来自金黄色葡萄球菌的α-溶血素改变了 Th17 细胞的表观遗传景观

Q3 Medicine
Joanna Pastwińska, Iwona Karwaciak, Kaja Karaś, Anna Sałkowska, Katarzyna Chałaśkiewicz, Dominik Strapagiel, Marta Sobalska-Kwapis, Jarosław Dastych, Marcin Ratajewski
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引用次数: 0

摘要

人体内有大量细菌,其数量可能超过宿主细胞。因此,两种细胞类型之间存在多种相互作用。鉴于人体内普遍存在金黄色葡萄球菌,而 Th17 细胞在控制粘膜上的这种病原体方面发挥作用,我们试图研究这种细菌产生的 α 溶血素对分化的 Th17 细胞的影响。RNA测序分析表明,α-溶血素会影响Th17细胞特征基因以及参与表观遗传调控基因的表达。我们通过全基因组亚硫酸氢盐测序观察到了各种组蛋白标记和基因组甲基化水平的改变。我们的研究结果强调了细菌蛋白如何显著影响人类 Th17 细胞的转录组、表观基因组和表型,突出了免疫细胞与微生物群之间相互作用的错综复杂性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
α-Hemolysin from Staphylococcus aureus Changes the Epigenetic Landscape of Th17 Cells.

The human body harbors a substantial population of bacteria, which may outnumber host cells. Thus, there are multiple interactions between both cell types. Given the common presence of Staphylococcus aureus in the human body and the role of Th17 cells in controlling this pathogen on mucous membranes, we sought to investigate the effect of α-hemolysin, which is produced by this bacterium, on differentiating Th17 cells. RNA sequencing analysis revealed that α-hemolysin influences the expression of signature genes for Th17 cells as well as genes involved in epigenetic regulation. We observed alterations in various histone marks and genome methylation levels via whole-genome bisulfite sequencing. Our findings underscore how bacterial proteins can significantly influence the transcriptome, epigenome, and phenotype of human Th17 cells, highlighting the intricate and complex nature of the interaction between immune cells and the microbiota.

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CiteScore
3.70
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