蛋白质组学和数字减影血管造影方法揭示了 CDH18 是治疗 moyamoya 病的潜在靶点。

IF 5.7 2区生物学 Q1 BIOLOGY

Biology Direct Pub Date : 2024-09-05 DOI:10.1186/s13062-024-00522-w

Dong Guo, Yang Dong, Hongbin Li, Hongwei Li, Bo Yang

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引用次数: 0

摘要

研究人员对以大脑基底动脉阻塞为特征的莫亚莫亚病进行了蛋白质差异表达研究，以阐明其发病机制。根据术后血管造影术将 10 名患者分为预后良好组和预后不良组，对这些患者的脑脊液进行的蛋白质组学分析发现了 46 种不同表达的蛋白质。值得注意的是，在预后良好组中，粘连蛋白 18（CDH18）的上调最为显著。此外，还通过 qRT-PCR 和 Western blot 检测了粘连蛋白 18（CDH18）和表型转化相关蛋白的表达。CDH18对血管平滑肌细胞的影响通过CCK-8、EdU、transwell和伤口愈合实验进行了检测。在血管平滑肌细胞（VSMCs）中过表达 CDH18 可抑制其增殖、迁移和表型转化。这些发现表明 CDH18 是治疗 moyamoya 病的潜在靶点。

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Proteomics and digital subtraction angiography approaches reveal CDH18 as a potential target for therapy of moyamoya disease.

Moyamoya disease, characterized by basal cerebral artery obstruction, was studied for differential protein expression to elucidate its pathogenesis. Proteomic analysis of cerebrospinal fluid from 10 patients, categorized by postoperative angiography into good and poor prognosis groups, revealed 46 differentially expressed proteins. Notably, cadherin 18 (CDH18) was the most significantly upregulated in the good prognosis group. In addition, the expression of cadherin 18 (CDH18) and phenotypic transformation-related proteins were measured by qRT-PCR and western blot. The effects of CDH18 in vascular smooth muscle cells were detected by CCK-8, EdU, transwell and wound healing assays. The overexpression of CDH18 in vascular smooth muscle cells (VSMCs) was found to inhibit proliferation, migration, and phenotypic transformation. These findings suggest CDH18 as a potential therapeutic target in moyamoya disease.

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来源期刊

Biology Direct 生物-生物学

CiteScore

6.40

自引率

10.90%

发文量

审稿时长

7 months

期刊介绍： Biology Direct serves the life science research community as an open access, peer-reviewed online journal, providing authors and readers with an alternative to the traditional model of peer review. Biology Direct considers original research articles, hypotheses, comments, discovery notes and reviews in subject areas currently identified as those most conducive to the open review approach, primarily those with a significant non-experimental component.