不同细菌和真菌共培养物对木质纤维素的降解作用

IF 4.8 Q1 MICROBIOLOGY
{"title":"不同细菌和真菌共培养物对木质纤维素的降解作用","authors":"","doi":"10.1016/j.crmicr.2024.100271","DOIUrl":null,"url":null,"abstract":"<div><p>Long seen as non-valorisable waste, agricultural co-products are increasingly used in biorefinery processes. Co-culture appears as new trend for to improve the degradation of lignocellulose and improve the production of bioproducts. The goal of the study was to setup inter-domain co-cultures with high capabilities of lignocellulose degradation using a pluridisciplinary approach combining bioinformatics, enzymology, transcriptomics. Different individual lignocellulolytic strains: <em>Trichoderma reesei</em> QM6a and three bacteria (<em>Streptomyces coelicolor</em> A3(2), <em>Rhizobium</em> sp.XylPr11 and <em>Sphingobacterium prati</em> AraPr2 affiliated from different phyla) were used in that study . Synergic activities have been observed and quantified in co-culture conditions, particularly for xylanases and peroxidases activities. The enzymatic activities for the co-cultures in the most interesting co-culture (<em>T. reesei QM6a/S. coelicolor A3(2)</em>) reached more up to 2 IU/mL and 430 IU/mL respectively for the xylanase and peroxidase. Furthermore, ATR-FTIR analysis showed a real impact of co-culture condition on the substrate compared to the monoculture specially for hemicellulose degradation. Transcriptomics of <em>S. coelicolor</em> A3(2) either in mono or co-culture showed a relative similar pattern profile whatever the condition analysed with a specific overexpression of certain CAZyme genes involved in glycolysis due to the hydrolytic role played by the fungal partner. This work provided the proof of concept for technological feasibility, pertinence and usefulness of interdomain co-culture.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":null,"pages":null},"PeriodicalIF":4.8000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000531/pdfft?md5=3affb225bcb8fb63efcf3efe4560a02c&pid=1-s2.0-S2666517424000531-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Degradation of lignocellulose by different bacterial and fungal co-cultures\",\"authors\":\"\",\"doi\":\"10.1016/j.crmicr.2024.100271\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Long seen as non-valorisable waste, agricultural co-products are increasingly used in biorefinery processes. Co-culture appears as new trend for to improve the degradation of lignocellulose and improve the production of bioproducts. The goal of the study was to setup inter-domain co-cultures with high capabilities of lignocellulose degradation using a pluridisciplinary approach combining bioinformatics, enzymology, transcriptomics. Different individual lignocellulolytic strains: <em>Trichoderma reesei</em> QM6a and three bacteria (<em>Streptomyces coelicolor</em> A3(2), <em>Rhizobium</em> sp.XylPr11 and <em>Sphingobacterium prati</em> AraPr2 affiliated from different phyla) were used in that study . Synergic activities have been observed and quantified in co-culture conditions, particularly for xylanases and peroxidases activities. The enzymatic activities for the co-cultures in the most interesting co-culture (<em>T. reesei QM6a/S. coelicolor A3(2)</em>) reached more up to 2 IU/mL and 430 IU/mL respectively for the xylanase and peroxidase. Furthermore, ATR-FTIR analysis showed a real impact of co-culture condition on the substrate compared to the monoculture specially for hemicellulose degradation. Transcriptomics of <em>S. coelicolor</em> A3(2) either in mono or co-culture showed a relative similar pattern profile whatever the condition analysed with a specific overexpression of certain CAZyme genes involved in glycolysis due to the hydrolytic role played by the fungal partner. This work provided the proof of concept for technological feasibility, pertinence and usefulness of interdomain co-culture.</p></div>\",\"PeriodicalId\":34305,\"journal\":{\"name\":\"Current Research in Microbial Sciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2666517424000531/pdfft?md5=3affb225bcb8fb63efcf3efe4560a02c&pid=1-s2.0-S2666517424000531-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Research in Microbial Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2666517424000531\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Research in Microbial Sciences","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666517424000531","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

长期以来,农业副产品一直被视为不可估价的废物,但现在却越来越多地被用于生物精炼工艺中。共培养成为改善木质纤维素降解和提高生物产品产量的新趋势。本研究的目标是采用生物信息学、酶学和转录组学相结合的多学科方法,建立具有较高木质纤维素降解能力的跨域共培养物。不同的木质纤维素分解菌株:该研究使用了不同的木质纤维素分解菌株:Trichoderma reesei QM6a 和三种细菌(来自不同门的 Streptomyces coelicolor A3(2)、Rhizobium sp.XylPr11 和 Sphingobacterium prati AraPr2)。在共培养条件下观察到并量化了协同活性,尤其是木聚糖酶和过氧化物酶活性。在最有趣的共培养(T. reesei QM6a/S. coelicolor A3(2))中,木聚糖酶和过氧化物酶的酶活性分别高达 2 IU/mL 和 430 IU/mL。此外,ATR-傅立叶变换红外分析表明,与单培养相比,共培养条件对底物有真正的影响,特别是在半纤维素降解方面。无论是单培养还是共培养,S. coelicolor A3(2)的转录组学都显示出相对相似的模式特征,无论分析的条件如何,由于真菌伙伴发挥的水解作用,某些参与糖酵解的 CAZyme 基因都会出现特定的过表达。这项工作证明了跨域共培养的技术可行性、相关性和实用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Degradation of lignocellulose by different bacterial and fungal co-cultures

Degradation of lignocellulose by different bacterial and fungal co-cultures

Long seen as non-valorisable waste, agricultural co-products are increasingly used in biorefinery processes. Co-culture appears as new trend for to improve the degradation of lignocellulose and improve the production of bioproducts. The goal of the study was to setup inter-domain co-cultures with high capabilities of lignocellulose degradation using a pluridisciplinary approach combining bioinformatics, enzymology, transcriptomics. Different individual lignocellulolytic strains: Trichoderma reesei QM6a and three bacteria (Streptomyces coelicolor A3(2), Rhizobium sp.XylPr11 and Sphingobacterium prati AraPr2 affiliated from different phyla) were used in that study . Synergic activities have been observed and quantified in co-culture conditions, particularly for xylanases and peroxidases activities. The enzymatic activities for the co-cultures in the most interesting co-culture (T. reesei QM6a/S. coelicolor A3(2)) reached more up to 2 IU/mL and 430 IU/mL respectively for the xylanase and peroxidase. Furthermore, ATR-FTIR analysis showed a real impact of co-culture condition on the substrate compared to the monoculture specially for hemicellulose degradation. Transcriptomics of S. coelicolor A3(2) either in mono or co-culture showed a relative similar pattern profile whatever the condition analysed with a specific overexpression of certain CAZyme genes involved in glycolysis due to the hydrolytic role played by the fungal partner. This work provided the proof of concept for technological feasibility, pertinence and usefulness of interdomain co-culture.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Current Research in Microbial Sciences
Current Research in Microbial Sciences Immunology and Microbiology-Immunology and Microbiology (miscellaneous)
CiteScore
7.90
自引率
0.00%
发文量
81
审稿时长
66 days
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信