利用 PSF 分光技术同时进行多色荧光成像。

IF 36.1 1区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Nature Methods Pub Date : 2024-09-06 DOI:10.1038/s41592-024-02383-7

Robin Van den Eynde, Fabian Hertel, Sergey Abakumov, Bartosz Krajnik, Siewert Hugelier, Alexander Auer, Joschka Hellmeier, Thomas Schlichthaerle, Rachel M. Grattan, Diane S. Lidke, Ralf Jungmann, Marcel Leutenegger, Wim Vandenberg, Peter Dedecker

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引用次数: 0

摘要

我们提出了一种通过分割原始点扩散函数（PSF）在荧光成像中编码更多信息的方法，这种方法可提供宽带操作，并与其他 PSF 工程模式和现有分析工具兼容。我们使用 "Circulator "演示了这种方法，它是一种将荧光团发射带编码到 PSF 中的附加装置，可同时使用全视场进行多色超分辨率和单分子显微镜观察。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Simultaneous multicolor fluorescence imaging using PSF splitting

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Simultaneous multicolor fluorescence imaging using PSF splitting

We present a way to encode more information in fluorescence imaging by splitting the original point spread function (PSF), which offers broadband operation and compatibility with other PSF engineering modalities and existing analysis tools. We demonstrate the approach using the ‘Circulator’, an add-on that encodes the fluorophore emission band into the PSF, enabling simultaneous multicolor super-resolution and single-molecule microscopy using essentially the full field of view. Point spread function (PSF) splitting with the ‘Circulator’, which encodes the fluorophore emission band into the PSF, improves the information content of fluorescence microscopy and enables improved super-resolution imaging and single-particle tracking.

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来源期刊

Nature Methods 生物-生化研究方法

CiteScore

58.70

自引率

1.70%

发文量

326

审稿时长

1 months

期刊介绍： Nature Methods is a monthly journal that focuses on publishing innovative methods and substantial enhancements to fundamental life sciences research techniques. Geared towards a diverse, interdisciplinary readership of researchers in academia and industry engaged in laboratory work, the journal offers new tools for research and emphasizes the immediate practical significance of the featured work. It publishes primary research papers and reviews recent technical and methodological advancements, with a particular interest in primary methods papers relevant to the biological and biomedical sciences. This includes methods rooted in chemistry with practical applications for studying biological problems.