从柠檬香茅(Cymbopogon citratus)中分离出的内生真菌 Curvularia Lunata 的化学特征和生物活性。

IF 2.1 4区 生物学 Q3 MICROBIOLOGY
Mehak Kaur, Rahul C Mishra, Vaibhavi Lahane, Anita Kumari, Akhilesh K Yadav, Monika Garg, Colin J Barrow, Mayurika Goel
{"title":"从柠檬香茅(Cymbopogon citratus)中分离出的内生真菌 Curvularia Lunata 的化学特征和生物活性。","authors":"Mehak Kaur, Rahul C Mishra, Vaibhavi Lahane, Anita Kumari, Akhilesh K Yadav, Monika Garg, Colin J Barrow, Mayurika Goel","doi":"10.1007/s42770-024-01503-x","DOIUrl":null,"url":null,"abstract":"<p><p>Exploration of medicinal plants for bioactive-producing endophytic fungi is a relatively unmapped source of pharmaceutically important compounds. In this study, the endophytic fungus Curvularia lunata AREF029 isolated from the medicinal plant Cymbopogon citratus (known as lemongrass) was assessed for its biological activity. The methanolic extract of AREF029 had minimum inhibition concentration (MIC) ranging from 38 to 174 µg/ml against phytopathogenic fungi Alteranria solani, Fusarium oxysporum and Rhizoctonia solani. Furthermore, the AREF029 methanolic extract displayed a broad-spectrum MIC of 25 µg/ml in the case of Staphylococcus aureus, Salmonella typhimurium and MRSA (methicillin-resistant S. aureus). In vitro cytotoxicity analysis with murine macrophage cell line RAW 264.7 determined 56% nitric oxide inhibition activity at 200 µg/ml concentration of the extract and more than 99% cell viability. Gas chromatography-mass spectrometry (GC-MS) and Liquid chromatography-high resolution mass spectrometry (LC-HRMS) analyses showed the presence of methoxy-5-methyl-4-oxo-2,5-hexadienoic acid (penicillic acid), phthalic acid, bis (7-methyloctyl) ester, 8-hydroxyquinoline, tetroquinone, curvulamine, Curvuleremophilane B/D, Chromonilinc acid A/C and other putative bioactive compounds in the extract. The current investigation supports the significance of the endophytic fungus C. lunata as a source of potent antibacterial, antifungal and anti-inflammatory compounds.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Chemical characterization and biological activity of Curvularia Lunata, an endophytic fungus isolated from lemongrass (Cymbopogon citratus).\",\"authors\":\"Mehak Kaur, Rahul C Mishra, Vaibhavi Lahane, Anita Kumari, Akhilesh K Yadav, Monika Garg, Colin J Barrow, Mayurika Goel\",\"doi\":\"10.1007/s42770-024-01503-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Exploration of medicinal plants for bioactive-producing endophytic fungi is a relatively unmapped source of pharmaceutically important compounds. In this study, the endophytic fungus Curvularia lunata AREF029 isolated from the medicinal plant Cymbopogon citratus (known as lemongrass) was assessed for its biological activity. The methanolic extract of AREF029 had minimum inhibition concentration (MIC) ranging from 38 to 174 µg/ml against phytopathogenic fungi Alteranria solani, Fusarium oxysporum and Rhizoctonia solani. Furthermore, the AREF029 methanolic extract displayed a broad-spectrum MIC of 25 µg/ml in the case of Staphylococcus aureus, Salmonella typhimurium and MRSA (methicillin-resistant S. aureus). In vitro cytotoxicity analysis with murine macrophage cell line RAW 264.7 determined 56% nitric oxide inhibition activity at 200 µg/ml concentration of the extract and more than 99% cell viability. Gas chromatography-mass spectrometry (GC-MS) and Liquid chromatography-high resolution mass spectrometry (LC-HRMS) analyses showed the presence of methoxy-5-methyl-4-oxo-2,5-hexadienoic acid (penicillic acid), phthalic acid, bis (7-methyloctyl) ester, 8-hydroxyquinoline, tetroquinone, curvulamine, Curvuleremophilane B/D, Chromonilinc acid A/C and other putative bioactive compounds in the extract. The current investigation supports the significance of the endophytic fungus C. lunata as a source of potent antibacterial, antifungal and anti-inflammatory compounds.</p>\",\"PeriodicalId\":9090,\"journal\":{\"name\":\"Brazilian Journal of Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-09-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Brazilian Journal of Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s42770-024-01503-x\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brazilian Journal of Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s42770-024-01503-x","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

