An enhanced chlorophyll estimation model with a canopy structural trait in maize crops: Use of multi-spectral UAV images and machine learning algorithm

Leaf chlorophyll concentration (LCC) is a key indicator of leaf nitrogen (N) and changes in canopy structure, particularly the leaf area index (LAI), play a significant role in estimating LCC. Spectral prediction models for chlorophyll are a useful tool for timely nutritional management, particularly in precision agriculture. However, the accuracy of LCC estimation is influenced by the LAI. Considering LAI data as input in the spectral prediction model is still inadequate for improving LCC estimation. This study tested the hypothesis that LCC estimate accuracy could be enhanced by using LAI as an input using high-resolution (5 cm) multi-spectral images from an unmanned aerial vehicle (UAV). For this, maize was grown as a test crop under different nutrient management in the hilly ecosystem of Meghalaya. LCC was measured using laboratory destruction methods from ground sampling that coincided with UAV flights. Machine learning algorithms such as random forest (RF), support vector machine (SVM), and kernel ridge regression (KKR) were employed to develop the LCC estimation model, utilizing band reflectance, vegetation indexes, and measured chlorophyll. The model was assessed for its sensitivity to LCC estimation using LAI data. KKR outperformed other two algorithms (RF and SVM) in accuracy of LCC estimation by >11.0 to 19.0 %. The KKR-derived LCC estimation model was significantly improved by the inclusion of LAI (R² increased from 0.785 to 0.928 and RMSE decreased from 0.065 to 0.053 mg g⁻¹). The model's reliability was proven on multiple UAV flights for maize crops that are healthy and nutrient-stressed. Thus, LCC models derived from multispectral UAV images using KKR algorithms could benefit the adoption of precision agriculture at field scale in mountain ecosystems.