{"title":"癌胚抗原生物传感技术的最新进展与挑战","authors":"","doi":"10.1016/j.trac.2024.117964","DOIUrl":null,"url":null,"abstract":"<div><p>Carcinoembryonic antigen (CEA) is a multifunctional glycoprotein that belongs to the immunoglobulin superfamily and plays dual regulatory roles in cancer and fetal development. Due to its crucial biological functions in cancer regulation, CEA levels elevate abnormally in colorectal, breast, lung, gastric, medullary thyroid, and pancreatic cancers. Furthermore, conditions unrelated to neoplasms, such as ulcerative pancreatitis, chronic obstructive pulmonary disease (COPD), cirrhosis, colitis, hypothyroidism, Crohn's disease, and smoking are associated with increased CEA levels. Therefore, developing precise and sensitive strategies for monitoring CEA holds significant importance. This study emphasizes the successful creating of detection methods through surface modification, bio-functionalization, and nano-fabrication to enable effective detection of CEA at trace levels (with a threshold of 20 ng mL<sup>−1</sup>). It explores various optical and electrochemical (EC) sensors, including electrochemiluminescence (ECL), fluorometric, surface-enhanced Raman scattering (SERS), photoelectrochemical (PEC), and colorimetric methods. The study highlights the integrating of innovative nanomaterials with a range of bioreceptor elements, including peptides, aptamers (Apt), DNA, enzymes, and antibodies (Abs), to enhance the effectiveness of CEA tracking and quantification, which are essential for analytical methods. Additionally, the research identifies gaps, challenges, and areas for improvement in CEA biosensing methods, research, and technology. 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Due to its crucial biological functions in cancer regulation, CEA levels elevate abnormally in colorectal, breast, lung, gastric, medullary thyroid, and pancreatic cancers. Furthermore, conditions unrelated to neoplasms, such as ulcerative pancreatitis, chronic obstructive pulmonary disease (COPD), cirrhosis, colitis, hypothyroidism, Crohn's disease, and smoking are associated with increased CEA levels. Therefore, developing precise and sensitive strategies for monitoring CEA holds significant importance. This study emphasizes the successful creating of detection methods through surface modification, bio-functionalization, and nano-fabrication to enable effective detection of CEA at trace levels (with a threshold of 20 ng mL<sup>−1</sup>). It explores various optical and electrochemical (EC) sensors, including electrochemiluminescence (ECL), fluorometric, surface-enhanced Raman scattering (SERS), photoelectrochemical (PEC), and colorimetric methods. 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引用次数: 0
摘要
癌胚抗原(CEA)是一种多功能糖蛋白,属于免疫球蛋白超家族,在癌症和胎儿发育过程中发挥双重调控作用。由于其在癌症调控中的重要生物学功能,CEA 水平在结直肠癌、乳腺癌、肺癌、胃癌、甲状腺髓样癌和胰腺癌中异常升高。此外,溃疡性胰腺炎、慢性阻塞性肺病(COPD)、肝硬化、结肠炎、甲状腺功能减退症、克罗恩病和吸烟等与肿瘤无关的疾病也与 CEA 水平升高有关。因此,开发精确、灵敏的 CEA 监测策略具有重要意义。本研究强调通过表面改性、生物功能化和纳米制造成功创建检测方法,以实现对痕量水平(阈值为 20 纳克毫升-1)的 CEA 的有效检测。研究探讨了各种光学和电化学 (EC) 传感器,包括电化学发光 (ECL)、荧光测定、表面增强拉曼散射 (SERS)、光电化学 (PEC) 和比色法。该研究强调了创新纳米材料与一系列生物受体元素(包括肽、aptamers (Apt)、DNA、酶和抗体 (Abs))的整合,以提高 CEA 跟踪和定量的有效性,这对分析方法至关重要。此外,研究还确定了 CEA 生物传感方法、研究和技术方面的差距、挑战和有待改进的领域。本综述是通过应用生物传感技术对 CEA 跟踪进行的一次前所未有的评估。
Recent progress and challenges in biosensing of carcinoembryonic antigen
Carcinoembryonic antigen (CEA) is a multifunctional glycoprotein that belongs to the immunoglobulin superfamily and plays dual regulatory roles in cancer and fetal development. Due to its crucial biological functions in cancer regulation, CEA levels elevate abnormally in colorectal, breast, lung, gastric, medullary thyroid, and pancreatic cancers. Furthermore, conditions unrelated to neoplasms, such as ulcerative pancreatitis, chronic obstructive pulmonary disease (COPD), cirrhosis, colitis, hypothyroidism, Crohn's disease, and smoking are associated with increased CEA levels. Therefore, developing precise and sensitive strategies for monitoring CEA holds significant importance. This study emphasizes the successful creating of detection methods through surface modification, bio-functionalization, and nano-fabrication to enable effective detection of CEA at trace levels (with a threshold of 20 ng mL−1). It explores various optical and electrochemical (EC) sensors, including electrochemiluminescence (ECL), fluorometric, surface-enhanced Raman scattering (SERS), photoelectrochemical (PEC), and colorimetric methods. The study highlights the integrating of innovative nanomaterials with a range of bioreceptor elements, including peptides, aptamers (Apt), DNA, enzymes, and antibodies (Abs), to enhance the effectiveness of CEA tracking and quantification, which are essential for analytical methods. Additionally, the research identifies gaps, challenges, and areas for improvement in CEA biosensing methods, research, and technology. This review represents an unprecedented assessment of CEA tracking through the application of biosensor technology.
期刊介绍:
TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.