DWARF 和 SELF-PRUNING 的基因组编辑可快速赋予番茄适合植物工厂的性状,同时保留番茄的有用性状。

IF 2 4区 农林科学 Q2 AGRONOMY
Breeding Science Pub Date : 2024-03-01 Epub Date: 2024-04-04 DOI:10.1270/jsbbs.23063
Ai Nagamine, Hiroshi Ezura
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引用次数: 0

摘要

在作物栽培过程中,人工光照植物工厂受环境因素的影响比露天地区小,作为解决世界粮食问题的方法之一备受关注。然而,植物工厂的种植成本高于露天种植,而且目前工厂种植的农作物品种仅限于小型或生长期较短的品种,利润较低。西红柿是全球消费的主要农作物之一,但由于其植株高度和宽度较大,尚不适合在植物工厂进行大规模生产。本研究通过 CRISPR-Cas9 方法对 GABA 高积累番茄品种 #87-17 的 DWARF(D)和 SELF-PRUNING (SP)基因进行基因组编辑,以培育矮小番茄植株。T1 基因组编辑世代获得了所需的性状,果实性状与原品种基本相同。另一方面,#87-17 与含有 d 和 sp 突变的 Micro-Tom 杂交的 F2 植株出现矮化,但果实表型混合了两个品种的性状。这表明,利用 CRISPR-Cas9 对这两个基因进行基因组编辑,可以有效地赋予适合工厂化栽培的性状,同时保留原栽培品种的有用性状。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genome editing of DWARF and SELF-PRUNING rapidly confers traits suitable for plant factories while retaining useful traits in tomato.

Plant factories with artificial light are less affected than open-air areas to environmental factors in crop cultivation and are attracting attention as one of the solutions to the world's food problems. However, the cost of cultivation in plant factories is higher than open-air cultivation, and currently, profitable factory-grown crop varieties are limited to those that are small or have a short growing period. Tomatoes are one of the main crops consumed around the world, but due to their large plant height and width, they are not yet suitable for mass production in plant factories. In this study, the DWARF (D) and SELF-PRUNING (SP) genes of the GABA hyperaccumulating tomato variety #87-17 were genome-edited by the CRISPR-Cas9 method to produce dwarf tomato plants. The desired traits were obtained in the T1 genome-edited generation, and the fruit traits were almost the same as those of the original variety. On the other hand, the F2 cross between #87-17 and Micro-Tom containing the d and sp mutations was dwarfed, but the fruit phenotype was a mixture of the traits of the two varieties. This indicates that genome editing of these two genes using CRISPR-Cas9 can efficiently impart traits suitable for plant factory cultivation while retaining the useful traits of the original cultivar.

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来源期刊
Breeding Science
Breeding Science 农林科学-农艺学
CiteScore
4.90
自引率
4.20%
发文量
37
审稿时长
1.5 months
期刊介绍: Breeding Science is published by the Japanese Society of Breeding. Breeding Science publishes research papers, notes and reviews related to breeding. Research Papers are standard original articles. Notes report new cultivars, breeding lines, germplasms, genetic stocks, mapping populations, database, software, and techniques significant and useful for breeding. Reviews summarize recent and historical events related breeding. Manuscripts should be submitted by corresponding author. Corresponding author must have obtained permission from all authors prior to submission. Correspondence, proofs, and charges of excess page and color figures should be handled by the corresponding author.
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