{"title":"利用固定化花生壳生物炭对染料进行生物修复的机理研究","authors":"","doi":"10.1016/j.envres.2024.119908","DOIUrl":null,"url":null,"abstract":"<div><p>Recalcitrant chemicals in the environment not only present obstacles to living organisms but also contribute to the degradation of natural resources. One contribution to environmental pollution is the discharge of synthetic dyes from the textile sector. This study investigates the combined effect of microbial cells and biochar on eliminating methyl orange (MO) dye. The immobilization of <em>Aeromonas veronii</em> on peanut shell biochar (APSB) was conducted to investigate its efficacy in removing MO dye from water. PSB synthesized by pyrolysis at 300 °C for 120 min showed maximum bacterial immobilization potential. The highest degradation rate of 96.19 % was achieved in APSB within 96 h using MO dye concentration of 100 mg L<sup>−1</sup>, incubation temperature of 37 °C, pH 7, and biocatalyst dosage of 1g L<sup>−1</sup>. In comparison, free cells achieved degradation rates of 72.53 % and 61.56 % for PSB. Moreover, the adsorption process was primarily controlled by PSB, with subsequent dye mineralization by A. veronii, as supported by FTIR and LC-MS studies. Moreover, this innovative approach exhibited the reusability of the biocatalyst, giving 76.23 % removal after fifth cycle, suggesting sustainable alternative in dye remediation and potential option for real-time applications.</p></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":null,"pages":null},"PeriodicalIF":7.7000,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mechanistic studies on bioremediation of dye using Aeromonas veronii immobilized peanut shell biochar\",\"authors\":\"\",\"doi\":\"10.1016/j.envres.2024.119908\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Recalcitrant chemicals in the environment not only present obstacles to living organisms but also contribute to the degradation of natural resources. One contribution to environmental pollution is the discharge of synthetic dyes from the textile sector. This study investigates the combined effect of microbial cells and biochar on eliminating methyl orange (MO) dye. The immobilization of <em>Aeromonas veronii</em> on peanut shell biochar (APSB) was conducted to investigate its efficacy in removing MO dye from water. PSB synthesized by pyrolysis at 300 °C for 120 min showed maximum bacterial immobilization potential. The highest degradation rate of 96.19 % was achieved in APSB within 96 h using MO dye concentration of 100 mg L<sup>−1</sup>, incubation temperature of 37 °C, pH 7, and biocatalyst dosage of 1g L<sup>−1</sup>. In comparison, free cells achieved degradation rates of 72.53 % and 61.56 % for PSB. Moreover, the adsorption process was primarily controlled by PSB, with subsequent dye mineralization by A. veronii, as supported by FTIR and LC-MS studies. Moreover, this innovative approach exhibited the reusability of the biocatalyst, giving 76.23 % removal after fifth cycle, suggesting sustainable alternative in dye remediation and potential option for real-time applications.</p></div>\",\"PeriodicalId\":312,\"journal\":{\"name\":\"Environmental Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":7.7000,\"publicationDate\":\"2024-09-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Research\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0013935124018139\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Research","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0013935124018139","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
环境中的难降解化学品不仅会对生物造成障碍,还会导致自然资源退化。纺织业排放的合成染料是造成环境污染的原因之一。本研究调查了微生物细胞和生物炭对消除甲基橙(MO)染料的综合效果。研究人员在花生壳生物炭(APSB)上固定了 Aeromonas veronii,以考察其去除水中 MO 染料的功效。在 300 °C 下热解 120 分钟合成的 PSB 显示出最大的细菌固定潜力。在 MO 染料浓度为 100 mg L-1、培养温度为 37 °C、pH 值为 7、生物催化剂用量为 1g L-1 的条件下,APSB 在 96 小时内的降解率最高,达到 96.19 %。相比之下,游离细胞的降解率为 72.53%,PSB 的降解率为 61.56%。此外,傅立叶变换红外光谱(FTIR)和液相色谱-质谱(LC-MS)研究表明,吸附过程主要由 PSB 控制,随后由 A. veronii 将染料矿化。此外,这种创新方法还展示了生物催化剂的可重复使用性,第五次循环后的去除率为 76.23%,这表明该方法是一种可持续的染料修复替代方法,具有实时应用的潜力。
Mechanistic studies on bioremediation of dye using Aeromonas veronii immobilized peanut shell biochar
Recalcitrant chemicals in the environment not only present obstacles to living organisms but also contribute to the degradation of natural resources. One contribution to environmental pollution is the discharge of synthetic dyes from the textile sector. This study investigates the combined effect of microbial cells and biochar on eliminating methyl orange (MO) dye. The immobilization of Aeromonas veronii on peanut shell biochar (APSB) was conducted to investigate its efficacy in removing MO dye from water. PSB synthesized by pyrolysis at 300 °C for 120 min showed maximum bacterial immobilization potential. The highest degradation rate of 96.19 % was achieved in APSB within 96 h using MO dye concentration of 100 mg L−1, incubation temperature of 37 °C, pH 7, and biocatalyst dosage of 1g L−1. In comparison, free cells achieved degradation rates of 72.53 % and 61.56 % for PSB. Moreover, the adsorption process was primarily controlled by PSB, with subsequent dye mineralization by A. veronii, as supported by FTIR and LC-MS studies. Moreover, this innovative approach exhibited the reusability of the biocatalyst, giving 76.23 % removal after fifth cycle, suggesting sustainable alternative in dye remediation and potential option for real-time applications.
期刊介绍:
The Environmental Research journal presents a broad range of interdisciplinary research, focused on addressing worldwide environmental concerns and featuring innovative findings. Our publication strives to explore relevant anthropogenic issues across various environmental sectors, showcasing practical applications in real-life settings.