HPV16阳性口咽癌中的lncRNA和miRNA调控轴

IF 2.8 3区 医学 Q3 VIROLOGY
Dayna Sais , Meredith Hill , Fiona Deutsch , Phuong Thao Nguyen , Valerie Gay , Nham Tran
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引用次数: 0

摘要

与人类乳头瘤病毒(HPV)16型相关的口咽癌(OPC)在全球范围内呈上升趋势,因此有必要深入了解其潜在的分子机制。我们的研究利用癌症基因组图谱(TCGA)中的RNA测序数据,鉴定和分析了HPV16阳性口咽癌患者体内差异表达(DE)的长非编码RNA(lncRNA)、微RNA(miRNA)和信使RNA(mRNA),并阐明了lncRNA/miRNA/mRNA调控网络中的相互作用。我们发现了 1929 个 DE lncRNA,并确定了其中 37 个的显著表达变化,这表明 miRNA 具有调控细胞过程的 "海绵 "功能。值得注意的是,lncRNA Linc00911在HPV16阳性OPC中的表达量减少,这一变化可直接归因于HPV致癌基因E6和E7,细胞系和患者样本中的RT-qPCR证实了这一点。我们的综合分析展示了 ncRNA 与 MRNA 相互作用的广阔前景,为不断探索阐明 HPV 驱动的 OPC 的分子基础提供了资源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The lncRNA and miRNA regulatory axis in HPV16-positive oropharyngeal cancers

The global rise of oropharyngeal cancers (OPC) associated with the human papillomavirus (HPV) type 16 necessitates a deeper understanding of their underlying molecular mechanisms. Our study utilised RNA-sequencing data from The Cancer Genome Atlas (TCGA) to identify and analyse differentially expressed (DE) long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs) in HPV16-positive OPC, and to elucidate the interplay within the lncRNA/miRNA/mRNA regulatory network. We revealed 1929 DE lncRNAs and identified a significant expression shift in 37 of these, suggesting a regulatory 'sponge' function for miRNAs that modulate cellular processes. Notably, the lncRNA Linc00911 exhibited decreased expression in HPV16-positive OPC, a change directly attributable to HPV oncogenes E6 and E7 as confirmed by RT-qPCR in cell lines and patient samples. Our comprehensive analysis presents an expansive landscape of ncRNA-mRNA interactions, offering a resource for the ongoing pursuit of elucidating the molecular underpinnings of HPV-driven OPC.

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来源期刊
Virology
Virology 医学-病毒学
CiteScore
6.00
自引率
0.00%
发文量
157
审稿时长
50 days
期刊介绍: Launched in 1955, Virology is a broad and inclusive journal that welcomes submissions on all aspects of virology including plant, animal, microbial and human viruses. The journal publishes basic research as well as pre-clinical and clinical studies of vaccines, anti-viral drugs and their development, anti-viral therapies, and computational studies of virus infections. Any submission that is of broad interest to the community of virologists/vaccinologists and reporting scientifically accurate and valuable research will be considered for publication, including negative findings and multidisciplinary work.Virology is open to reviews, research manuscripts, short communication, registered reports as well as follow-up manuscripts.
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