设计融合双链 RNA 以控制两种全球性吸汁害虫

IF 4.2 1区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
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引用次数: 0

摘要

RNA 干扰(RNAi)通过沉默目标生物体内的关键基因,是一种有效的害虫管理策略。然而,由于组织或发育阶段基因表达的特异性,针对单个基因的沉默效率往往会受到限制。此外,在当前的生态条件下,多种害虫往往会同时侵染同一种作物。因此,"靶向多个基因 "和 "控制多种害虫 "相结合的策略有望取得更好的管理效果。本研究在全基因组范围内筛选了桃蚜(Myzus persicae)和粉虱(Bemisia tabaci)这两种全球吸食汁液害虫的同源基因。随后,RNAi 生物测定显示,沉默基因(MpAbd-A、MpH3、MpRpL27a 和 MpScr)对这两种害虫的致死率都很高,因此进一步选择了这些基因设计融合 dsRNA。根据序列分析,与单基因沉默相比,这些融合 dsRNA 对两种害虫的致死率更高,而且对天敌瓢虫(Propylaea japonica)的脱靶风险极低。最后,通过病毒诱导基因沉默(VIGS)技术表达融合 dsRNAs 的烟草植株增强了对两种害虫的抗性。总之,本研究提出了一种基于 RNAi 的同时防治两种吸汁害虫的新方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Design the fusion double-strand RNAs to control two global sap-sucking pests

Design the fusion double-strand RNAs to control two global sap-sucking pests

RNA interference (RNAi) is an effective pest management strategy through silencing the crucial genes in target organisms. However, the effectiveness of targeting a single gene is often limited by the silencing efficiency due to tissue or developmental stage-specific gene expression. Moreover, multiple pests often infest the same crop simultaneously under current ecological conditions. Therefore, a combined strategy of “targeting multiple genes” and “controlling multiple pests” is expected to yield better management results. In this study, homologous genes from two globally sap-sucking pests, the peach aphid (Myzus persicae) and the whitefly (Bemisia tabaci), were screened on a genome-wide scale. Subsequently, RNAi bioassays showed silencing the genes (MpAbd-A, MpH3, MpRpL27a, and MpScr) exhibited high mortalities in both species, which were further selected for designing fusion dsRNAs. These fusion dsRNAs resulted in higher mortalities in both pests than single gene silencing and posed a minimal off-target risk to the predator ladybeetle (Propylaea japonica) based on the sequence analysis. Finally, the tobacco plants expressing the fusion dsRNAs through virus-induced gene silencing (VIGS) technology enhanced the resistance to both pests. In conclusion, this study proposes a novel RNAi-based approach for managing two sap-sucking pests simultaneously.

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来源期刊
CiteScore
7.00
自引率
8.50%
发文量
238
审稿时长
4.2 months
期刊介绍: Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.
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