Mingjin Fang , Yuan Yuan , Edward M. Fox , Ke Wu , Xin Tian , Likun Zhang , Hang Feng , Ruichao Li , Li Bai , Xinglong Wang , Zengqi Yang , Rong Zhang , Juan Wang
{"title":"携带 becAB 的产气荚膜梭菌菌株的流行率和基因组特征","authors":"Mingjin Fang , Yuan Yuan , Edward M. Fox , Ke Wu , Xin Tian , Likun Zhang , Hang Feng , Ruichao Li , Li Bai , Xinglong Wang , Zengqi Yang , Rong Zhang , Juan Wang","doi":"10.1016/j.fm.2024.104640","DOIUrl":null,"url":null,"abstract":"<div><p><em>Clostridium perfringens</em>, as a foodborne pathogen, can cause various intestinal diseases in both humans and animals according to its repertoire of toxins. In recent years, a multitude of studies have highlighted its threat to infants and young children. <em>C. perfringens</em> carries numerous toxins, with the newly identified BEC toxin confirmed as the second toxin to cause diarrheal illness, after CPE. However, the global dissemination of <em>C. perfringens</em> strains carrying <em>becAB</em> genes, which encode BEC toxins, has not been extensively studied. Following epidemiological surveillance of the prevalence of <em>C. perfringens</em> from different sources in various provinces of China, we identified two <em>becAB</em>-carrying strains and one strain carrying a sequence similar to <em>becAB</em> from distinct provinces and sources. When combined with genomic analysis of other <em>becAB</em>-carrying <em>C. perfringens</em> strains from public databases, we found that <em>becAB</em> was present in strains from different lineages. Our analysis of the plasmid and genetic environment corroborates previous findings on <em>becAB</em>-carrying strains, confirming that it currently achieves horizontal transmission through one type of evolutionarily conserved Pcp plasmid. This study provides a comprehensive analysis of the prevalence and transmission patterns of the newly emerged toxin gene locus, <em>becAB</em>, in <em>C. perfringens</em>. Despite the relatively low identification rate of <em>becAB</em>-carrying strains, their potential impact requires ongoing surveillance and investigation of their features, particularly their antimicrobial resistance.</p></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"125 ","pages":"Article 104640"},"PeriodicalIF":4.5000,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Prevalence and genomic characteristics of becAB-carrying Clostridium perfringens strains\",\"authors\":\"Mingjin Fang , Yuan Yuan , Edward M. Fox , Ke Wu , Xin Tian , Likun Zhang , Hang Feng , Ruichao Li , Li Bai , Xinglong Wang , Zengqi Yang , Rong Zhang , Juan Wang\",\"doi\":\"10.1016/j.fm.2024.104640\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Clostridium perfringens</em>, as a foodborne pathogen, can cause various intestinal diseases in both humans and animals according to its repertoire of toxins. In recent years, a multitude of studies have highlighted its threat to infants and young children. <em>C. perfringens</em> carries numerous toxins, with the newly identified BEC toxin confirmed as the second toxin to cause diarrheal illness, after CPE. However, the global dissemination of <em>C. perfringens</em> strains carrying <em>becAB</em> genes, which encode BEC toxins, has not been extensively studied. Following epidemiological surveillance of the prevalence of <em>C. perfringens</em> from different sources in various provinces of China, we identified two <em>becAB</em>-carrying strains and one strain carrying a sequence similar to <em>becAB</em> from distinct provinces and sources. When combined with genomic analysis of other <em>becAB</em>-carrying <em>C. perfringens</em> strains from public databases, we found that <em>becAB</em> was present in strains from different lineages. Our analysis of the plasmid and genetic environment corroborates previous findings on <em>becAB</em>-carrying strains, confirming that it currently achieves horizontal transmission through one type of evolutionarily conserved Pcp plasmid. This study provides a comprehensive analysis of the prevalence and transmission patterns of the newly emerged toxin gene locus, <em>becAB</em>, in <em>C. perfringens</em>. Despite the relatively low identification rate of <em>becAB</em>-carrying strains, their potential impact requires ongoing surveillance and investigation of their features, particularly their antimicrobial resistance.</p></div>\",\"PeriodicalId\":12399,\"journal\":{\"name\":\"Food microbiology\",\"volume\":\"125 \",\"pages\":\"Article 104640\"},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2024-09-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food microbiology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0740002024001783\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food microbiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0740002024001783","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Prevalence and genomic characteristics of becAB-carrying Clostridium perfringens strains
Clostridium perfringens, as a foodborne pathogen, can cause various intestinal diseases in both humans and animals according to its repertoire of toxins. In recent years, a multitude of studies have highlighted its threat to infants and young children. C. perfringens carries numerous toxins, with the newly identified BEC toxin confirmed as the second toxin to cause diarrheal illness, after CPE. However, the global dissemination of C. perfringens strains carrying becAB genes, which encode BEC toxins, has not been extensively studied. Following epidemiological surveillance of the prevalence of C. perfringens from different sources in various provinces of China, we identified two becAB-carrying strains and one strain carrying a sequence similar to becAB from distinct provinces and sources. When combined with genomic analysis of other becAB-carrying C. perfringens strains from public databases, we found that becAB was present in strains from different lineages. Our analysis of the plasmid and genetic environment corroborates previous findings on becAB-carrying strains, confirming that it currently achieves horizontal transmission through one type of evolutionarily conserved Pcp plasmid. This study provides a comprehensive analysis of the prevalence and transmission patterns of the newly emerged toxin gene locus, becAB, in C. perfringens. Despite the relatively low identification rate of becAB-carrying strains, their potential impact requires ongoing surveillance and investigation of their features, particularly their antimicrobial resistance.
期刊介绍:
Food Microbiology publishes original research articles, short communications, review papers, letters, news items and book reviews dealing with all aspects of the microbiology of foods. The editors aim to publish manuscripts of the highest quality which are both relevant and applicable to the broad field covered by the journal. Studies must be novel, have a clear connection to food microbiology, and be of general interest to the international community of food microbiologists. The editors make every effort to ensure rapid and fair reviews, resulting in timely publication of accepted manuscripts.