长期服用羟考酮对成年雄性大鼠小脑和纹状体行为功能和组织病理学的影响

IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
3 Biotech Pub Date : 2024-10-01 Epub Date: 2024-09-06 DOI:10.1007/s13205-024-04062-y
Farzin Banei, Abbas Aliaghaei, Gholam Hossein Meftahi
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引用次数: 0

摘要

羟考酮被广泛用于止痛,通过与μ-和卡巴阿片受体结合发挥作用。研究表明,长期使用羟考酮可通过应激反应导致神经元脱髓鞘和变性,从而引发信号通路凋亡。纹状体和小脑被认为是导致成瘾的重要因素,但目前还没有关于羟考酮对小脑和纹状体以及运动协调性影响的报告。我们每天以 15 毫克/千克的剂量对大鼠进行为期 30 天的羟考酮治疗。然后对大鼠的运动表现和肌电图活动进行评估。我们用立体学方法评估了小脑和纹状体中神经元的数量,并用免疫组化方法检测了小神经胶质细胞和星形胶质细胞。此外,还利用 Sholl 分析法评估了小胶质细胞和星形胶质细胞的细胞结构。运动协调性的转体测试结果显示,小胶质细胞和星形胶质细胞没有明显(P P P P P
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The effect of chronic administration of oxycodone on the behavioral functions and histopathology in the cerebellum and striatum of adult male rats.

The effect of chronic administration of oxycodone on the behavioral functions and histopathology in the cerebellum and striatum of adult male rats.

Oxycodone is widely used for pain management and acts via binding to mu- and kappa opioid receptors. It was shown that extended oxycodone usage can result from the demyelination and degeneration of neurons through the stress response, which triggers apoptotic signaling pathways. The striatum and cerebellum are recognized as significant contributors to addiction; however, there is no report on the effect of oxycodone on the cerebellum and striatum and motor coordination. We treated rats daily with oxycodone at 15 mg/kg doses for thirty days. Motor performance and electromyography activity were then evaluated. Stereological methods were performed to assess the number of neurons in the cerebellum and striatum as well as immunohistochemistry for microgliosis and astrogliosis. Furthermore, the Sholl analysis method was utilized to evaluate the cellular structure of both microglia and astrocytes. Results of the rotarod test for motor coordination show no significant (P < 0.05) difference between the oxycodone subjects and those in the control group. In addition, open-field assessments indicated that the application of oxycodone did not alter the amount of distance covered (as an indicator of locomotion) or time spent in the central area (as an indicator of anxiety) (P < 0.001). The electromyography (EMG) test result showed that oxycodone caused a delay in the reaction of the muscular nerves (P < 0.001). Data and results from our experiment revealed that administering oxycodone did not affect astrogliosis and the number of neurons in the cerebellum and striatum (P < 0.05). In contrast, it altered neuromuscular function. In addition, oxycodone administration activated microglia in the cerebellum and striatum. In conclusion, we encourage more research on the adverse effects of oxycodone on the brain.

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来源期刊
3 Biotech
3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
6.00
自引率
0.00%
发文量
314
期刊介绍: 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.
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