马来西亚半岛牛蜱（Acari: Ixodidae）中与蜱相关的锥虫（Apicomplexa: Kinetoplastida）。

IF 2.1 3区医学 Q2 PARASITOLOGY

Acta tropica Pub Date : 2024-09-06 DOI:10.1016/j.actatropica.2024.107383

Abdul Rahman Kazim , Van Lun Low , Jamal Houssaini , Dennis Tappe , Chong Chin Heo

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引用次数: 0

摘要

对从马来西亚半岛 674 个选定的农场反刍动物身上采集的 130 个蜱池中的 1,241 只蜱进行了锥虫筛查。其中，9 个样本库的锥虫检测结果呈阳性。随后的 BLAST 搜索显示，18S rRNA 基因序列与 Chaco CB 分离物中的红臀锥虫密切相关，相似度为 95.56% 至 99.84%。系统进化分析表明，9 个序列中有 3 个与赤脊锥虫形成一个支系。其余 6 个锥虫序列形成了一个独立的支系，与红臀锥虫和其他锥虫物种分开，遗传距离分别为 4.34% 和 4.33-4.58%。这项研究标志着马来西亚首次报告与蜱相关的锥虫，并凸显了该地区锥虫与蜱宿主相互作用方面的重大研究空白。

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Tick-associated Trypanosoma species (Apicomplexa: Kinetoplastida) from cattle ticks (Acari: Ixodidae) in Peninsular Malaysia

A Trypanosoma screening was conducted on 130 pools comprising 1,241 ticks, collected from 674 selected farm ruminants in Peninsular Malaysia. Of these, nine pools were tested positive for Trypanosoma. Subsequent BLAST searches revealed that the 18S rRNA gene sequences were closely related to Trypanosoma rhipicephalis isolate Chaco CB, with percentage similarities ranging from 95.56 % to 99.84 %. Phylogenetic analysis showed that three of the nine sequences formed a clade with Trypanosoma rhipicephalis. The remaining six Trypanosoma sequences formed a distinct clade, separate from T. rhipicephalis and other Trypanosoma species, with genetic distances of 4.34 % and 4.33–4.58 %, respectively. This study marks the first report of tick-associated Trypanosoma in Malaysia and underscores significant research gaps regarding trypanosome interactions with tick hosts in the region.

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来源期刊

Acta tropica 医学-寄生虫学

CiteScore

5.40

自引率

11.10%

发文量

383

审稿时长

37 days

期刊介绍： Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.