{"title":"单一宿主物种中的三种雌化沃尔巴奇菌株:比较基因组学为确定性别逆转因子铺平了道路。","authors":"Pierre Grève, Bouziane Moumen, Didier Bouchon","doi":"10.3389/fmicb.2024.1416057","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Endosymbiotic bacteria in the genus <i>Wolbachia</i> have evolved numerous strategies for manipulating host reproduction in order to promote their own transmission. This includes the feminization of males into functional females, a well-studied phenotype in the isopod <i>Armadillidium vulgare</i>. Despite an early description of this phenotype in isopods and the development of an evolutionary model of host sex determination in the presence of <i>Wolbachia</i>, the underlying genetic mechanisms remain elusive.</p><p><strong>Methods: </strong>Here we present the first complete genomes of the three feminizing <i>Wolbachia</i> (<i>w</i>VulC, <i>w</i>VulP, and <i>w</i>VulM) known to date in <i>A. vulgare</i>. These genomes, belonging to <i>Wolbachia</i> B supergroup, contain a large number of mobile elements such as WO prophages with eukaryotic association modules. Taking advantage of these data and those of another <i>Wolbachia</i>-derived feminizing factor integrated into the host genome (<i>f</i> element), we used a comparative genomics approach to identify putative feminizing factors.</p><p><strong>Results: </strong>This strategy has enabled us to identify three prophage-associated genes secreted by the Type IV Secretion System: one ankyrin repeat domain-containing protein, one helix-turn-helix transcriptional regulator and one hypothetical protein. In addition, a latrotoxin-related protein, associated with phage relic genes, was shared by all three genomes and the <i>f</i> element.</p><p><strong>Conclusion: </strong>These putative feminization-inducing proteins shared canonical interaction features with eukaryotic proteins. 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引用次数: 0
摘要
导言:沃尔巴奇属的内共生细菌已经进化出许多操纵宿主繁殖的策略,以促进自身的传播。其中包括将雄性雌化为功能性雌性,这是在等足类动物犰狳(Armadillidium vulgare)中得到充分研究的一种表型。方法:在此,我们首次展示了迄今已知的三种雌性化沃尔巴克氏体(wVulC、wVulP 和 wVulM)的完整基因组。这些基因组属于 Wolbachia B 超群,包含大量移动元素,如带有真核关联模块的 WO 亲体。利用这些数据以及另一种整合到宿主基因组中的沃尔巴克氏体衍生的雌性化因子(f 元素)的数据,我们采用了比较基因组学方法来鉴定推定的雌性化因子:结果:这一策略使我们确定了由 IV 型分泌系统分泌的三个雌激素相关基因:一个含烯丙基蛋白重复结构域的蛋白、一个螺旋-翻转-螺旋转录调节因子和一个假定蛋白。此外,与噬菌体遗物基因相关的一种 latrotoxin 相关蛋白为所有三个基因组和 f 元素所共有:结论:这些假定的雌性化诱导蛋白与真核生物蛋白共享典型的相互作用特征。这些结果为进一步研究潜在的功能相互作用铺平了道路。
Three feminizing Wolbachia strains in a single host species: comparative genomics paves the way for identifying sex reversal factors.
Introduction: Endosymbiotic bacteria in the genus Wolbachia have evolved numerous strategies for manipulating host reproduction in order to promote their own transmission. This includes the feminization of males into functional females, a well-studied phenotype in the isopod Armadillidium vulgare. Despite an early description of this phenotype in isopods and the development of an evolutionary model of host sex determination in the presence of Wolbachia, the underlying genetic mechanisms remain elusive.
Methods: Here we present the first complete genomes of the three feminizing Wolbachia (wVulC, wVulP, and wVulM) known to date in A. vulgare. These genomes, belonging to Wolbachia B supergroup, contain a large number of mobile elements such as WO prophages with eukaryotic association modules. Taking advantage of these data and those of another Wolbachia-derived feminizing factor integrated into the host genome (f element), we used a comparative genomics approach to identify putative feminizing factors.
Results: This strategy has enabled us to identify three prophage-associated genes secreted by the Type IV Secretion System: one ankyrin repeat domain-containing protein, one helix-turn-helix transcriptional regulator and one hypothetical protein. In addition, a latrotoxin-related protein, associated with phage relic genes, was shared by all three genomes and the f element.
Conclusion: These putative feminization-inducing proteins shared canonical interaction features with eukaryotic proteins. These results pave the way for further research into the underlying functional interactions.
期刊介绍:
Frontiers in Microbiology is a leading journal in its field, publishing rigorously peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.