活细胞内单线态氧的产生:磷光衰减寿命、定位和光动力作用结果之间的相关性。

IF 2.7 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Photochemical & Photobiological Sciences Pub Date : 2024-09-01 Epub Date: 2024-09-05 DOI:10.1007/s43630-024-00620-8
Grzegorz Szewczyk, Krystian Mokrzyński, Tadeusz Sarna
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引用次数: 0

摘要

光动力疗法(PDT)是利用光敏剂(PS)在光激活时产生的活性氧(ROS)治疗局部病变和感染的一种很有前景的替代疗法。单线态氧(1O2)是造成光动力损伤的主要 ROS。然而,光敏剂在生物系统中的有效性可能与单线态氧在均匀溶液中的生成效率并不相关。本研究利用脂质体和 ARPE-19 细胞模型研究了各种细胞微环境中单线态氧的生成及其衰减。研究选择了玫瑰红(RB)、亚甲基蓝(MB)和原卟啉 IX(PpIX)作为 PS。所选光敏剂在不同细胞区室产生的单线态氧的寿命各不相同,表明单线态氧的淬灭速率不同。位于细胞膜附近的 RB 显示出最高的光毒性和脂质/蛋白质过氧化作用,其次是 PpIX,而 MB 在类似条件下显示出最小的细胞毒性。单线态氧衰变寿命提供了对 PS 定位和潜在光毒性的深入了解,突出了脂质微环境在光动力疗法疗效中的重要性,为体内应用提供了有用的筛选方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Generation of singlet oxygen inside living cells: correlation between phosphorescence decay lifetime, localization and outcome of photodynamic action.

Generation of singlet oxygen inside living cells: correlation between phosphorescence decay lifetime, localization and outcome of photodynamic action.

Photodynamic therapy (PDT) is a promising alternative treatment for localized lesions and infections, utilizing reactive oxygen species (ROS) generated by photosensitizers (PS) upon light activation. Singlet oxygen (1O2) is a key ROS responsible for photodynamic damage. However, the effectiveness of PS in biological systems may not correlate with the efficiency of singlet oxygen generation in homogeneous solutions. This study investigated singlet oxygen generation and its decay in various cellular microenvironments using liposome and ARPE-19 cell models. Rose Bengal (RB), methylene blue (MB), and protoporphyrin IX (PpIX) were employed as selected PS. Lifetimes of singlet oxygen generated by the selected photosensitizers in different cellular compartments varied, indicating different quenching rates with singlet oxygen. RB, located near cell membranes, exhibited the highest phototoxicity and lipid/protein peroxidation, followed by PpIX, while MB showed minimal cytotoxicity in similar conditions. Singlet oxygen decay lifetimes provide insights into PS localization and potential phototoxicity, highlighting the importance of the lipid microenvironment in PDT efficacy, providing useful screening method prior to in vivo applications.

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来源期刊
Photochemical & Photobiological Sciences
Photochemical & Photobiological Sciences 生物-生化与分子生物学
CiteScore
5.60
自引率
6.50%
发文量
201
审稿时长
2.3 months
期刊介绍: A society-owned journal publishing high quality research on all aspects of photochemistry and photobiology.
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