由茄科镰刀菌中β-葡萄糖苷酶产生的潜在益生元纤维寡糖的生物合成和一步法富集过程。

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Manel Boudabbous, Ines Ben Hmad, Mariem Zaidi, Walid Saibi, Lobna Jlaiel, Ali Gargouri
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引用次数: 0

摘要

胞寡糖(COS)是一种新型的功能性寡糖。为了提高 COS 的产量,对 COS 的转糖基化反应进行了研究。在寻找游离型溶菌镰刀菌β-葡萄糖苷酶(FBgl1)在低底物浓度下合成 COS 的能力时,我们发现这种生物催化剂只需 1 克/升的纤维生物糖就能启动该反应,形成纤维三糖。在固定 FBgl1 的双相条件下,以及将起始浓度提高到 50 克/升纤维生物糖时,检测到了纤维三糖和纤维戊糖。经过生物催化剂循环处理后,COS 的反式糖基化产率在 5 个循环后保持不变,COS 浓度为 6.70 ± 0.35 g/L。粗 COS 含 20.15 ± 0.25 g/L 葡萄糖、23.15 ± 0.22 g/L 非反应底物纤维生物糖、5.25 ± 0.53 g/L 细胞三糖和 1.49 ± 0.32 g/L 细胞戊糖。利用完整的 Velezensis 杆菌细胞作为微生物纯化工具,开发了一种富集纤维三糖的生物工艺。这种细菌消耗葡萄糖、未反应的纤维生物糖和纤维戊糖,同时保留发酵培养基中的纤维三糖。这项研究为工业化生产 COS 提供了一种极佳的候选酶,同时也是对单步 COS 富集过程的首次研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Biosynthesis and one-step enrichment process of potentially prebiotic cello-oligosaccharides produced by β-glucosidase from Fusarium solani

Biosynthesis and one-step enrichment process of potentially prebiotic cello-oligosaccharides produced by β-glucosidase from Fusarium solani

Cello-oligosaccharides (COS) become a new type of functional oligosaccharides. COS transglycosylation reactions were studied to enhance COS yield production. Seeking the ability of the free form of Fusarium solani β-glucosidase (FBgl1) to synthesize COS under low substrate concentrations, we found out that this biocatalyst initiates this reaction with only 1 g/L of cellobiose, giving rise to the formation of cellotriose. Cellotriose and cellopentaose were detected in biphasic conditions with an immobilized FBgl1 and when increased to 50 g/L of cellobiose as a starter concentration. After the biocatalyst recycling process, the trans-glycosylation yield of COS was maintained after 5 cycles, and the COS concentration was 6.70 ± 0.35 g/L. The crude COS contained 20.15 ± 0.25 g/L glucose, 23.15 ± 0.22 g/L non-reacting substrate cellobiose, 5.25 ± 0.53 g/L, cellotriose and 1.49 ± 0.32 g/L cellopentaose. A bioprocess was developed for cellotriose enrichment, using whole Bacillus velezensis cells as a microbial purification tool. This bacteria consumed glucose, unreacted cellobiose, and cellopentaose while preserving cellotriose in the fermented medium. This study provides an excellent enzyme candidate for industrial COS production and is also the first study on the single-step COS enrichment process.

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来源期刊
Archives of Microbiology
Archives of Microbiology 生物-微生物学
CiteScore
4.90
自引率
3.60%
发文量
601
审稿时长
3 months
期刊介绍: Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.
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