Christian Bayerl , Yasmine Safraou , Rolf Reiter , Vanessa Proß , Kai Lehmann , Anja A. Kühl , Mehrgan Shahryari , Bernd Hamm , Ingolf Sack , Marcus R. Makowski , Jürgen Braun , Patrick Asbach
{"title":"通过低频和高频机械粘弹性研究肝脏炎症--磁共振弹性成像研究","authors":"Christian Bayerl , Yasmine Safraou , Rolf Reiter , Vanessa Proß , Kai Lehmann , Anja A. Kühl , Mehrgan Shahryari , Bernd Hamm , Ingolf Sack , Marcus R. Makowski , Jürgen Braun , Patrick Asbach","doi":"10.1016/j.jmbbm.2024.106711","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><p>To study the potential of viscoelastic parameters such as liver stiffness, loss tangent (marker of viscous properties) and viscoelastic dispersion to detect hepatic inflammation by <em>in-vivo</em> and <em>ex-vivo</em> MR elastography (MRE) at low and high vibration frequencies.</p></div><div><h3>Methods</h3><p>15 patients scheduled for liver tumor resection surgery were prospectively enrolled in this IRB-approved study and underwent multifrequency <em>in-vivo</em> MRE (30–60Hz) at 1.5-T prior to surgery. Immediately after liver resection, tumor-free tissue specimens were examined with <em>ex-vivo</em> MRE (0.8–2.8 kHz) at 0.5-T and histopathologic analysis including NAFLD activity score (NAS) and inflammation score (I-score) as sum of histological sub-features of inflammation.</p></div><div><h3>Results</h3><p><em>In-vivo</em><em>,</em> in regions where tissue samples were obtained, the loss tangent correlated with the I-score (R = 0.728; p = 0.002) and c-dispersion (stiffness dispersion over frequency) correlated with lobular inflammation (R = −0.559; p = 0.030). In a subgroup of patients without prior chemotherapy, c-dispersion correlated with I-score also in the whole liver (R = −0.682; p = 0.043). ROC analysis of the loss tangent for predicting the I-score showed a high AUC for I ≥ 1 (0.944; p = 0.021), I ≥ 2 (0.804; p = 0.049) and I ≥ 3 (0.944; p = 0.021). <em>Ex-vivo</em> MRE was not sensitive to inflammation, whereas strong correlations were observed between fibrosis and stiffness (R = 0.589; p = 0.021), penetration rate (R = 0.589; p = 0.021), loss tangent (R = −0.629; p = 0.012), and viscoelastic model parameters (spring-pot powerlaw exponent, R = −0.528; p = 0.043; spring-pot shear modulus, R = 0.589; p = 0.021).</p></div><div><h3>Conclusion</h3><p>Our results suggest that c-dispersion of the liver is sensitive to inflammation when measured <em>in-vivo</em> in the low dynamic range (30–60Hz), while at higher frequencies (0.8–2.8 kHz) viscoelastic parameters are dominated by fibrosis.</p></div>","PeriodicalId":380,"journal":{"name":"Journal of the Mechanical Behavior of Biomedical Materials","volume":"160 ","pages":"Article 106711"},"PeriodicalIF":3.3000,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1751616124003436/pdfft?md5=8c51f0462b02113837d352132b019949&pid=1-s2.0-S1751616124003436-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Investigation of hepatic inflammation via viscoelasticity at low and high mechanical frequencies - A magnetic resonance elastography study\",\"authors\":\"Christian Bayerl , Yasmine Safraou , Rolf Reiter , Vanessa Proß , Kai Lehmann , Anja A. Kühl , Mehrgan Shahryari , Bernd Hamm , Ingolf Sack , Marcus R. Makowski , Jürgen Braun , Patrick Asbach\",\"doi\":\"10.1016/j.jmbbm.2024.106711\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Purpose</h3><p>To study the potential of viscoelastic parameters such as liver stiffness, loss tangent (marker of viscous properties) and viscoelastic dispersion to detect hepatic inflammation by <em>in-vivo</em> and <em>ex-vivo</em> MR elastography (MRE) at low and high vibration frequencies.</p></div><div><h3>Methods</h3><p>15 patients scheduled for liver tumor resection surgery were prospectively enrolled in this IRB-approved study and underwent multifrequency <em>in-vivo</em> MRE (30–60Hz) at 1.5-T prior to surgery. Immediately after liver resection, tumor-free tissue specimens were examined with <em>ex-vivo</em> MRE (0.8–2.8 kHz) at 0.5-T and histopathologic analysis including NAFLD activity score (NAS) and inflammation score (I-score) as sum of histological sub-features of inflammation.</p></div><div><h3>Results</h3><p><em>In-vivo</em><em>,</em> in regions where tissue samples were obtained, the loss tangent correlated with the I-score (R = 0.728; p = 0.002) and c-dispersion (stiffness dispersion over frequency) correlated with lobular inflammation (R = −0.559; p = 0.030). In a subgroup of patients without prior chemotherapy, c-dispersion correlated with I-score also in the whole liver (R = −0.682; p = 0.043). ROC analysis of the loss tangent for predicting the I-score showed a high AUC for I ≥ 1 (0.944; p = 0.021), I ≥ 2 (0.804; p = 0.049) and I ≥ 3 (0.944; p = 0.021). <em>Ex-vivo</em> MRE was not sensitive to inflammation, whereas strong correlations were observed between fibrosis and stiffness (R = 0.589; p = 0.021), penetration rate (R = 0.589; p = 0.021), loss tangent (R = −0.629; p = 0.012), and viscoelastic model parameters (spring-pot powerlaw exponent, R = −0.528; p = 0.043; spring-pot shear modulus, R = 0.589; p = 0.021).