Jin A Kim , Young-Su Park , Jun-Hwan Kim , Cheol Young Choi
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The activities of antioxidant enzymes, specifically SOD and CAT, in <em>C. notata</em> exposed to low and high temperatures, showed an increase compared to the control group (maintained at 18 °C). Moreover, liver H<sub>2</sub>O<sub>2</sub> levels exhibited a significant increase over time. Conversely, plasma lysozyme activity significantly decreased in groups subjected to low and high water temperatures compared to the control group. Analyzing changes in the intestinal microbiota, we observed an increase in the proportion of Firmicutes but a decrease in Proteobacteria, which are known for their role in immune enhancement, in <em>C. notata</em> exposed to both low and high water temperatures. We propose that alterations in water temperature impact the antioxidant enzyme activity of <em>C. notata</em>, leading to compromised immune responses and disruption of the biological balance of the intestinal microbiota, potentially affecting the host's survival.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"275 ","pages":"Article 111029"},"PeriodicalIF":1.8000,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Impact of water temperature on oxidative stress and intestinal microbiota in pearl-spot chromis, Chromis notata (Temminck & Schlegel, 1843)\",\"authors\":\"Jin A Kim , Young-Su Park , Jun-Hwan Kim , Cheol Young Choi\",\"doi\":\"10.1016/j.cbpb.2024.111029\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Water temperature is an abiotic factor influencing fish metabolism and physiological responses. As poikilothermic creatures, fish are notable sensitivity to fluctuations in water temperature, which also significantly influences intestinal microbial proliferation. This study aimed to investigate the impact of both low (8 °C) and high (28 °C) water temperatures on oxidative stress and the intestinal microbiota of <em>Chromis notata</em>, a species that has recently migrated northward owing to changes in sea water temperature. Laboratory experiments were conducted to assess changes in superoxide dismutase (SOD), catalase (CAT), and lysozyme activities, as well as changes in the abundance and diversity of intestinal microbiota. The activities of antioxidant enzymes, specifically SOD and CAT, in <em>C. notata</em> exposed to low and high temperatures, showed an increase compared to the control group (maintained at 18 °C). Moreover, liver H<sub>2</sub>O<sub>2</sub> levels exhibited a significant increase over time. Conversely, plasma lysozyme activity significantly decreased in groups subjected to low and high water temperatures compared to the control group. Analyzing changes in the intestinal microbiota, we observed an increase in the proportion of Firmicutes but a decrease in Proteobacteria, which are known for their role in immune enhancement, in <em>C. notata</em> exposed to both low and high water temperatures. We propose that alterations in water temperature impact the antioxidant enzyme activity of <em>C. notata</em>, leading to compromised immune responses and disruption of the biological balance of the intestinal microbiota, potentially affecting the host's survival.</p></div>\",\"PeriodicalId\":55236,\"journal\":{\"name\":\"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology\",\"volume\":\"275 \",\"pages\":\"Article 111029\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-09-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1096495924000964\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1096495924000964","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
水温是影响鱼类新陈代谢和生理反应的非生物因素。鱼类作为变温动物,对水温波动表现出明显的敏感性,而水温波动也会显著影响肠道微生物的增殖。本研究旨在调查低水温(8 °C)和高水温(28 °C)对鲑鱼氧化应激和肠道微生物群的影响。实验室实验评估了超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和溶菌酶活性的变化,以及肠道微生物群丰度和多样性的变化。与对照组(保持在 18 °C)相比,暴露于低温和高温下的 C. notata 的抗氧化酶(特别是 SOD 和 CAT)活性有所提高。此外,肝脏中的 H2O2 含量也随着时间的推移而显著增加。相反,与对照组相比,低水温组和高温组的血浆溶菌酶活性显著降低。在分析肠道微生物群的变化时,我们观察到,在暴露于低水温和高水温的 C. notata 中,固着菌的比例增加,而蛋白质细菌的比例下降,而蛋白质细菌在免疫增强中的作用是众所周知的。我们认为,水温的变化会影响 C. notata 的抗氧化酶活性,导致免疫反应受损和肠道微生物群的生物平衡被破坏,从而可能影响宿主的生存。
Impact of water temperature on oxidative stress and intestinal microbiota in pearl-spot chromis, Chromis notata (Temminck & Schlegel, 1843)
Water temperature is an abiotic factor influencing fish metabolism and physiological responses. As poikilothermic creatures, fish are notable sensitivity to fluctuations in water temperature, which also significantly influences intestinal microbial proliferation. This study aimed to investigate the impact of both low (8 °C) and high (28 °C) water temperatures on oxidative stress and the intestinal microbiota of Chromis notata, a species that has recently migrated northward owing to changes in sea water temperature. Laboratory experiments were conducted to assess changes in superoxide dismutase (SOD), catalase (CAT), and lysozyme activities, as well as changes in the abundance and diversity of intestinal microbiota. The activities of antioxidant enzymes, specifically SOD and CAT, in C. notata exposed to low and high temperatures, showed an increase compared to the control group (maintained at 18 °C). Moreover, liver H2O2 levels exhibited a significant increase over time. Conversely, plasma lysozyme activity significantly decreased in groups subjected to low and high water temperatures compared to the control group. Analyzing changes in the intestinal microbiota, we observed an increase in the proportion of Firmicutes but a decrease in Proteobacteria, which are known for their role in immune enhancement, in C. notata exposed to both low and high water temperatures. We propose that alterations in water temperature impact the antioxidant enzyme activity of C. notata, leading to compromised immune responses and disruption of the biological balance of the intestinal microbiota, potentially affecting the host's survival.
期刊介绍:
Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology.
Part B: Biochemical and Molecular Biology (CBPB), focuses on biochemical physiology, primarily bioenergetics/energy metabolism, cell biology, cellular stress responses, enzymology, intermediary metabolism, macromolecular structure and function, gene regulation, evolutionary genetics. Most studies focus on biochemical or molecular analyses that have clear ramifications for physiological processes.