拟南芥细胞表面免疫受体 LORE 跨家族转移到番茄后,能感知 3- 羟基化脂肪酸并增强抗病性。

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES
Sabine Eschrig, Parvinderdeep S Kahlon, Carlos Agius, Andrea Holzer, Ralph Hückelhoven, Claus Schwechheimer, Stefanie Ranf
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引用次数: 0

摘要

植物病原体极易造成减产并威胁粮食安全。科技进步提高了我们对宿主与病原体相互作用的分子过程的认识,这为超越传统育种限制的作物病害管理新策略铺平了道路。免疫受体基因的跨家族转移就是这样一种策略,它利用常见的植物免疫信号通路来提高作物的抗病性。质膜上驻留的模式识别受体(PRR)对微生物或宿主损伤相关分子模式(MAMPs/DAMPs)的感应激活了模式触发免疫(PTI),并限制了多种病原体在宿主植物中的传播。在模式植物拟南芥中,S-domain 受体样激酶 LIPOOLIGOSACCHARIDE-SPECIFICED RUCED ELICITATION(ATLORE,SD1-29)具有 PRR 的功能,它能感知中链长度的 3-hydroxylated fatty acids(mc-3-OH-FAs),如 3-OH-C10:0 和微生物来源的 3-hydroxyalkanoates (HAAs),从而激活 PTI。本研究表明,在茄科作物番茄(Solanum lycopersicum)中异位表达十字花科特异性 PRR AtLORE 可获得 3-OH-C10:0 免疫感应,而不会改变植物的发育。转基因番茄 AtLORE 对番茄假单胞菌 DC3000 和茄属交替孢霉 NL03003 的抗性增强。在感染前向土壤中施用 3-OH-C10:0 可诱导对卵菌病原体 Phytophthora infestans Pi100 的抗性,并进一步增强对 A. solani NL03003 的抗性。我们的研究提出了 AtLORE 转基因作物植物和 mc-3-OH-FAs 作为抗性诱导生物刺激剂在病害防治中的潜在应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cross-family transfer of the Arabidopsis cell-surface immune receptor LORE to tomato confers sensing of 3-hydroxylated fatty acids and enhanced disease resistance.

Plant pathogens pose a high risk of yield losses and threaten food security. Technological and scientific advances have improved our understanding of the molecular processes underlying host-pathogen interactions, which paves the way for new strategies in crop disease management beyond the limits of conventional breeding. Cross-family transfer of immune receptor genes is one such strategy that takes advantage of common plant immune signalling pathways to improve disease resistance in crops. Sensing of microbe- or host damage-associated molecular patterns (MAMPs/DAMPs) by plasma membrane-resident pattern recognition receptors (PRR) activates pattern-triggered immunity (PTI) and restricts the spread of a broad spectrum of pathogens in the host plant. In the model plant Arabidopsis thaliana, the S-domain receptor-like kinase LIPOOLIGOSACCHARIDE-SPECIFIC REDUCED ELICITATION (AtLORE, SD1-29) functions as a PRR, which senses medium-chain-length 3-hydroxylated fatty acids (mc-3-OH-FAs), such as 3-OH-C10:0, and 3-hydroxyalkanoates (HAAs) of microbial origin to activate PTI. In this study, we show that ectopic expression of the Brassicaceae-specific PRR AtLORE in the solanaceous crop species Solanum lycopersicum leads to the gain of 3-OH-C10:0 immune sensing without altering plant development. AtLORE-transgenic tomato shows enhanced resistance against Pseudomonas syringae pv. tomato DC3000 and Alternaria solani NL03003. Applying 3-OH-C10:0 to the soil before infection induces resistance against the oomycete pathogen Phytophthora infestans Pi100 and further enhances resistance to A. solani NL03003. Our study proposes a potential application of AtLORE-transgenic crop plants and mc-3-OH-FAs as resistance-inducing biostimulants in disease management.

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来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
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