{"title":"Serratia marcescens 中 blaKPC-2 和 blaNDM-1 的宿主内和种间转移及其地方和全球流行病学。","authors":"","doi":"10.1016/j.ijantimicag.2024.107327","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><div>The aim of this study was to investigate interspecies transfer of resistance gene <em>bla</em><sub>NDM-1</sub> and intraspecies transfer of resistance gene <em>bla</em><sub>KPC-2</sub> in <em>Serratia marcescens</em>, and explore the epidemical and evolutionary characteristics of carbapenemase-producing <em>S. marcescens</em> (CPSM) regionally and globally.</div></div><div><h3>Methods</h3><div>Interspecies and intraspecies transfer of <em>bla</em><sub>KPC-2</sub>- or <em>bla</em><sub>NDM-1</sub> were identified by antimicrobial susceptibility testing, plasmid conjugation and curing, discovery of transposable units (TUs), outer membrane vesicles (OMVs), qPCR, whole-genome sequencing (WGS) and bioinformatic analysis. The genomic evolution of CPSM strains was explored by cgSNP and maximum-likelihood phylogenetic tree.</div></div><div><h3>Results</h3><div>CPSM S50079 strain, co-carrying <em>bla</em><sub>KPC-2</sub> and <em>bla</em><sub>NDM-1</sub> on one plasmid, was isolated from the blood of a patient with acute pancreatitis and could generate TUs carrying either <em>bla</em><sub>KPC-2</sub> or <em>bla</em><sub>NDM-1</sub>. The interspecies transfer of <em>bla</em><sub>NDM-1</sub>-carrying plasmid from <em>Providencia rettgeri</em> P50213, producing the identical <em>bla</em><sub>NDM-1</sub>-carrying TUs, to <em>S. marcescens</em> S50079K, an S50079 variant <em>via</em> plasmid curing, was identified through <em>bla</em><sub>NDM-1</sub>-harbouring plasmid conjugation and OMVs transfer. Moreover, the intraspecies transfer of <em>bla</em><sub>KPC-2</sub>, mediated by IS<em>26</em> from plasmid to chromosome in S50079, was also identified. In another patient, who underwent lung transplantation, interspecies transfer of <em>bla</em><sub>NDM-1</sub> carried by IncX3 plasmid was identified among <em>S. marcescens</em> and <em>Citrobacter freundii</em> as well as <em>Enterobacter hormaechei via</em> plasmid transfer. Furthermore, 11 CPSM from 349 non-repetitive <em>S. marcescens</em> strains were identified in the same hospital, and clonal dissemination, with carbapenemase evolution from <em>bla</em><sub>KPC-2</sub> to both <em>bla</em><sub>KPC-2</sub> and <em>bla</em><sub>NDM-1</sub>, was found in the 8 CPSM across 4 years. Finally, the analysis of 236 global CPSM from 835 non-repetitive <em>S. marcescens</em> genomes, retrieved from the NCBI database, revealed long-term spread and evolution worldwide, and would cause the convergence of more carbapenemase genes.</div></div><div><h3>Conclusions</h3><div>Interspecies transfer of resistance gene <em>bla</em><sub>NDM-1</sub> and intraspecies transfer of resistance gene <em>bla</em><sub>KPC-2</sub> in CPSM were identified. Nosocomial and global dissemination of CPSM were revealed and more urgent surveillance was acquired.</div></div>","PeriodicalId":13818,"journal":{"name":"International Journal of Antimicrobial Agents","volume":null,"pages":null},"PeriodicalIF":4.9000,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In-host intra- and inter-species transfer of blaKPC-2 and blaNDM-1 in Serratia marcescens and its local and global epidemiology\",\"authors\":\"\",\"doi\":\"10.1016/j.ijantimicag.2024.107327\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><div>The aim of this study was to investigate interspecies transfer of resistance gene <em>bla</em><sub>NDM-1</sub> and intraspecies transfer of resistance gene <em>bla</em><sub>KPC-2</sub> in <em>Serratia marcescens</em>, and explore the epidemical and evolutionary characteristics of carbapenemase-producing <em>S. marcescens</em> (CPSM) regionally and globally.</div></div><div><h3>Methods</h3><div>Interspecies and intraspecies transfer of <em>bla</em><sub>KPC-2</sub>- or <em>bla</em><sub>NDM-1</sub> were identified by antimicrobial susceptibility testing, plasmid conjugation and curing, discovery of transposable units (TUs), outer membrane vesicles (OMVs), qPCR, whole-genome sequencing (WGS) and bioinformatic analysis. The genomic evolution of CPSM strains was explored by cgSNP and maximum-likelihood phylogenetic tree.