</p></div><div><h3>Conclusion</h3><p>Our results suggest that c-dispersion of the liver is sensitive to inflammation when measured <em>in-vivo</em> in the low dynamic range (30–60Hz), while at higher frequencies (0.8–2.8 kHz) viscoelastic parameters are dominated by fibrosis.</p></div>\",\"PeriodicalId\":380,\"journal\":{\"name\":\"Journal of the Mechanical Behavior of Biomedical Materials\",\"volume\":\"160 \",\"pages\":\"Article 106711\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2024-09-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1751616124003436/pdfft?md5=8c51f0462b02113837d352132b019949&pid=1-s2.0-S1751616124003436-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the Mechanical Behavior of Biomedical Materials\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1751616124003436\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Mechanical Behavior of Biomedical Materials","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1751616124003436","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 0
摘要
方法15名计划接受肝脏肿瘤切除手术的患者被前瞻性地纳入了这项经IRB批准的研究,并在手术前接受了1.5T的多频体内MRE(30-60Hz)检查。肝脏切除术后,立即在 0.5-T 下用体外 MRE(0.8-2.8 kHz)检查无肿瘤组织标本,并进行组织病理学分析,包括非酒精性脂肪肝活动评分(NAS)和炎症评分(I-score),作为炎症的组织学亚特征总和。结果在体内,在获得组织样本的区域,损耗正切与I-分数相关(R = 0.728; p = 0.002),c-分散(硬度随频率的分散)与小叶炎症相关(R = -0.559; p = 0.030)。在未接受过化疗的亚组患者中,c-分散度与全肝的 I-评分也有相关性(R = -0.682;p = 0.043)。预测 I 评分的损失切线 ROC 分析显示,I ≥ 1 (0.944; p = 0.021)、I ≥ 2 (0.804; p = 0.049) 和 I ≥ 3 (0.944; p = 0.021) 的 AUC 较高。体内外 MRE 对炎症不敏感,而在纤维化与硬度 (R = 0.589; p = 0.021)、穿透率 (R = 0.589; p = 0.021)、损耗正切 (R = -0.629; p = 0.012) 和粘弹性模型参数 (spring-pot powerlaw 指数,R = -0.结论我们的研究结果表明,在低动态范围(30-60Hz)内进行活体测量时,肝脏的 c-dispersion 对炎症很敏感,而在较高频率(0.8-2.8 kHz)下,粘弹性参数则受纤维化的影响。
Investigation of hepatic inflammation via viscoelasticity at low and high mechanical frequencies - A magnetic resonance elastography study
Purpose
To study the potential of viscoelastic parameters such as liver stiffness, loss tangent (marker of viscous properties) and viscoelastic dispersion to detect hepatic inflammation by in-vivo and ex-vivo MR elastography (MRE) at low and high vibration frequencies.
Methods
15 patients scheduled for liver tumor resection surgery were prospectively enrolled in this IRB-approved study and underwent multifrequency in-vivo MRE (30–60Hz) at 1.5-T prior to surgery. Immediately after liver resection, tumor-free tissue specimens were examined with ex-vivo MRE (0.8–2.8 kHz) at 0.5-T and histopathologic analysis including NAFLD activity score (NAS) and inflammation score (I-score) as sum of histological sub-features of inflammation.
Results
In-vivo, in regions where tissue samples were obtained, the loss tangent correlated with the I-score (R = 0.728; p = 0.002) and c-dispersion (stiffness dispersion over frequency) correlated with lobular inflammation (R = −0.559; p = 0.030). In a subgroup of patients without prior chemotherapy, c-dispersion correlated with I-score also in the whole liver (R = −0.682; p = 0.043). ROC analysis of the loss tangent for predicting the I-score showed a high AUC for I ≥ 1 (0.944; p = 0.021), I ≥ 2 (0.804; p = 0.049) and I ≥ 3 (0.944; p = 0.021). Ex-vivo MRE was not sensitive to inflammation, whereas strong correlations were observed between fibrosis and stiffness (R = 0.589; p = 0.021), penetration rate (R = 0.589; p = 0.021), loss tangent (R = −0.629; p = 0.012), and viscoelastic model parameters (spring-pot powerlaw exponent, R = −0.528; p = 0.043; spring-pot shear modulus, R = 0.589; p = 0.021).
Conclusion
Our results suggest that c-dispersion of the liver is sensitive to inflammation when measured in-vivo in the low dynamic range (30–60Hz), while at higher frequencies (0.8–2.8 kHz) viscoelastic parameters are dominated by fibrosis.
期刊介绍:
The Journal of the Mechanical Behavior of Biomedical Materials is concerned with the mechanical deformation, damage and failure under applied forces, of biological material (at the tissue, cellular and molecular levels) and of biomaterials, i.e. those materials which are designed to mimic or replace biological materials.
The primary focus of the journal is the synthesis of materials science, biology, and medical and dental science. Reports of fundamental scientific investigations are welcome, as are articles concerned with the practical application of materials in medical devices. Both experimental and theoretical work is of interest; theoretical papers will normally include comparison of predictions with experimental data, though we recognize that this may not always be appropriate. The journal also publishes technical notes concerned with emerging experimental or theoretical techniques, letters to the editor and, by invitation, review articles and papers describing existing techniques for the benefit of an interdisciplinary readership.