阿米卡星和庆大霉素的杀菌活性(MBC/MIC 比为 2 倍)。使用 FK13+LysPB32 和 FK16+LysPB32 时,STPMB 对抗生素的突变频率降低。结果表明,抗菌肽与内溶素的结合是控制耐多粘菌素 B 的鼠伤寒杆菌的一种有效策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Combined antimicrobial activity of short peptide and phage-derived endolysin against antibiotic-resistant Salmonella Typhimurium

Combined antimicrobial activity of short peptide and phage-derived endolysin against antibiotic-resistant Salmonella Typhimurium

This study was designed to evaluate the combination effects of antimicrobial peptides (FK13 and FK16) and phage-encoded endolysin (LysPB32) on the inhibition of growth of polymyxin B-resistant Salmonella Typhimurium ATCC 19585 (STPMB). The inhibitory effects of FK13, FK16, and LysPB32 against STPMB were evaluated by using antimicrobial susceptibility, membrane permeability, biofilm reduction, cross-resistance, and mutant frequency assay. The minimum inhibitory concentrations (MICs) of FK13 and FK16 treated with LysPB32 (FK13+LysPB32 and FK16+LysPB32) against STPMB were decreased from more than 512 to 128 μg/ml and from 64 to 32 μg/ml, respectively. Compared to the control, the number of STPMB in the growing culture was reduced by 4.2 and 5.2 log CFU/ml, respectively, for FK13+LysPB32 and FK16+LysPB32 after 12-h incubation at 37 °C. All treatments (FK13, FK16, FK13+LysPB32, FK16+LysPB32) significantly increased the permeability of the outer membrane of STPMB. Biofilms were significantly decreased from OD600 of 0.6 to 0.16 for FK13+LysPB32 and from 0.6 to 0.13 for FK16+LysPB32. The ratios of MICs of erythromycin, ceftriaxone, polymyxin B, and ciprofloxacin to MIC of the control against STPMB were decreased to 0.50 for FK13+LysPB32 and FK16+LysPB32. The bactericidal activities of amikacin and gentamicin were enhanced for FK13+LysPB32 and FK16+LysPB32 (2-fold < MBC/MIC ratio). The mutant frequencies of STPMB to antibiotics were decreased when treated with FK13+LysPB32 and FK16+LysPB32. The results suggest that the combination of antimicrobial peptides and endolysins can be a promising strategy to control polymyxin B-resistant S. Typhimurium.

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来源期刊
Food microbiology
Food microbiology 工程技术-生物工程与应用微生物
CiteScore
11.30
自引率
3.80%
发文量
179
审稿时长
44 days
期刊介绍: Food Microbiology publishes original research articles, short communications, review papers, letters, news items and book reviews dealing with all aspects of the microbiology of foods. The editors aim to publish manuscripts of the highest quality which are both relevant and applicable to the broad field covered by the journal. Studies must be novel, have a clear connection to food microbiology, and be of general interest to the international community of food microbiologists. The editors make every effort to ensure rapid and fair reviews, resulting in timely publication of accepted manuscripts.
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