</div></div><div><h3>Results</h3><div>CPSM S50079 strain, co-carrying <em>bla</em><sub>KPC-2</sub> and <em>bla</em><sub>NDM-1</sub> on one plasmid, was isolated from the blood of a patient with acute pancreatitis and could generate TUs carrying either <em>bla</em><sub>KPC-2</sub> or <em>bla</em><sub>NDM-1</sub>. The interspecies transfer of <em>bla</em><sub>NDM-1</sub>-carrying plasmid from <em>Providencia rettgeri</em> P50213, producing the identical <em>bla</em><sub>NDM-1</sub>-carrying TUs, to <em>S. marcescens</em> S50079K, an S50079 variant <em>via</em> plasmid curing, was identified through <em>bla</em><sub>NDM-1</sub>-harbouring plasmid conjugation and OMVs transfer. Moreover, the intraspecies transfer of <em>bla</em><sub>KPC-2</sub>, mediated by IS<em>26</em> from plasmid to chromosome in S50079, was also identified. In another patient, who underwent lung transplantation, interspecies transfer of <em>bla</em><sub>NDM-1</sub> carried by IncX3 plasmid was identified among <em>S. marcescens</em> and <em>Citrobacter freundii</em> as well as <em>Enterobacter hormaechei via</em> plasmid transfer. Furthermore, 11 CPSM from 349 non-repetitive <em>S. marcescens</em> strains were identified in the same hospital, and clonal dissemination, with carbapenemase evolution from <em>bla</em><sub>KPC-2</sub> to both <em>bla</em><sub>KPC-2</sub> and <em>bla</em><sub>NDM-1</sub>, was found in the 8 CPSM across 4 years. Finally, the analysis of 236 global CPSM from 835 non-repetitive <em>S. marcescens</em> genomes, retrieved from the NCBI database, revealed long-term spread and evolution worldwide, and would cause the convergence of more carbapenemase genes.</div></div><div><h3>Conclusions</h3><div>Interspecies transfer of resistance gene <em>bla</em><sub>NDM-1</sub> and intraspecies transfer of resistance gene <em>bla</em><sub>KPC-2</sub> in CPSM were identified. Nosocomial and global dissemination of CPSM were revealed and more urgent surveillance was acquired.</div></div>\",\"PeriodicalId\":13818,\"journal\":{\"name\":\"International Journal of Antimicrobial Agents\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.9000,\"publicationDate\":\"2024-09-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Antimicrobial Agents\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0924857924002437\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Antimicrobial Agents","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0924857924002437","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
In-host intra- and inter-species transfer of blaKPC-2 and blaNDM-1 in Serratia marcescens and its local and global epidemiology
Objectives
The aim of this study was to investigate interspecies transfer of resistance gene blaNDM-1 and intraspecies transfer of resistance gene blaKPC-2 in Serratia marcescens, and explore the epidemical and evolutionary characteristics of carbapenemase-producing S. marcescens (CPSM) regionally and globally.
Methods
Interspecies and intraspecies transfer of blaKPC-2- or blaNDM-1 were identified by antimicrobial susceptibility testing, plasmid conjugation and curing, discovery of transposable units (TUs), outer membrane vesicles (OMVs), qPCR, whole-genome sequencing (WGS) and bioinformatic analysis. The genomic evolution of CPSM strains was explored by cgSNP and maximum-likelihood phylogenetic tree.
Results
CPSM S50079 strain, co-carrying blaKPC-2 and blaNDM-1 on one plasmid, was isolated from the blood of a patient with acute pancreatitis and could generate TUs carrying either blaKPC-2 or blaNDM-1. The interspecies transfer of blaNDM-1-carrying plasmid from Providencia rettgeri P50213, producing the identical blaNDM-1-carrying TUs, to S. marcescens S50079K, an S50079 variant via plasmid curing, was identified through blaNDM-1-harbouring plasmid conjugation and OMVs transfer. Moreover, the intraspecies transfer of blaKPC-2, mediated by IS26 from plasmid to chromosome in S50079, was also identified. In another patient, who underwent lung transplantation, interspecies transfer of blaNDM-1 carried by IncX3 plasmid was identified among S. marcescens and Citrobacter freundii as well as Enterobacter hormaechei via plasmid transfer. Furthermore, 11 CPSM from 349 non-repetitive S. marcescens strains were identified in the same hospital, and clonal dissemination, with carbapenemase evolution from blaKPC-2 to both blaKPC-2 and blaNDM-1, was found in the 8 CPSM across 4 years. Finally, the analysis of 236 global CPSM from 835 non-repetitive S. marcescens genomes, retrieved from the NCBI database, revealed long-term spread and evolution worldwide, and would cause the convergence of more carbapenemase genes.
Conclusions
Interspecies transfer of resistance gene blaNDM-1 and intraspecies transfer of resistance gene blaKPC-2 in CPSM were identified. Nosocomial and global dissemination of CPSM were revealed and more urgent surveillance was acquired.
期刊介绍:
The International Journal of Antimicrobial Agents is a peer-reviewed publication offering comprehensive and current reference information on the physical, pharmacological, in vitro, and clinical properties of individual antimicrobial agents, covering antiviral, antiparasitic, antibacterial, and antifungal agents. The journal not only communicates new trends and developments through authoritative review articles but also addresses the critical issue of antimicrobial resistance, both in hospital and community settings. Published content includes solicited reviews by leading experts and high-quality original research papers in the specified fields.