利用微盐梯度辅助固态纳米孔高灵敏检测肿瘤细胞衍生的外泌体

IF 8.2 2区 材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY
ACS Applied Materials & Interfaces Pub Date : 2024-09-18 Epub Date: 2024-09-06 DOI:10.1021/acsami.4c14224
Libo Zhu, Zhengyuan Xu, Yanfeng Gao, Na Sun, Lei Qiu, Jinsong Zhao
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引用次数: 0

摘要

作为一种重要的生物标志物,肿瘤细胞衍生的外泌体在癌症早期筛查和诊断中具有广阔的应用前景。然而,传统检测方法灵敏度不高、样品前处理过程复杂,限制了其在临床诊断中的应用。纳米孔传感器作为一种高灵敏度、无标记的单分子技术,被广泛应用于分子和生物颗粒的检测。然而,由于外泌体表面电荷密度较低,且电解质浓度会影响其结构稳定性,因此通过纳米孔捕获外泌体的速率极低,从而降低了检测通量。在此,我们报告了一种利用氮化硅(SiNx)纳米孔在轻微的盐电解质梯度辅助下提高外泌体转位捕获率的方法。在不同浓度梯度的电解质溶液中,我们评估了外泌体易位事件频率的提高情况。在不对称电解质(顺式1×PBS和反式0.2 M NaCl,1×PBS)中,肿瘤细胞(HepG2)产生的外泌体转运事件频率提高了近2个数量级,同时保持了囊泡结构的稳定性。此外,得益于盐梯度效应,肿瘤细胞(AsPC-1和HCT116)衍生的外泌体易位与HepG2细胞衍生的外泌体易位可以区分开来。所开发的单颗粒水平肿瘤细胞外泌体高灵敏度检测方法为早期癌症诊断提供了一种方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Highly Sensitive Detection of Tumor Cell-Derived Exosomes Using Solid-State Nanopores Assisted with a Slight Salt Gradient.

Highly Sensitive Detection of Tumor Cell-Derived Exosomes Using Solid-State Nanopores Assisted with a Slight Salt Gradient.

As an important biomarker, tumor cell-derived exosomes have substantial application prospects in early cancer screening and diagnosis. However, the unsatisfactory sensitivity and complicated sample pretreatment processes of conventional detection approaches have limited their use in clinical diagnosis. Nanopore sensors, as a highly sensitive, label-free, single-molecule technology, are widely utilized in molecule and bioparticle detection. Nevertheless, the exosome capture rate through nanopores is extremely low due to the low surface charge densities of exosomes and the effects of electrolyte concentration on their structural stability, thereby reducing the detection throughput. Here, we report an approach to improve the capture rate of exosome translocations using silicon nitride (SiNx) nanopores assisted by a slight salt electrolyte gradient. Improvements in exosome translocation event frequency are assessed in electrolyte solutions with different concentration gradients. In the case of asymmetric electrolytes (cis1× PBS and trans0.2 M NaCl, 1× PBS), the event frequency of tumor cell (HepG2)-derived exosome translocations is enhanced by nearly 2 orders of magnitude while maintaining vesicle structure stability. Furthermore, benefiting from the salt gradient effect, tumor cell (AsPC-1 and HCT116)-derived exosome translocations could be discriminated from those of HepG2 cell-derived exosomes. The developed highly sensitive detection method for tumor cell-derived exosomes at the single-particle level provides an approach for early cancer diagnosis.

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来源期刊
ACS Applied Materials & Interfaces
ACS Applied Materials & Interfaces 工程技术-材料科学:综合
CiteScore
16.00
自引率
6.30%
发文量
4978
审稿时长
1.8 months
期刊介绍: ACS Applied Materials & Interfaces is a leading interdisciplinary journal that brings together chemists, engineers, physicists, and biologists to explore the development and utilization of newly-discovered materials and interfacial processes for specific applications. Our journal has experienced remarkable growth since its establishment in 2009, both in terms of the number of articles published and the impact of the research showcased. We are proud to foster a truly global community, with the majority of published articles originating from outside the United States, reflecting the rapid growth of applied research worldwide.
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