Gγ蛋白 GS3 与 OsmiR396/GS2 在调控水稻籽粒大小方面的紧密遗传关系

IF 4.8 1区 农林科学 Q1 AGRONOMY
Rice Pub Date : 2024-09-09 DOI:10.1186/s12284-024-00736-6
Lin Zhu, Yanjie Shen, Zhengyan Dai, Xuexia Miao, Zhenying Shi
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引用次数: 0

摘要

由于谷粒大小与谷粒重量密切相关,因此控制谷粒大小在提高谷物产量方面具有巨大潜力。在水稻中已经阐述了几种调节粒度的途径,但很少研究这些成分之间可能存在的相互影响。OsmiR396 通过靶向 OsGRF4(GS2)和 OsGRF8 负向调节谷粒大小,并被证明具有多种功能。在此,我们发现在MIM396、GRF8OE和GS2OE植株的幼胚中,负向调控粒径的Gγ蛋白编码基因GS3基因的表达大幅下调,表明OsmiR396/GRF模块可能调控了GS3基因。同时,多种生化实验证明 OsGRF4 和 OsGRF8 蛋白可能对 GS3 基因有转录调控作用。进一步的遗传关系分析表明,不仅 OsmiR396 和 GS3 基因之间存在紧密的遗传关联,而且 GS2 和 GS3 基因之间也存在紧密的遗传关联。此外,我们还发现了 GS2 对其他四个谷粒大小调控 G 蛋白编码基因的可能调控。因此,OsmiR396通路和G蛋白通路在调控谷粒大小方面存在交叉作用。因此,我们建立了一个连接 miRNA-转录因子通路和 G 蛋白信号通路的桥梁,从而调控水稻的粒度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Gγ-protein GS3 Function in Tight Genetic Relation with OsmiR396/GS2 to Regulate Grain Size in Rice.

Gγ-protein GS3 Function in Tight Genetic Relation with OsmiR396/GS2 to Regulate Grain Size in Rice.

Manipulating grain size demonstrates great potential for yield promotion in cereals since it is tightly associated with grain weight. Several pathways modulating grain size have been elaborated in rice, but possible crosstalk between the ingredients is rarely studied. OsmiR396 negatively regulates grain size through targeting OsGRF4 (GS2) and OsGRF8, and proves to be multi-functioning. Here we showed that expression of GS3 gene, a Gγ-protein encoding gene, that negatively regulates grain size, was greatly down-regulated in the young embryos of MIM396, GRF8OE and GS2OE plants, indicating possible regulation of GS3 gene by OsmiR396/GRF module. Meanwhile, multiple biochemical assays proved possible transcriptional regulation of OsGRF4 and OsGRF8 proteins on GS3 gene. Further genetic relation analysis revealed tight genetic association between not only OsmiR396 and GS3 gene, but also GS2 and GS3 gene. Moreover, we revealed possible regulation of GS2 on four other grain size-regulating G protein encoding genes. Thus, the OsmiR396 pathway and the G protein pathway cross talks to regulate grain size. Therefore, we established a bridge linking the miRNA-transcription factors pathway and the G-protein signaling pathway that regulates grain size in rice.

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来源期刊
Rice
Rice AGRONOMY-
CiteScore
10.10
自引率
3.60%
发文量
60
审稿时长
>12 weeks
期刊介绍: Rice aims to fill a glaring void in basic and applied plant science journal publishing. This journal is the world''s only high-quality serial publication for reporting current advances in rice genetics, structural and functional genomics, comparative genomics, molecular biology and physiology, molecular breeding and comparative biology. Rice welcomes review articles and original papers in all of the aforementioned areas and serves as the primary source of newly published information for researchers and students in rice and related research.
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