{"title":"纳米微粒引起的叶片解剖结构和细胞壁成分变化推动了盐胁迫棉花叶片叶脉间传导率的增加","authors":"","doi":"10.1016/j.plaphy.2024.109111","DOIUrl":null,"url":null,"abstract":"<div><p>Nanomaterials as an emerging tool are being used to improve plant's net photosynthetic rate (<em>A</em><sub>N</sub>) when suffering salt stress, but the underlying mechanisms remain unclear. To clarify this, a hydroponic experiment was conducted to study the effects of polyacrylic acid coated nanoceria (PNC) on the <em>A</em><sub>N</sub> of salt-stressed cotton and related intrinsic mechanisms. Results showed that the PNC-induced <em>A</em><sub>N</sub> enhancement of salt-stressed leaves was strongly facilitated by the mesophyll conductance to CO<sub>2</sub> (<em>g</em><sub>m</sub>). Further analysis showed that the PNC-induced improvement of <em>g</em><sub>m</sub> was related to the increased chloroplast surface area exposed to intercellular airspaces, which was attribute to the increased mesophyll surface area exposed to intercellular airspaces and chloroplast number due to the increased K<sup>+</sup> content and decreased reactive oxygen species level in salt-stressed leaves. Interestingly, our results also showed that PNC-induced variations in cell wall composition of salt-stressed cotton leaves strongly influenced <em>g</em><sub>m</sub>, especially, hemicellulose and pectin. Moreover, the proportion of pectin in cell wall composition played a more important role in determining <em>g</em><sub>m</sub>. Our study demonstrated for the first time that nanoceria, through alterations to anatomical traits and cell wall composition, drove <em>g</em><sub>m</sub> enhancement, which ultimately increased <em>A</em><sub>N</sub> of salt-stressed leaves.</p></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1000,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Nanoceria-induced variations in leaf anatomy and cell wall composition drive the increase in mesophyll conductance of salt-stressed cotton leaves\",\"authors\":\"\",\"doi\":\"10.1016/j.plaphy.2024.109111\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Nanomaterials as an emerging tool are being used to improve plant's net photosynthetic rate (<em>A</em><sub>N</sub>) when suffering salt stress, but the underlying mechanisms remain unclear. To clarify this, a hydroponic experiment was conducted to study the effects of polyacrylic acid coated nanoceria (PNC) on the <em>A</em><sub>N</sub> of salt-stressed cotton and related intrinsic mechanisms. Results showed that the PNC-induced <em>A</em><sub>N</sub> enhancement of salt-stressed leaves was strongly facilitated by the mesophyll conductance to CO<sub>2</sub> (<em>g</em><sub>m</sub>). Further analysis showed that the PNC-induced improvement of <em>g</em><sub>m</sub> was related to the increased chloroplast surface area exposed to intercellular airspaces, which was attribute to the increased mesophyll surface area exposed to intercellular airspaces and chloroplast number due to the increased K<sup>+</sup> content and decreased reactive oxygen species level in salt-stressed leaves. Interestingly, our results also showed that PNC-induced variations in cell wall composition of salt-stressed cotton leaves strongly influenced <em>g</em><sub>m</sub>, especially, hemicellulose and pectin. Moreover, the proportion of pectin in cell wall composition played a more important role in determining <em>g</em><sub>m</sub>. Our study demonstrated for the first time that nanoceria, through alterations to anatomical traits and cell wall composition, drove <em>g</em><sub>m</sub> enhancement, which ultimately increased <em>A</em><sub>N</sub> of salt-stressed leaves.</p></div>\",\"PeriodicalId\":20234,\"journal\":{\"name\":\"Plant Physiology and Biochemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":6.1000,\"publicationDate\":\"2024-09-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Physiology and Biochemistry\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0981942824007794\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0981942824007794","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
纳米材料作为一种新兴工具,正被用于提高植物在遭受盐胁迫时的净光合速率(AN),但其内在机制仍不清楚。为了澄清这一问题,我们进行了一项水培实验,研究聚丙烯酸包覆纳米铈(PNC)对盐胁迫棉花净光合速率的影响及相关内在机制。结果表明,PNC 诱导的盐胁迫叶片 AN 增强在很大程度上受叶绿体对 CO2 的传导(gm)的影响。进一步的分析表明,PNC 诱导的 gm 提高与暴露于细胞间空隙的叶绿体表面积增加有关,而叶绿体表面积增加又归因于盐胁迫叶片中 K+ 含量增加和活性氧水平降低导致暴露于细胞间空隙的叶绿体表面积和叶绿体数量增加。有趣的是,我们的研究结果还表明,PNC 诱导的盐胁迫棉叶细胞壁成分变化对 gm 有很大影响,尤其是半纤维素和果胶。此外,果胶在细胞壁组成中所占的比例在决定棉重方面起着更加重要的作用。我们的研究首次证明,纳米微粒通过改变解剖学特征和细胞壁组成,促进了棉花植株长势,最终提高了盐胁迫叶片的抗逆性。
Nanoceria-induced variations in leaf anatomy and cell wall composition drive the increase in mesophyll conductance of salt-stressed cotton leaves
Nanomaterials as an emerging tool are being used to improve plant's net photosynthetic rate (AN) when suffering salt stress, but the underlying mechanisms remain unclear. To clarify this, a hydroponic experiment was conducted to study the effects of polyacrylic acid coated nanoceria (PNC) on the AN of salt-stressed cotton and related intrinsic mechanisms. Results showed that the PNC-induced AN enhancement of salt-stressed leaves was strongly facilitated by the mesophyll conductance to CO2 (gm). Further analysis showed that the PNC-induced improvement of gm was related to the increased chloroplast surface area exposed to intercellular airspaces, which was attribute to the increased mesophyll surface area exposed to intercellular airspaces and chloroplast number due to the increased K+ content and decreased reactive oxygen species level in salt-stressed leaves. Interestingly, our results also showed that PNC-induced variations in cell wall composition of salt-stressed cotton leaves strongly influenced gm, especially, hemicellulose and pectin. Moreover, the proportion of pectin in cell wall composition played a more important role in determining gm. Our study demonstrated for the first time that nanoceria, through alterations to anatomical traits and cell wall composition, drove gm enhancement, which ultimately increased AN of salt-stressed leaves.
期刊介绍:
Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement.
Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB.
Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.