{"title":"基于信号放大策略的近红外光激活 DNA 纳米机械用于活细胞中 microRNA-21 的时空可控监测","authors":"","doi":"10.1016/j.bios.2024.116755","DOIUrl":null,"url":null,"abstract":"<div><p>Precise and spatiotemporally controllable analysis of microRNA-21 in living cells is crucial for accurate diagnosis and effective treatment of related diseases. Herein, a near-infrared (NIR)-photoactivatable DNA nanomachine (PUCNPs-NH<sub>2</sub>/PEG-ZL-DNA) was constructed for the precise analysis and diagnosis of microRNA-21 in tumor cells. Peanut-shaped upconversion nanoparticles (PUCNPs) were employed as the carriers and activators for the intelligent DNA probe, specifically enabling the cleavage of the photocleavable linker (PC-linker) from the hairpin DNA probe (Hp-Dzy) upon exposure to 808 nm irradiation. In the presence of the target microRNA-21, the locker DNA hybridized with microRNA-21 and the DNAzymes was freed to hybridize with the looped portion of the hairpin DNA (Hp-1). Mg<sup>2+</sup> was employed as the cofactor, facilitating the precise cleavage of Hp-1, which triggered the restoration of fluorescence signals. Subsequently, DNAzymes exhibited the competency to selectively recognize and engage with additional Hp-1, and the fluorescence signals were effectively amplified by the recycling process. Consequently, the DNA nanomachine exhibited a linear response to microRNA-21 concentrations ranging from 0.5 nM to 1.0 μM, achieving a remarkable detection limit (LOD) of 1.19 nM under the optimal conditions. This strategy is realized through the integration of photocontrollable upconversion nanotechnology with the signal amplification approach, showing feasible prospects for spatiotemporally precise and highly sensitive monitoring of microRNA in tumor cells.</p></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7000,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"NIR-photoactivatable DNA nanomachines for spatiotemporally controllable monitoring of microRNA-21 in living cells based on signal amplification strategy\",\"authors\":\"\",\"doi\":\"10.1016/j.bios.2024.116755\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Precise and spatiotemporally controllable analysis of microRNA-21 in living cells is crucial for accurate diagnosis and effective treatment of related diseases. Herein, a near-infrared (NIR)-photoactivatable DNA nanomachine (PUCNPs-NH<sub>2</sub>/PEG-ZL-DNA) was constructed for the precise analysis and diagnosis of microRNA-21 in tumor cells. Peanut-shaped upconversion nanoparticles (PUCNPs) were employed as the carriers and activators for the intelligent DNA probe, specifically enabling the cleavage of the photocleavable linker (PC-linker) from the hairpin DNA probe (Hp-Dzy) upon exposure to 808 nm irradiation. In the presence of the target microRNA-21, the locker DNA hybridized with microRNA-21 and the DNAzymes was freed to hybridize with the looped portion of the hairpin DNA (Hp-1). Mg<sup>2+</sup> was employed as the cofactor, facilitating the precise cleavage of Hp-1, which triggered the restoration of fluorescence signals. Subsequently, DNAzymes exhibited the competency to selectively recognize and engage with additional Hp-1, and the fluorescence signals were effectively amplified by the recycling process. Consequently, the DNA nanomachine exhibited a linear response to microRNA-21 concentrations ranging from 0.5 nM to 1.0 μM, achieving a remarkable detection limit (LOD) of 1.19 nM under the optimal conditions. This strategy is realized through the integration of photocontrollable upconversion nanotechnology with the signal amplification approach, showing feasible prospects for spatiotemporally precise and highly sensitive monitoring of microRNA in tumor cells.</p></div>\",\"PeriodicalId\":259,\"journal\":{\"name\":\"Biosensors and Bioelectronics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":10.7000,\"publicationDate\":\"2024-09-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biosensors and Bioelectronics\",\"FirstCategoryId\":\"1\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0956566324007619\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOPHYSICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biosensors and Bioelectronics","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0956566324007619","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOPHYSICS","Score":null,"Total":0}
NIR-photoactivatable DNA nanomachines for spatiotemporally controllable monitoring of microRNA-21 in living cells based on signal amplification strategy
Precise and spatiotemporally controllable analysis of microRNA-21 in living cells is crucial for accurate diagnosis and effective treatment of related diseases. Herein, a near-infrared (NIR)-photoactivatable DNA nanomachine (PUCNPs-NH2/PEG-ZL-DNA) was constructed for the precise analysis and diagnosis of microRNA-21 in tumor cells. Peanut-shaped upconversion nanoparticles (PUCNPs) were employed as the carriers and activators for the intelligent DNA probe, specifically enabling the cleavage of the photocleavable linker (PC-linker) from the hairpin DNA probe (Hp-Dzy) upon exposure to 808 nm irradiation. In the presence of the target microRNA-21, the locker DNA hybridized with microRNA-21 and the DNAzymes was freed to hybridize with the looped portion of the hairpin DNA (Hp-1). Mg2+ was employed as the cofactor, facilitating the precise cleavage of Hp-1, which triggered the restoration of fluorescence signals. Subsequently, DNAzymes exhibited the competency to selectively recognize and engage with additional Hp-1, and the fluorescence signals were effectively amplified by the recycling process. Consequently, the DNA nanomachine exhibited a linear response to microRNA-21 concentrations ranging from 0.5 nM to 1.0 μM, achieving a remarkable detection limit (LOD) of 1.19 nM under the optimal conditions. This strategy is realized through the integration of photocontrollable upconversion nanotechnology with the signal amplification approach, showing feasible prospects for spatiotemporally precise and highly sensitive monitoring of microRNA in tumor cells.
期刊介绍:
Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.