子宫内膜异位症妇女内脏脂肪组织褐变和炎症特征的证据

IF 4.7 3区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
José Pedro Abobeleira , Ana Catarina Neto , Jan Mauersberger , Maria Salazar , Maria Botelho , Ana Sofia Fernandes , Margarida Martinho , Maria Paula Serrão , Adriana Raquel Rodrigues , Henrique Almeida , Alexandra Maria Gouveia , Delminda Neves
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引用次数: 0

摘要

背景子宫内膜异位症患者的体重指数往往较低,这表明体脂与患病风险之间存在反比关系。有证据表明,在子宫内膜异位症中表达不同的 miRNA 在体外诱导前脂肪细胞棕色化,这也支持了上述观点。方法在子宫内膜异位症(32 例)或子宫肌瘤(14 例;对照组)手术期间采集内脏和皮下 AT 样本。血液儿茶酚胺采用高效液相色谱法测定,IL-6和TGF-β水平采用酶联免疫吸附法定量。通过计算机辅助形态测量法分析 H&E 染色切片中的脂肪细胞横截面积。组织中的巨噬细胞(F4/80;Galectin-3)和褐变活化(UCP-1;PGC-1α)通过双标记免疫荧光进行鉴定。通过 RT-PCR 和 Western 印迹法评估了 AT 中炎症(IL-6;MCP-1;Galectin-3;CD206;TIMP1;TGF-β)和褐变相关(UCP-1;PGC-1α;DIO2;CITED1;CIDEA;TMEM26;TBX1;PRDM16;PPAR-γ)分子的表达。患者血清中的 IL-6 增加,但 TGF-β 没有增加。与对照组相比,子宫内膜异位症妇女的增值肌肉中 UCP-1、PGC-1α、IL-6 和 MCP-1 上调,CD206 也减少。然而,IL-6、TIMP1 和 TGF-β 的 mRNA 表达以及 Galectin-3 蛋白水平均无差异。结论:我们的研究结果表明,子宫内膜异位症与 VAT 褐变和炎症的局部信号有关,是一种促代谢状态。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Evidence of Browning and Inflammation Features in Visceral Adipose Tissue of Women with Endometriosis

Evidence of Browning and Inflammation Features in Visceral Adipose Tissue of Women with Endometriosis

Background

Patients with endometriosis tend to have a low body mass index, suggesting an inverse relationship between body fat and risk of disease. This is supported by evidence that miRNAs differentially expressed in endometriosis induce browning of pre-adipocytes in vitro. Thus, we hypothesize that endometriosis may underlie adipose tissue (AT) dysfunction and browning.

Aims

Identify inflammation and browning processes in AT collected from endometriosis patients.

Methods

Visceral and subcutaneous AT samples were obtained during endometriosis (n = 32) or uterine myoma (n = 14; controls) surgery. Blood catecholamines were determined by high-performance liquid chromatography while IL-6 and TGF-β levels were quantified by ELISA. Adipocyte cross-sectional areas were analyzed in H&E-stained sections by computer-assisted morphometry. Macrophages (F4/80; Galectin-3) and browning activation (UCP-1; PGC-1α) in tissues were identified by dual label immunofluorescence. Expression of inflammatory (IL-6; MCP-1; Galectin-3; CD206; TIMP1; TGF-β) and browning-related (UCP-1; PGC-1α; DIO2; CITED1; CIDEA; TMEM26; TBX1; PRDM16; PPAR-γ) molecules in AT were assessed by RT-PCR and Western blotting.

Results

Compared to controls, patients presented smaller adipocytes, especially in VAT, and lower norepinephrine levels. Serum IL-6, but not TGF-β, was increased in patients. UCP-1, PGC-1α, IL-6, and MCP-1 were upregulated in VAT from endometriosis women, which also evidenced a reduction of CD206, relative to controls. However, no differences were found in mRNA expression of IL-6, TIMP1, and TGF-β nor Galectin-3 protein levels. In SAT, protein expression remained unchanged between patients and controls.

Conclusions

Our findings support an endometriosis’ role as a pro-catabolic state along with local signals of VAT browning and inflammation.

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来源期刊
Archives of Medical Research
Archives of Medical Research 医学-医学:研究与实验
CiteScore
12.50
自引率
0.00%
发文量
84
审稿时长
28 days
期刊介绍: Archives of Medical Research serves as a platform for publishing original peer-reviewed medical research, aiming to bridge gaps created by medical specialization. The journal covers three main categories - biomedical, clinical, and epidemiological contributions, along with review articles and preliminary communications. With an international scope, it presents the study of diseases from diverse perspectives, offering the medical community original investigations ranging from molecular biology to clinical epidemiology in a single publication.
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