不同细菌和真菌共培养物对木质纤维素的降解作用

IF 4.8 Q1 MICROBIOLOGY
Julian Detain, Ludovic Besaury
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引用次数: 0

摘要

长期以来,农业副产品一直被视为不可估价的废物,但现在却越来越多地被用于生物精炼工艺中。共培养成为改善木质纤维素降解和提高生物产品产量的新趋势。本研究的目标是采用生物信息学、酶学和转录组学相结合的多学科方法,建立具有较高木质纤维素降解能力的跨域共培养物。不同的木质纤维素分解菌株:该研究使用了不同的木质纤维素分解菌株:Trichoderma reesei QM6a 和三种细菌(来自不同门的 Streptomyces coelicolor A3(2)、Rhizobium sp.XylPr11 和 Sphingobacterium prati AraPr2)。在共培养条件下观察到并量化了协同活性,尤其是木聚糖酶和过氧化物酶活性。在最有趣的共培养(T. reesei QM6a/S. coelicolor A3(2))中,木聚糖酶和过氧化物酶的酶活性分别高达 2 IU/mL 和 430 IU/mL。此外,ATR-傅立叶变换红外分析表明,与单培养相比,共培养条件对底物有真正的影响,特别是在半纤维素降解方面。无论是单培养还是共培养,S. coelicolor A3(2)的转录组学都显示出相对相似的模式特征,无论分析的条件如何,由于真菌伙伴发挥的水解作用,某些参与糖酵解的 CAZyme 基因都会出现特定的过表达。这项工作证明了跨域共培养的技术可行性、相关性和实用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Degradation of lignocellulose by different bacterial and fungal co-cultures

Degradation of lignocellulose by different bacterial and fungal co-cultures

Long seen as non-valorisable waste, agricultural co-products are increasingly used in biorefinery processes. Co-culture appears as new trend for to improve the degradation of lignocellulose and improve the production of bioproducts. The goal of the study was to setup inter-domain co-cultures with high capabilities of lignocellulose degradation using a pluridisciplinary approach combining bioinformatics, enzymology, transcriptomics. Different individual lignocellulolytic strains: Trichoderma reesei QM6a and three bacteria (Streptomyces coelicolor A3(2), Rhizobium sp.XylPr11 and Sphingobacterium prati AraPr2 affiliated from different phyla) were used in that study . Synergic activities have been observed and quantified in co-culture conditions, particularly for xylanases and peroxidases activities. The enzymatic activities for the co-cultures in the most interesting co-culture (T. reesei QM6a/S. coelicolor A3(2)) reached more up to 2 IU/mL and 430 IU/mL respectively for the xylanase and peroxidase. Furthermore, ATR-FTIR analysis showed a real impact of co-culture condition on the substrate compared to the monoculture specially for hemicellulose degradation. Transcriptomics of S. coelicolor A3(2) either in mono or co-culture showed a relative similar pattern profile whatever the condition analysed with a specific overexpression of certain CAZyme genes involved in glycolysis due to the hydrolytic role played by the fungal partner. This work provided the proof of concept for technological feasibility, pertinence and usefulness of interdomain co-culture.

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来源期刊
Current Research in Microbial Sciences
Current Research in Microbial Sciences Immunology and Microbiology-Immunology and Microbiology (miscellaneous)
CiteScore
7.90
自引率
0.00%
发文量
81
审稿时长
66 days
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