AtALMT5 在拟南芥中介导液泡富马酸的输入并调节苹果酸/富马酸的平衡。

IF 8.3 1区 生物学 Q1 PLANT SCIENCES
New Phytologist Pub Date : 2024-09-05 DOI:10.1111/nph.20077
Roxane Doireau, Justyna Jaślan, Paloma Cubero-Font, Elsa Demes-Causse, Karen Bertaux, Cédric Cassan, Pierre Pétriacq, Alexis De Angeli
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引用次数: 0

摘要

苹果酸盐和富马酸盐占光合作用固定碳的很大一部分,它们处于中央代谢途径的十字路口。在拟南芥中,它们被暂时储存在液泡中,以保持细胞平衡。液泡膜上的苹果酸和富马酸转运系统是控制细胞代谢的关键角色。值得注意的是,这些转运系统的分子特性大部分仍未得到解决。我们将成像、电生理学和分子生理学结合起来,确定了二羧酸跨滋养层转运的一个重要分子角色。在此,我们报告了大连蛛铝激活的苹果酸转运体 5(AtALMT5)的功能。我们研究了它在体内的离子转运特性、表达模式、定位和功能。我们的研究表明,AtALMT5 在光合作用活跃的组织中表达,并定位在调质体中。膜片钳和植物体分析表明,AtALMT5 是一种离子通道,介导液泡中富马酸盐的负载。我们发现在 almt5 植物中,叶片中富马酸的积累减少,与此同时苹果酸的浓度增加。这些结果确定了 AtALMT5 是一种离子通道,它介导叶肉细胞液泡中的富马酸盐运输,并调节拟南芥中苹果酸盐/富马酸盐的平衡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

AtALMT5 mediates vacuolar fumarate import and regulates the malate/fumarate balance in Arabidopsis

AtALMT5 mediates vacuolar fumarate import and regulates the malate/fumarate balance in Arabidopsis

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来源期刊
New Phytologist
New Phytologist 生物-植物科学
自引率
5.30%
发文量
728
期刊介绍: New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.
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