WSS15 醋酸酵母菌膜囊部分的特征。

IF 2.3 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of bioscience and bioengineering Pub Date : 2024-09-05 DOI:10.1016/j.jbiosc.2024.07.017

Atsushi Kurata , Kota Aimatsu , Yuki Kimura , Hinako Hashiguchi , Asami Maeda , Tomoya Imai , Shino Yamasaki-Yashiki , Kensaku Hamada , Yuki Fujimoto , Akira Fujii , Koichi Uegaki

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引用次数: 0

摘要

从日本一种名为 Kurozu 的传统醋中分离出了一种能产生膜囊泡 (MV) 的细菌--菌株 WSS15。16S rRNA 基因序列的系统进化分析表明，该细菌属于醋杆菌属。在菌株 WSS15 的噬菌体部分中检测到了噬菌体和肽聚糖相关脂蛋白（Pal）。在有 WSS15 菌株中空菌体部分存在的情况下，小鼠巨噬细胞会通过表层 Toll 样受体 2（TLR2）的识别产生促炎细胞因子白细胞介素-6（IL-6）。WSS15 MVs 吸附在巨噬细胞的细胞表面。巨噬细胞通过 TLR2 识别 Pal 的酰化 N 端肽分泌 IL-6。我们阐明了 WSS15 MVs 对免疫细胞的作用模式，并确定菌株 WSS15 的 Pal 肽是 TLR2 的激动剂。

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Characterization of the membrane vesicle fraction from Acetobacter sp. WSS15

A bacterium that produces membrane vesicles (MVs), strain WSS15, was isolated from a traditional vinegar in Japan called Kurozu. A phylogenetic analysis of 16S rRNA gene sequences indicated that this bacterium belongs to the genus Acetobacter. MVs and peptidoglycan-associated lipoprotein (Pal) were detected in the MV fraction of strain WSS15. In the presence of the WSS15 MV fraction, murine macrophages produced the pro-inflammatory cytokine interleukin-6 (IL-6) via the recognition by superficial Toll-like receptor 2 (TLR2). WSS15 MVs adhered to the cell surface of macrophages. The macrophages secreted IL-6 through the TLR2 recognition of an acylated N-terminal peptide of Pal. We elucidated the mode of action of WSS15 MVs on immune cells and identified the Pal peptide from strain WSS15 as an agonist of TLR2.

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来源期刊

Journal of bioscience and bioengineering 生物-生物工程与应用微生物

CiteScore

5.90

自引率

3.60%

发文量

144

审稿时长

51 days

期刊介绍： The Journal of Bioscience and Bioengineering is a research journal publishing original full-length research papers, reviews, and Letters to the Editor. The Journal is devoted to the advancement and dissemination of knowledge concerning fermentation technology, biochemical engineering, food technology and microbiology.