Mehak Kaur, Rahul C Mishra, Vaibhavi Lahane, Anita Kumari, Akhilesh K Yadav, Monika Garg, Colin J Barrow, Mayurika Goel
{"title":"从柠檬香茅(Cymbopogon citratus)中分离出的内生真菌 Curvularia Lunata 的化学特征和生物活性。","authors":"Mehak Kaur, Rahul C Mishra, Vaibhavi Lahane, Anita Kumari, Akhilesh K Yadav, Monika Garg, Colin J Barrow, Mayurika Goel","doi":"10.1007/s42770-024-01503-x","DOIUrl":null,"url":null,"abstract":"<p><p>Exploration of medicinal plants for bioactive-producing endophytic fungi is a relatively unmapped source of pharmaceutically important compounds. In this study, the endophytic fungus Curvularia lunata AREF029 isolated from the medicinal plant Cymbopogon citratus (known as lemongrass) was assessed for its biological activity. The methanolic extract of AREF029 had minimum inhibition concentration (MIC) ranging from 38 to 174 µg/ml against phytopathogenic fungi Alteranria solani, Fusarium oxysporum and Rhizoctonia solani. Furthermore, the AREF029 methanolic extract displayed a broad-spectrum MIC of 25 µg/ml in the case of Staphylococcus aureus, Salmonella typhimurium and MRSA (methicillin-resistant S. aureus). In vitro cytotoxicity analysis with murine macrophage cell line RAW 264.7 determined 56% nitric oxide inhibition activity at 200 µg/ml concentration of the extract and more than 99% cell viability. Gas chromatography-mass spectrometry (GC-MS) and Liquid chromatography-high resolution mass spectrometry (LC-HRMS) analyses showed the presence of methoxy-5-methyl-4-oxo-2,5-hexadienoic acid (penicillic acid), phthalic acid, bis (7-methyloctyl) ester, 8-hydroxyquinoline, tetroquinone, curvulamine, Curvuleremophilane B/D, Chromonilinc acid A/C and other putative bioactive compounds in the extract. The current investigation supports the significance of the endophytic fungus C. lunata as a source of potent antibacterial, antifungal and anti-inflammatory compounds.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Chemical characterization and biological activity of Curvularia Lunata, an endophytic fungus isolated from lemongrass (Cymbopogon citratus).\",\"authors\":\"Mehak Kaur, Rahul C Mishra, Vaibhavi Lahane, Anita Kumari, Akhilesh K Yadav, Monika Garg, Colin J Barrow, Mayurika Goel\",\"doi\":\"10.1007/s42770-024-01503-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Exploration of medicinal plants for bioactive-producing endophytic fungi is a relatively unmapped source of pharmaceutically important compounds. In this study, the endophytic fungus Curvularia lunata AREF029 isolated from the medicinal plant Cymbopogon citratus (known as lemongrass) was assessed for its biological activity. The methanolic extract of AREF029 had minimum inhibition concentration (MIC) ranging from 38 to 174 µg/ml against phytopathogenic fungi Alteranria solani, Fusarium oxysporum and Rhizoctonia solani. Furthermore, the AREF029 methanolic extract displayed a broad-spectrum MIC of 25 µg/ml in the case of Staphylococcus aureus, Salmonella typhimurium and MRSA (methicillin-resistant S. aureus). In vitro cytotoxicity analysis with murine macrophage cell line RAW 264.7 determined 56% nitric oxide inhibition activity at 200 µg/ml concentration of the extract and more than 99% cell viability. Gas chromatography-mass spectrometry (GC-MS) and Liquid chromatography-high resolution mass spectrometry (LC-HRMS) analyses showed the presence of methoxy-5-methyl-4-oxo-2,5-hexadienoic acid (penicillic acid), phthalic acid, bis (7-methyloctyl) ester, 8-hydroxyquinoline, tetroquinone, curvulamine, Curvuleremophilane B/D, Chromonilinc acid A/C and other putative bioactive compounds in the extract. The current investigation supports the significance of the endophytic fungus C. lunata as a source of potent antibacterial, antifungal and anti-inflammatory compounds.</p>\",\"PeriodicalId\":9090,\"journal\":{\"name\":\"Brazilian Journal of Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-09-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Brazilian Journal of Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s42770-024-01503-x\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brazilian Journal of Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s42770-024-01503-x","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Chemical characterization and biological activity of Curvularia Lunata, an endophytic fungus isolated from lemongrass (Cymbopogon citratus).
Exploration of medicinal plants for bioactive-producing endophytic fungi is a relatively unmapped source of pharmaceutically important compounds. In this study, the endophytic fungus Curvularia lunata AREF029 isolated from the medicinal plant Cymbopogon citratus (known as lemongrass) was assessed for its biological activity. The methanolic extract of AREF029 had minimum inhibition concentration (MIC) ranging from 38 to 174 µg/ml against phytopathogenic fungi Alteranria solani, Fusarium oxysporum and Rhizoctonia solani. Furthermore, the AREF029 methanolic extract displayed a broad-spectrum MIC of 25 µg/ml in the case of Staphylococcus aureus, Salmonella typhimurium and MRSA (methicillin-resistant S. aureus). In vitro cytotoxicity analysis with murine macrophage cell line RAW 264.7 determined 56% nitric oxide inhibition activity at 200 µg/ml concentration of the extract and more than 99% cell viability. Gas chromatography-mass spectrometry (GC-MS) and Liquid chromatography-high resolution mass spectrometry (LC-HRMS) analyses showed the presence of methoxy-5-methyl-4-oxo-2,5-hexadienoic acid (penicillic acid), phthalic acid, bis (7-methyloctyl) ester, 8-hydroxyquinoline, tetroquinone, curvulamine, Curvuleremophilane B/D, Chromonilinc acid A/C and other putative bioactive compounds in the extract. The current investigation supports the significance of the endophytic fungus C. lunata as a source of potent antibacterial, antifungal and anti-inflammatory compounds.
期刊介绍:
The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology.
The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors.
The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.