Jeong-Byoung Chae, Seung-Uk Shin, Serim Kim, Hansong Chae, Won Gyeong Kim, Joon-Seok Chae, Hyuk Song, Jung-Won Kang
{"title":"在大韩民国鉴定出一种新的牛皮卡病毒(Boosepivirus)。","authors":"Jeong-Byoung Chae, Seung-Uk Shin, Serim Kim, Hansong Chae, Won Gyeong Kim, Joon-Seok Chae, Hyuk Song, Jung-Won Kang","doi":"10.4142/jvs.24148","DOIUrl":null,"url":null,"abstract":"<p><strong>Importance: </strong>Despite advancements in herd management, feeding, and pharmaceutical interventions, neonatal calf diarrhea (NCD) remains a major global health concern. Bacteria, viruses, and parasites are the major contributors to NCD. Although several pathogens have been identified in the Republic of Korea (ROK), the etiological agents of numerous NCD cases have not been identified.</p><p><strong>Objective: </strong>To identify, for the first time, the prevalence and impact of <i>Boosepivirus</i> (BooV) on calf diarrhea in the ROK.</p><p><strong>Methods: </strong>Here, the unknown cause of calf diarrhea was determined using metagenomics We then explored the prevalence of certain pathogens, including BooV, that cause NCD. Seventy diarrheal fecal samples from Hanwoo (<i>Bos taurus coreanae</i>) calves were analyzed using reverse transcriptase and quantitative real-time polymerase chain reaction for pathogen detection and BooV isolate sequencing.</p><p><strong>Results: </strong>The complete genome of BooV was detected from unknown causes of calf diarrhea. And also, BooV was the most frequently detected pathogen (35.7%) among 8 pathogens in 70 diarrheic feces from Hanwoo calves. Co-infection analyses indicated that most BooV-positive samples were solely infected with BooV, indicating its significance in NCD in the ROK. All isolates were classified as BooV B in phylogenetic analysis.</p><p><strong>Conclusions and relevance: </strong>This is the first study to determine the prevalence and molecular characteristics of BooV in calf diarrhea in the ROK, highlighting the potential importance of BooV as a causative agent of calf diarrhea and highlighting the need for further research on its epidemiology and pathogenicity.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":"e59"},"PeriodicalIF":1.5000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11450388/pdf/","citationCount":"0","resultStr":"{\"title\":\"Identification of a new bovine picornavirus (<i>Boosepivirus</i>) in the Republic of Korea.\",\"authors\":\"Jeong-Byoung Chae, Seung-Uk Shin, Serim Kim, Hansong Chae, Won Gyeong Kim, Joon-Seok Chae, Hyuk Song, Jung-Won Kang\",\"doi\":\"10.4142/jvs.24148\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Importance: </strong>Despite advancements in herd management, feeding, and pharmaceutical interventions, neonatal calf diarrhea (NCD) remains a major global health concern. Bacteria, viruses, and parasites are the major contributors to NCD. Although several pathogens have been identified in the Republic of Korea (ROK), the etiological agents of numerous NCD cases have not been identified.</p><p><strong>Objective: </strong>To identify, for the first time, the prevalence and impact of <i>Boosepivirus</i> (BooV) on calf diarrhea in the ROK.</p><p><strong>Methods: </strong>Here, the unknown cause of calf diarrhea was determined using metagenomics We then explored the prevalence of certain pathogens, including BooV, that cause NCD. Seventy diarrheal fecal samples from Hanwoo (<i>Bos taurus coreanae</i>) calves were analyzed using reverse transcriptase and quantitative real-time polymerase chain reaction for pathogen detection and BooV isolate sequencing.</p><p><strong>Results: </strong>The complete genome of BooV was detected from unknown causes of calf diarrhea. And also, BooV was the most frequently detected pathogen (35.7%) among 8 pathogens in 70 diarrheic feces from Hanwoo calves. Co-infection analyses indicated that most BooV-positive samples were solely infected with BooV, indicating its significance in NCD in the ROK. All isolates were classified as BooV B in phylogenetic analysis.</p><p><strong>Conclusions and relevance: </strong>This is the first study to determine the prevalence and molecular characteristics of BooV in calf diarrhea in the ROK, highlighting the potential importance of BooV as a causative agent of calf diarrhea and highlighting the need for further research on its epidemiology and pathogenicity.</p>\",\"PeriodicalId\":17557,\"journal\":{\"name\":\"Journal of Veterinary Science\",\"volume\":\" \",\"pages\":\"e59\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2024-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11450388/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Veterinary Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.4142/jvs.24148\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/8/6 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.4142/jvs.24148","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/6 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Identification of a new bovine picornavirus (Boosepivirus) in the Republic of Korea.
Importance: Despite advancements in herd management, feeding, and pharmaceutical interventions, neonatal calf diarrhea (NCD) remains a major global health concern. Bacteria, viruses, and parasites are the major contributors to NCD. Although several pathogens have been identified in the Republic of Korea (ROK), the etiological agents of numerous NCD cases have not been identified.
Objective: To identify, for the first time, the prevalence and impact of Boosepivirus (BooV) on calf diarrhea in the ROK.
Methods: Here, the unknown cause of calf diarrhea was determined using metagenomics We then explored the prevalence of certain pathogens, including BooV, that cause NCD. Seventy diarrheal fecal samples from Hanwoo (Bos taurus coreanae) calves were analyzed using reverse transcriptase and quantitative real-time polymerase chain reaction for pathogen detection and BooV isolate sequencing.
Results: The complete genome of BooV was detected from unknown causes of calf diarrhea. And also, BooV was the most frequently detected pathogen (35.7%) among 8 pathogens in 70 diarrheic feces from Hanwoo calves. Co-infection analyses indicated that most BooV-positive samples were solely infected with BooV, indicating its significance in NCD in the ROK. All isolates were classified as BooV B in phylogenetic analysis.
Conclusions and relevance: This is the first study to determine the prevalence and molecular characteristics of BooV in calf diarrhea in the ROK, highlighting the potential importance of BooV as a causative agent of calf diarrhea and highlighting the need for further research on its epidemiology and pathogenicity.
期刊介绍:
The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.