pSPObooster：提高酿酒酵母实验室菌株繁殖效率的质粒系统

IF 2.2 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Yeast Pub Date : 2024-09-09 DOI:10.1002/yea.3978

Raphael Loll-Krippleber, Yangyang Kate Jiang, Grant W Brown

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引用次数: 0

摘要

源自 S288C 的普通酿酒酵母实验菌株存在减数分裂缺陷，因此孢子发育不良。在这里，我们开发了一种含有 MKT1 和 RME1 基因校正等位基因的质粒系统来挽救减数分裂缺陷，并证明含有该质粒的标准 BY4741 和 BY4742 株系能更快更有效地产生孢子。这种名为 pSPObooster 的质粒可以作为外显子保持，并且很容易固化或稳定地整合到基因组的单个位点上。我们展示了 pSPObooster 在低通量和高通量酵母遗传操作中的应用，并证明它能加快这两种操作过程而不影响菌株的行为。

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pSPObooster: A Plasmid System to Improve Sporulation Efficiency of Saccharomyces cerevisiae Lab Strains.

Common Saccharomyces cerevisiae lab yeast strains derived from S288C have meiotic defects and therefore are poor sporulators. Here, we developed a plasmid system containing corrected alleles of the MKT1 and RME1 genes to rescue the meiotic defects and show that standard BY4741 and BY4742 strains containing the plasmid display faster and more efficient sporulation. The plasmid, pSPObooster, can be maintained as an episome and easily cured or stably integrated into the genome at a single locus. We demonstrate the use of pSPObooster in low- and high-throughput yeast genetic manipulations and show that it can expedite both procedures without impacting strain behavior.

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来源期刊

Yeast 生物-生化与分子生物学

CiteScore

5.30

自引率

3.80%

发文量

审稿时长

3 months

期刊介绍： Yeast publishes original articles and reviews on the most significant developments of research with unicellular fungi, including innovative methods of broad applicability. It is essential reading for those wishing to keep up to date with this rapidly moving field of yeast biology. Topics covered include: biochemistry and molecular biology; biodiversity and taxonomy; biotechnology; cell and developmental biology; ecology and evolution; genetics and genomics; metabolism and physiology; pathobiology; synthetic and systems biology; tools and resources