对第 2.5-3 天的牛胚胎进行热休克,可挑选出最有能力进入囊胚期的胚胎。

IF 2.4 2区 农林科学 Q3 REPRODUCTIVE BIOLOGY
Luciano de Rezende Carvalheira , Ana Carolina Leite Albeny, Eliane Beatriz Magalhães Silva, Álan Maia Borges
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引用次数: 0

摘要

热休克会损害胚胎的形成,而生长因子(如集落刺激因子 2 (CSF2))则会调节胚胎的发育。本研究评估了热休克在第 2.5 天和第 3 天之间的影响,以及 CSF2 在第 5 天对在无血清体外培养基中培养的牛胚胎的影响。重点是囊胚发育、囊胚体数量、DNA 断裂(TUNEL 阳性细胞)和线粒体活性。热休克降低了发育成囊胚的裂解胚胎比例(P = 0.0603)。由此产生的囊胚在滋养层(P = 0.0270 和 P = 0.0240)和整个胚胎(P = 0.0029 和 P = 0.0031)中表现出 TUNEL 阳性细胞的数量和比例减少。此外,热休克胚胎产生的囊胚中线粒体活性较低(P = 0.0150),同时暴露于热休克和 CSF2 的胚胎中线粒体活性进一步降低(P = 0.0415)。总之,将裂解胚胎暴露于热休克会减少其发育至囊胚阶段。然而,由此产生的囊胚显示出 DNA 断裂和线粒体活性降低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Heat shock on bovine embryos from day 2.5–3 selects the most competent for progression to the blastocyst stage

Heat shock can impair embryo formation, while growth factors, such as colony-stimulating factor 2 (CSF2), modulate embryonic development. This study evaluated the effect of heat shock between days 2.5 and 3, as well as the impact of CSF2 at day 5 on bovine embryos cultured in a serum-free in vitro medium. The focus was on blastocyst development, the number of blastomeres, DNA fragmentation (TUNEL-positive cells), and mitochondrial activity. Heat shock reduced the proportion of cleaved embryos that developed into blastocysts (P = 0.0603). The resultant blastocysts exhibited a reduced number and proportion of TUNEL-positive cells in the trophectoderm (P = 0.0270 and P = 0.0240, respectively) and in the entire embryo (P = 0.0029 and P = 0.0031, respectively). Additionally, mitochondrial activity was lower in blastocysts derived from heat-shocked embryos (P = 0.0150) and further reduced in embryos exposed to both heat shock and CSF2 (P = 0.0415). In conclusion, the exposure of cleaved embryos to heat shock reduced their development to the blastocyst stage. However, the resulting blastocysts showed decreased DNA fragmentation and mitochondrial activity.

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来源期刊
Theriogenology
Theriogenology 农林科学-生殖生物学
CiteScore
5.50
自引率
14.30%
发文量
387
审稿时长
72 days
期刊介绍: Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.
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