对头颈部癌症化疗患者外周血中不同的淋巴细胞和骨髓细胞群进行全面和纵向评估。

IF 4.6 2区 医学 Q2 IMMUNOLOGY
Jens von der Grün, Martina Broglie, Matthias Guckenberger, Panagiotis Balermpas
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引用次数: 0

摘要

背景:免疫疗法为复发和转移性头颈癌患者带来了明显的生存益处。这些改善无法在接受治愈性放化疗(CRT)的患者身上重现,最佳的放射免疫疗法(RIT)概念仍有待设计。探索和分析这些患者治疗前的免疫状态以及治疗过程中发生的变化,对于合理设计未来的联合疗法至关重要:方法:在治疗前、治疗第一周后和治疗结束三个月后,从25名接受治疗性意向(C)-RT治疗的头颈部癌症患者中采集血液样本。外周血单核细胞(PBMC)或所有有核血细胞被分离出来,并通过流式细胞术进行分析:结果:与健康人相比,基线期患者的单核细胞和淋巴细胞数量减少。虽然患者体内 CD8+ T 细胞的总体频率降低了,但记忆亚群的比例却增加了。放疗(RT)治疗使CD8+效应记忆T细胞进一步增加。在骨髓细胞群中,RT治疗后肿瘤促进亚群的数量减少,而有利于炎症细胞:本研究前瞻性地展示了头颈部癌症根治性(C)RT期间淋巴细胞和髓系细胞群组成的复杂相互作用和明显纵向变化。在更大的群体中进一步验证这种方法,可在未来提供更好的治疗指导并有针对性地纳入免疫疗法(IT)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A comprehensive and longitudinal evaluation of the different populations of lymphoid and myeloid cells in the peripheral blood of patients treated with chemoradiotherapy for head and neck cancer.

A comprehensive and longitudinal evaluation of the different populations of lymphoid and myeloid cells in the peripheral blood of patients treated with chemoradiotherapy for head and neck cancer.

Background: Immunotherapy provided significant survival benefits for recurrent and metastatic patients with head and neck cancer. These improvements could not be reproduced in patients treated with curative-intent chemoradiotherapy (CRT) and the optimal radio-immunotherapy (RIT) concepts have yet to be designed. Exploration and analysis of the pre-therapeutic immune status of these patients and the changes occurring during the treatment course could be crucial in rationally designing future combined treatments.

Methods: Blood samples were collected from a cohort of 25 head and neck cancer patients treated with curative-intended (C)-RT prior to therapy, after the first week of treatment, and three months after treatment completion. Peripheral blood mononuclear cells (PBMCs) or all nucleated blood cells were isolated and analyzed via flow cytometry.

Results: At baseline, patients showed reduced monocyte and lymphocyte counts compared to healthy individuals. Although overall CD8+ T-cell frequencies were reduced, the proportion of memory subsets were increased in patients. Radiotherapy (RT) treatment led to a further increase in CD8+ effector memory T-cells. Among myeloid populations, tumor-promoting subsets became less abundant after RT, in favor of pro-inflammatory cells.

Conclusion: The present study prospectively demonstrated a complex interplay and distinct longitudinal changes in the composition of lymphocytic and myeloid populations during curative (C)-RT of head and neck cancer. Further validation of this method in a larger cohort could allow for better treatment guidance and tailored incorporation of immunotherapies (IT) in the future.

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来源期刊
CiteScore
10.50
自引率
1.70%
发文量
207
审稿时长
1 months
期刊介绍: Cancer Immunology, Immunotherapy has the basic aim of keeping readers informed of the latest research results in the fields of oncology and immunology. As knowledge expands, the scope of the journal has broadened to include more of the progress being made in the areas of biology concerned with biological response modifiers. This helps keep readers up to date on the latest advances in our understanding of tumor-host interactions. The journal publishes short editorials including "position papers," general reviews, original articles, and short communications, providing a forum for the most current experimental and clinical advances in tumor immunology.
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