受 MdDREB2A 调控的苹果液泡糖转运体通过促进可溶性糖积累和激活 ABA 信号转导增强抗旱性

IF 8.7 1区 农林科学 Q1 Agricultural and Biological Sciences
Lingcheng Zhu, Chunxia Zhang, Nanxiang Yang, Wenjing Cao, Yanzhen Li, Yunjing Peng, Xiaoyu Wei, Baiquan Ma, Fengwang Ma, Yong-Ling Ruan, Mingjun Li
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引用次数: 0

摘要

可溶性糖不仅是影响果实品质的重要因素,而且还是应对非生物胁迫的渗透调节因子。早期干旱胁迫会促进糖的积累,而特定的糖转运体会控制糖在细胞中的分布。在这里,我们发现苹果小植株在干旱胁迫下会在叶片组织中积累可溶性糖。受胁迫和对照小植株的转录谱分析揭示了几个质膜或液泡膜定位的糖转运体基因的不同表达。其中,之前发现的四个液泡糖转运体(VST)基因(MdERDL6-1、MdERDL6-2、MdTST1和MdTST2)在干旱条件下的表达量较高,表明它们在干旱胁迫下的作用。启动子顺式元件分析、酵母单杂交和双荧光素酶试验证实,干旱诱导的转录因子MdDREB2A可通过与MdERDL6-1/-2和MdTST1/2的启动子区域结合来促进它们的表达。此外,在转基因苹果或拟南芥植株中单独过表达这四种 MdVSTs,可积累更多的可溶性糖和赤霉酸,并增强抗旱性。此外,与对照植株相比,过表达 MdERDL6-1 的苹果植株在干旱条件下还表现出降低水势、促进气孔关闭和清除活性氧的能力。总之,我们的研究结果表明,通过操纵参与液泡糖运输的基因,有可能提高果实的抗旱性和糖分积累。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Apple vacuolar sugar transporters regulated by MdDREB2A enhance drought resistance by promoting accumulation of soluble sugars and activating ABA signaling
Soluble sugars are not only an important contributor to fruit quality, but also serve as the osmotic regulators in response to abiotic stresses. Early drought stress promotes sugar accumulation, while specific sugar transporters govern the cellular distribution of the sugars. Here, we show that apple plantlets accumulate soluble sugars in leaf tissues under drought stress. Transcriptional profiling of stressed and control plantlets revealed differential expression of several plasma membrane- or vacuolar membrane-localized sugar transporter genes. Among these, four previously identified vacuolar sugar transporter (VST) genes (MdERDL6–1, MdERDL6–2, MdTST1 and MdTST2) showed higher expression under drought, suggesting their roles in response to drought stress. Promoter cis-elements analyses, yeast one-hybrid and dual-luciferase tests confirmed that the drought-induced transcription factor MdDREB2A could promote the expression of MdERDL6–1/−2 and MdTST1/2 by binding to their promoter regions. Moreover, overexpressing of each of these four MdVSTs alone in transgenic apple or Arabidopsis plants accumulated more soluble sugars and abscisic acid, and enhanced drought resistance. Furthermore, apple plants overexpressing MdERDL6–1 also showed reduced water potential, facilitated stomatal closure and reactive oxygen species scavenging under drought condition compared to control plants. Overall, our results suggest a potential strategy to enhance drought resistance and sugar accumulation in fruits through manipulating the genes involved in vacuolar sugar transport.
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来源期刊
Horticulture Research
Horticulture Research Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
11.20
自引率
6.90%
发文量
367
审稿时长
20 weeks
期刊介绍: Horticulture Research, an open access journal affiliated with Nanjing Agricultural University, has achieved the prestigious ranking of number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. As a leading publication in the field, the journal is dedicated to disseminating original research articles, comprehensive reviews, insightful perspectives, thought-provoking comments, and valuable correspondence articles and letters to the editor. Its scope encompasses all vital aspects of horticultural plants and disciplines, such as biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.
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