Fernanda Danielle Melo, Heloíse Peterle, Ricardo Antonio Pilegi Sfaciotte, Mateus Felipe Schneider, Leandro Parussolo, Sheila Rezler Wosiacki, Ubirajara Maciel da Costa, Sandra Maria Ferraz
{"title":"从生乳和手工奶酪中分离的葡萄球菌的抗菌谱表型和基因型从生乳和手工奶酪中分离的葡萄球菌的抗菌谱。","authors":"Fernanda Danielle Melo, Heloíse Peterle, Ricardo Antonio Pilegi Sfaciotte, Mateus Felipe Schneider, Leandro Parussolo, Sheila Rezler Wosiacki, Ubirajara Maciel da Costa, Sandra Maria Ferraz","doi":"10.1089/fpd.2024.0037","DOIUrl":null,"url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> and a few species of coagulase negative are frequently associated with food poisoning. Raw milk and dairy products are among the foods usually associated with outbreaks due to staphylococcal intoxication. This study aimed to determine phenotypic and genotypic antimicrobial resistance profiles to beta-lactam drugs in <i>Staphylococcus</i> coagulase positive (CoPS) and negative (CoNS) isolates. A total of 58 CoPS and 45 CoNS isolates recovered from raw milk and artisanal cheese from Santa Catarina were analyzed. All isolates (<i>n</i> = 103) were subjected to antimicrobial susceptibility testing. High levels of resistance to penicillin (41% of CoPS and 31% of CoNS), amoxicillin (40% CoPS), ampicillin (36% CoPS), and sulfamethoxazole-trimethoprim (35% CoNS) were observed. Twenty six percent of the isolates (18 CoPS and 9 CoNS) exhibited multiresistance profile; which means, they were resistant to at least three different classes of the antimicrobial drugs. Detection of resistance genes (<i>mec</i>A, <i>mec</i>C, and <i>bla</i>Z) was performed using multiplex polymerase chain reaction. Twelve isolates (9 CoPS and 3 CoNS) were positive for <i>mec</i>A, whereas 10 strains (4 CoPS and 6 CoNS) were positive for <i>bla</i>Z. The detection of resistant and multidrug resistant isolates emphasizes the necessity to develop strategies to better comply with good manufacturing practices and health care guidelines.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phenotypic and Genotypic of Antimicrobial Resistance Profile of <i>Staphylococcu</i>s Spp. Isolated from Raw Milk and Artisanal Cheese.\",\"authors\":\"Fernanda Danielle Melo, Heloíse Peterle, Ricardo Antonio Pilegi Sfaciotte, Mateus Felipe Schneider, Leandro Parussolo, Sheila Rezler Wosiacki, Ubirajara Maciel da Costa, Sandra Maria Ferraz\",\"doi\":\"10.1089/fpd.2024.0037\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Staphylococcus aureus</i> and a few species of coagulase negative are frequently associated with food poisoning. Raw milk and dairy products are among the foods usually associated with outbreaks due to staphylococcal intoxication. This study aimed to determine phenotypic and genotypic antimicrobial resistance profiles to beta-lactam drugs in <i>Staphylococcus</i> coagulase positive (CoPS) and negative (CoNS) isolates. A total of 58 CoPS and 45 CoNS isolates recovered from raw milk and artisanal cheese from Santa Catarina were analyzed. All isolates (<i>n</i> = 103) were subjected to antimicrobial susceptibility testing. High levels of resistance to penicillin (41% of CoPS and 31% of CoNS), amoxicillin (40% CoPS), ampicillin (36% CoPS), and sulfamethoxazole-trimethoprim (35% CoNS) were observed. Twenty six percent of the isolates (18 CoPS and 9 CoNS) exhibited multiresistance profile; which means, they were resistant to at least three different classes of the antimicrobial drugs. Detection of resistance genes (<i>mec</i>A, <i>mec</i>C, and <i>bla</i>Z) was performed using multiplex polymerase chain reaction. Twelve isolates (9 CoPS and 3 CoNS) were positive for <i>mec</i>A, whereas 10 strains (4 CoPS and 6 CoNS) were positive for <i>bla</i>Z. The detection of resistant and multidrug resistant isolates emphasizes the necessity to develop strategies to better comply with good manufacturing practices and health care guidelines.</p>\",\"PeriodicalId\":12333,\"journal\":{\"name\":\"Foodborne pathogens and disease\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2024-09-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Foodborne pathogens and disease\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1089/fpd.2024.0037\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Foodborne pathogens and disease","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1089/fpd.2024.0037","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
Phenotypic and Genotypic of Antimicrobial Resistance Profile of Staphylococcus Spp. Isolated from Raw Milk and Artisanal Cheese.
Staphylococcus aureus and a few species of coagulase negative are frequently associated with food poisoning. Raw milk and dairy products are among the foods usually associated with outbreaks due to staphylococcal intoxication. This study aimed to determine phenotypic and genotypic antimicrobial resistance profiles to beta-lactam drugs in Staphylococcus coagulase positive (CoPS) and negative (CoNS) isolates. A total of 58 CoPS and 45 CoNS isolates recovered from raw milk and artisanal cheese from Santa Catarina were analyzed. All isolates (n = 103) were subjected to antimicrobial susceptibility testing. High levels of resistance to penicillin (41% of CoPS and 31% of CoNS), amoxicillin (40% CoPS), ampicillin (36% CoPS), and sulfamethoxazole-trimethoprim (35% CoNS) were observed. Twenty six percent of the isolates (18 CoPS and 9 CoNS) exhibited multiresistance profile; which means, they were resistant to at least three different classes of the antimicrobial drugs. Detection of resistance genes (mecA, mecC, and blaZ) was performed using multiplex polymerase chain reaction. Twelve isolates (9 CoPS and 3 CoNS) were positive for mecA, whereas 10 strains (4 CoPS and 6 CoNS) were positive for blaZ. The detection of resistant and multidrug resistant isolates emphasizes the necessity to develop strategies to better comply with good manufacturing practices and health care guidelines.
期刊介绍:
Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide.
Foodborne Pathogens and Disease coverage includes:
Agroterrorism
Safety of organically grown and genetically modified foods
Emerging pathogens
Emergence of drug resistance
Methods and technology for rapid and accurate detection
Strategies to destroy or control foodborne pathogens
Novel strategies for the prevention and control of plant and animal diseases that impact food safety
Biosecurity issues and the implications of new regulatory guidelines
Impact of changing lifestyles and consumer demands on food safety.