PCR 测序和新一代测序在孢子丝菌病诊断中的应用。

IF 1.9 4区 医学 Q3 DERMATOLOGY
Clinical, Cosmetic and Investigational Dermatology Pub Date : 2024-09-03 eCollection Date: 2024-01-01 DOI:10.2147/CCID.S473442
Xinyu Yang, Xiumin Yang
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引用次数: 0

摘要

孢子丝菌病是一种常见的慢性真菌感染,临床表现往往不典型。孢子丝菌病的诊断主要依靠真菌培养、组织病理学检查和分子检测鉴定。我们曾报告过一名 70 岁的男子,他的右手背出现皮肤病变(已出现 6 个月)。他在当地医院确诊为皮肤细菌感染,但病变一直未见好转。体格检查显示,他的手背上有浸润性淡红色斑块,伴有脓性分泌物和结痂。组织病理学检查发现真皮层中有散在的圆形酵母细胞。真菌培养发现,沙保葡萄糖琼脂(SDA)上有多个棕色绒毛状菌落。通过 PCR 测序和新一代测序(NGS)方法,确定了球孢子菌。最后,通过组织病理学检查、真菌学检查和分子鉴定,确诊了一例由球孢子虫引起的孢子丝虫病。患者接受了伊曲康唑 400 毫克/天的口服治疗,为期 2 个月。病变明显好转。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Application of PCR Sequencing and Next-Generation Sequencing in the Diagnosis of Sporotrichosis.

Sporotrichosis is a common chronic fungal infection and the clinical manifestations are often untypical. Diagnosis of sporotrichosis relies conventionally on fungal culture, histopathological examination, and species identification by molecular test. We reported that a 70-year-old man presented with a cutaneous lesion on the back of his right hand (present for 6 months). The cutaneous bacterial infection was diagnosed at a local hospital and the lesion had not improved. Physical examination revealed an infiltrative reddish plaque with purulent secretion and crusts. Histopathological examination revealed scattered round yeast cells in the dermis. Fungal culture revealed multiple, velvety, brown colonies on Sabouraud dextrose agar (SDA). Sporothrix globosa was identified by PCR-sequencing and next generation sequencing (NGS) method. Finally, a case of sporotrichosis caused by Sporothrix globosa was diagnosed by histopathological examination, mycological examination, and molecular identification. The patient was treated with oral itraconazole 400 mg/day for 2 months. The lesion was dramatically ameliorated.

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来源期刊
CiteScore
2.80
自引率
4.30%
发文量
353
审稿时长
16 weeks
期刊介绍: Clinical, Cosmetic and Investigational Dermatology is an international, peer-reviewed, open access journal that focuses on the latest clinical and experimental research in all aspects of skin disease and cosmetic interventions. Normal and pathological processes in skin development and aging, their modification and treatment, as well as basic research into histology of dermal and dermal structures that provide clinical insights and potential treatment options are key topics for the journal. Patient satisfaction, preference, quality of life, compliance, persistence and their role in developing new management options to optimize outcomes for target conditions constitute major areas of interest. The journal is characterized by the rapid reporting of clinical studies, reviews and original research in skin research and skin care. All areas of dermatology will be covered; contributions will be welcomed from all clinicians and basic science researchers globally.
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