通过基于药敏试验的算法确定耐碳青霉烯类的铜绿假单胞菌分离物中是否存在碳青霉烯酶酶

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2024-08-14 DOI:10.1016/j.diagmicrobio.2024.116495

Murat Ocal , Ecem Buyukyanbolu , Mehmet Karakus , Oznur Koca , Seren Tanriverdi , Fatma Erdogan , Fusun Comert , Baris Otlu , Elif Aktas

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引用次数: 0

摘要

目的现已提出了检测碳青霉烯酶生产的表型方法。这些检测的周转时间较慢。方法纳入2017年1月至2021年12月期间从三家医院分离的耐碳青霉烯类铜绿假单胞菌（CRPA）。采用改良碳青霉烯酶灭活法（mCIM）和两种算法，分别定义为 "主要算法，即除亚胺培南或美罗培南耐药外，头孢他啶和头孢吡肟不耐药 "和 "次要算法，即除亚胺培南或美罗培南耐药外，头孢唑烷/他唑巴坦不耐药"。对所有分离菌株进行了 PCR 检测。当采用主要或次要算法标准时，有 173 个分离物符合其中一个或两个标准。结论在我们的研究中，使用该算法将 CIM 检测的需求降低了 32%。

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Determination of the presence of carbapenemase enzymes in carbapenem-resistant Pseudomonas aeruginosa isolates by susceptibility test based algorithm

Purpose

Phenotypic methods have been proposed for the detection of carbapenemase production. These tests can have slower turnaround times. With the sensitivity-based algorithm described by Gill et al. will be possible to detect the carbapenemase.

Methods

Carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates from three hospitals between January 2017 and December 2021 were included. The modified carbapenemase-inactivation-method(mCIM) and two algorithms were used, defined as "primary algorithm, i.e. ceftazidime and cefepime non-susceptible in addition to imipenem or meropenem resistance" and "secondary algorithm, i.e. ceftolozane/tazobactam non-susceptible in addition to imipenem or meropenem resistance". PCR testing was performed on all isolates.

Results

256 CRPA isolates were included in the study. When the primary or secondary algorithm criteria were applied, there were 173 isolates that met one or both of them. Of these, 29 were CIM-positive isolates.

Conclusion

In our study, the use of the algorithm reduced the need for CIM testing by 32 %.

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.