从药用植物中发掘产生生物活性的内生真菌,是一个相对尚未开发的具有重要药用价值的化合物来源。本研究评估了从药用植物柠檬香茅(Cymbopogon citratus,又称柠檬草)中分离出的内生真菌 Curvularia lunata AREF029 的生物活性。AREF029 的甲醇提取物对植物病原真菌 Alteranria solani、Fusarium oxysporum 和 Rhizoctonia solani 的最小抑菌浓度为 38 至 174 µg/ml。此外,AREF029 甲醇提取物对金黄色葡萄球菌、伤寒沙门氏菌和 MRSA(耐甲氧西林金黄色葡萄球菌)的广谱 MIC 为 25 µg/ml。对小鼠巨噬细胞系 RAW 264.7 进行的体外细胞毒性分析表明,在 200 µg/ml 浓度的提取物中,一氧化氮抑制活性为 56%,细胞存活率超过 99%。气相色谱-质谱(GC-MS)和液相色谱-高分辨质谱(LC-HRMS)分析表明,提取物中含有甲氧基-5-甲基-4-氧代-2,5-己二烯酸(青霉烯酸)、邻苯二甲酸、双邻苯二甲酸、双(7-甲基辛基)酯、8-羟基喹啉、对苯二酚、卷曲霉素、卷曲霉素 B/D、Chromonilinc 酸 A/C 和其他可能的生物活性化合物。目前的研究证实了内生真菌 C. lunata 作为强效抗菌、抗真菌和抗炎化合物来源的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Chemical characterization and biological activity of Curvularia Lunata, an endophytic fungus isolated from lemongrass (Cymbopogon citratus).

Chemical characterization and biological activity of Curvularia Lunata, an endophytic fungus isolated from lemongrass (Cymbopogon citratus).

Exploration of medicinal plants for bioactive-producing endophytic fungi is a relatively unmapped source of pharmaceutically important compounds. In this study, the endophytic fungus Curvularia lunata AREF029 isolated from the medicinal plant Cymbopogon citratus (known as lemongrass) was assessed for its biological activity. The methanolic extract of AREF029 had minimum inhibition concentration (MIC) ranging from 38 to 174 µg/ml against phytopathogenic fungi Alteranria solani, Fusarium oxysporum and Rhizoctonia solani. Furthermore, the AREF029 methanolic extract displayed a broad-spectrum MIC of 25 µg/ml in the case of Staphylococcus aureus, Salmonella typhimurium and MRSA (methicillin-resistant S. aureus). In vitro cytotoxicity analysis with murine macrophage cell line RAW 264.7 determined 56% nitric oxide inhibition activity at 200 µg/ml concentration of the extract and more than 99% cell viability. Gas chromatography-mass spectrometry (GC-MS) and Liquid chromatography-high resolution mass spectrometry (LC-HRMS) analyses showed the presence of methoxy-5-methyl-4-oxo-2,5-hexadienoic acid (penicillic acid), phthalic acid, bis (7-methyloctyl) ester, 8-hydroxyquinoline, tetroquinone, curvulamine, Curvuleremophilane B/D, Chromonilinc acid A/C and other putative bioactive compounds in the extract. The current investigation supports the significance of the endophytic fungus C. lunata as a source of potent antibacterial, antifungal and anti-inflammatory compounds.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信